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AB153825

Anti-FEN1 antibody

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(4 Publications)

Rabbit Polyclonal FEN1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FEN1 aa 150 to C-terminus.

View Alternative Names

RAD2, FEN1, Flap endonuclease 1, FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-FEN1 antibody (AB153825)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FEN1 antibody (AB153825)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling FEN1 protein at nucleus using ab153825 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody used at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-FEN1 antibody (AB153825)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FEN1 antibody (AB153825)

Immunofluorescence analysis of paraformaldehyde fixed A549 cells labeling FEN1 with ab153825 at 1/200. The lower panel is merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody (AB153825)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody (AB153825)

Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue stained for FEN1 protein at nucleus with ab153825 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation - Anti-FEN1 antibody (AB153825)
  • IP

Supplier Data

Immunoprecipitation - Anti-FEN1 antibody (AB153825)

Samples were separated by 10 % SDS PAGE. The immunoprecipitated FEN1 protein was detected by ab153825 diluted at 1/500.

All lanes:

Immunoprecipitation - Anti-FEN1 antibody (ab153825) at 4 µg

Lane 1:

Jurkat whole cell extract at 30 µg

Lane 2:

Jurkat whole cell extract with Control IgG

Lane 3:

Jurkat whole cell extract with ab153825

Secondary

All lanes:

EasyBlot anti Rabbit IgG

Predicted band size: 43 kDa

false

Western blot - Anti-FEN1 antibody (AB153825)
  • WB

Supplier Data

Western blot - Anti-FEN1 antibody (AB153825)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus Enhanced.

All lanes:

Western blot - Anti-FEN1 antibody (ab153825) at 1/1000 dilution

All lanes:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 43 kDa

true

Western blot - Anti-FEN1 antibody (AB153825)
  • WB

Unknown

Western blot - Anti-FEN1 antibody (AB153825)

10% SDS-PAGE

All lanes:

Western blot - Anti-FEN1 antibody (ab153825) at 1/1000 dilution

All lanes:

PC12 whole cell lysate at 30 µg

Predicted band size: 43 kDa,50 kDa

false

Western blot - Anti-FEN1 antibody (AB153825)
  • WB

Unknown

Western blot - Anti-FEN1 antibody (AB153825)

10% SDS-PAGE

All lanes:

Western blot - Anti-FEN1 antibody (ab153825) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Lane 3:

BCL1 whole cell lysate at 30 µg

Lane 4:

Raw264.7 whole cell lysate at 30 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FEN1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P39748

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flap Endonuclease 1 known as FEN1 or FEN-1 is an important protein involved in DNA replication and repair. This protein shows a molecular weight of approximately 42 kDa. FEN1 functions as an endonuclease where it removes 5’ overhanging flaps in DNA products that occur during DNA replication and repair processes. It expresses in a wide range of human tissues with significant presence in the nucleus where DNA synthesis mainly occurs.
Biological function summary

FEN1 plays an important role in the Okazaki fragment maturation during lagging strand synthesis and long-patch base excision repair. FEN1 is not a solitary actor; it forms a part of larger complexes essential for maintaining genomic stability. It works closely with proliferating cell nuclear antigen (PCNA) to enhance its function and to interact with other proteins essential for the stability of the DNA.

Pathways

DNA replication and repair processes heavily rely on the activity of FEN1. It’s a component of the DNA replication fork protection and repair pathway and interacts with other proteins such as DNA polymerase and DNA ligase I to complete the repair process. Efficient DNA replication ensures accurate genomic duplication preventing mutations that could lead to severe cellular malfunction.

FEN1 is linked to the development of cancer and neurodegenerative diseases like Huntington's disease. FEN1 mutations or malfunction can result in genomic instability and an increased risk of tumorigenesis. In the context of neurodegenerative diseases deficiencies in FEN1 activity may disrupt the repair of oxidative DNA damage which is an important feature of disorders like Huntington's. These insights highlight the need for further research into FEN1's role and interactions to develop potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
See full target information FEN1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature 640:1093-1102 PubMed40205037

2025

Comprehensive interrogation of synthetic lethality in the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

John Fielden,Sebastian M Siegner,Danielle N Gallagher,Markus S Schröder,Maria Rosaria Dello Stritto,Simon Lam,Lena Kobel,Moritz F Schlapansky,Stephen P Jackson,Petr Cejka,Marco Jost,Jacob E Corn

Contrast media & molecular imaging 2022:6177809 PubMed35711530

2022

Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7.

Applications

Unspecified application

Species

Unspecified reactive species

Jinmei Xu,Qingsong Zhao,Xu Han,Zheqi Zhang,Jiahui Qu,Zhifeng Cheng

Journal of ovarian research 15:32 PubMed35246224

2022

miR-4324 inhibits ovarian cancer progression by targeting FEN1.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Wu,Youliang Yan,Jialin Yuan,Mengze Luo,Yingjian Wang

Nucleic acids research 49:4864-4876 PubMed33928345

2021

Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Asada,Fumika Sakaue,Tetsuya Nagata,Ji-Chun Zhang,Kie Yoshida-Tanaka,Aya Abe,Makiko Nawa,Kazutaka Nishina,Takanori Yokota
View all publications
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