JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248783

Anti-FECH antibody [EPR8312] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal FECH antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Heme synthase, Protoheme ferro-lyase, FECH

1 Images
Western blot - Anti-FECH antibody [EPR8312] - BSA and Azide free (AB248783)
  • WB

Unknown

Western blot - Anti-FECH antibody [EPR8312] - BSA and Azide free (AB248783)

This data was developed using ab137042, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FECH antibody [EPR8312] (<a href='/en-us/products/primary-antibodies/fech-antibody-epr8312-ab137042'>ab137042</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate

Lane 2:

K562 cell lysate

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8312

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Product details

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FECH also known as ferrochelatase is a mitochondrial enzyme responsible for catalyzing the final step of heme biosynthesis. It inserts ferrous iron into protoporphyrin IX to form heme an essential component of hemoglobin myoglobin and various cytochromes. FECH has a molecular weight of approximately 43 kDa and is expressed in tissues with high heme demand like bone marrow and liver. Alternative names for FECH include protoporphyrinogen IX ferrochelatase and protoporphyrin ferro-lyase.
Biological function summary

FECH plays an important role in the production of heme facilitating the incorporation of iron into protoporphyrin IX. This reaction is not operating alone; FECH functions as part of multienzyme complexes in the mitochondria tightly regulated to meet cellular heme requirements. The activity of FECH ensures the proper functioning of heme-dependent proteins impacting oxygen transport and cellular respiration.

Pathways

FECH significantly contributes to the heme biosynthesis pathway. It interacts closely with other enzymes in the pathway such as aminolevulinate synthase (ALAS) and porphobilinogen deaminase essential for the step-wise production of heme. FECH's interaction with these proteins ensures the efficient flow of intermediates throughout the heme biosynthesis pathway ultimately impacting the synthesis of important heme proteins like cytochromes.

FECH mutations lead to erythropoietic protoporphyria (EPP) a condition characterized by sensitivity to sunlight and liver complications. The defective FECH reduces heme production efficiency causing protoporphyrin accumulation in red blood cells. FECH also interacts with proteins involved in mitochondrial function affecting iron metabolism and potentially linking to disorders such as sideroblastic anemia where abnormal erythropoiesis occurs due to defects in iron incorporation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the ferrous insertion into protoporphyrin IX.
See full target information FECH
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com