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AB248783

Anti-FECH antibody [EPR8312] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FECH antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Heme synthase, Protoheme ferro-lyase, FECH

1 Images
Western blot - Anti-FECH antibody [EPR8312] - BSA and Azide free (AB248783)
  • WB

Unknown

Western blot - Anti-FECH antibody [EPR8312] - BSA and Azide free (AB248783)

This data was developed using ab137042, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FECH antibody [EPR8312] (<a href='/en-us/products/primary-antibodies/fech-antibody-epr8312-ab137042'>ab137042</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate

Lane 2:

K562 cell lysate

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8312

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab248783 is the carrier-free version of ab137042.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FECH also known as ferrochelatase is a mitochondrial enzyme responsible for catalyzing the final step of heme biosynthesis. It inserts ferrous iron into protoporphyrin IX to form heme an essential component of hemoglobin myoglobin and various cytochromes. FECH has a molecular weight of approximately 43 kDa and is expressed in tissues with high heme demand like bone marrow and liver. Alternative names for FECH include protoporphyrinogen IX ferrochelatase and protoporphyrin ferro-lyase.
Biological function summary

FECH plays an important role in the production of heme facilitating the incorporation of iron into protoporphyrin IX. This reaction is not operating alone; FECH functions as part of multienzyme complexes in the mitochondria tightly regulated to meet cellular heme requirements. The activity of FECH ensures the proper functioning of heme-dependent proteins impacting oxygen transport and cellular respiration.

Pathways

FECH significantly contributes to the heme biosynthesis pathway. It interacts closely with other enzymes in the pathway such as aminolevulinate synthase (ALAS) and porphobilinogen deaminase essential for the step-wise production of heme. FECH's interaction with these proteins ensures the efficient flow of intermediates throughout the heme biosynthesis pathway ultimately impacting the synthesis of important heme proteins like cytochromes.

FECH mutations lead to erythropoietic protoporphyria (EPP) a condition characterized by sensitivity to sunlight and liver complications. The defective FECH reduces heme production efficiency causing protoporphyrin accumulation in red blood cells. FECH also interacts with proteins involved in mitochondrial function affecting iron metabolism and potentially linking to disorders such as sideroblastic anemia where abnormal erythropoiesis occurs due to defects in iron incorporation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the ferrous insertion into protoporphyrin IX and participates in the terminal step in the heme biosynthetic pathway.
See full target information FECH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 7:95 PubMed37723227

2023

Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia M Setayesh,Libere J Ndacayisaba,Kate E Rappard,Valerie Hennes,Luz Yurany Moreno Rueda,Guilin Tang,Pei Lin,Robert Z Orlowski,David E Symer,Elisabet E Manasanch,Stephanie N Shishido,Peter Kuhn
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com