Anti-FEN1 antibody [4D9]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FEN1 antibody [4D9] (AB314001)

Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with ab314001 at 1 : 25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [4D9] (AB314001)

Immunohistochemical analysis of ab314001 diluted at 1 : 77.5 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• WB

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Western blot - Anti-FEN1 antibody [4D9] (AB314001)

All lanes:

Western blot - Anti-FEN1 antibody [4D9] (ab314001) at 0.775 µg/mL

Lane 1:

HeLa whole cell lysate

Lane 2:

Raji whole cell lysate

Lane 3:

HepG2 whole cell lysate

Lane 4:

Jurkat whole cell lysate

Lane 5:

MCF7 whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 43 kDa

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