AB133311

Anti-FEN1 antibody [EPR4459(2)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal FEN1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

RAD2, FEN1, Flap endonuclease 1, FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)] (AB133311)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)] (AB133311)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labelled with ab133311 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)] (AB133311)

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labelled with ab133311 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-FEN1 antibody [EPR4459(2)] (AB133311)

All lanes:

Western blot - Anti-FEN1 antibody [EPR4459(2)] (ab133311) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4459(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flap Endonuclease 1 known as FEN1 or FEN-1 is an important protein involved in DNA replication and repair. This protein shows a molecular weight of approximately 42 kDa. FEN1 functions as an endonuclease where it removes 5’ overhanging flaps in DNA products that occur during DNA replication and repair processes. It expresses in a wide range of human tissues with significant presence in the nucleus where DNA synthesis mainly occurs.

Biological function summary

FEN1 plays an important role in the Okazaki fragment maturation during lagging strand synthesis and long-patch base excision repair. FEN1 is not a solitary actor; it forms a part of larger complexes essential for maintaining genomic stability. It works closely with proliferating cell nuclear antigen (PCNA) to enhance its function and to interact with other proteins essential for the stability of the DNA.

Pathways

DNA replication and repair processes heavily rely on the activity of FEN1. It’s a component of the DNA replication fork protection and repair pathway and interacts with other proteins such as DNA polymerase and DNA ligase I to complete the repair process. Efficient DNA replication ensures accurate genomic duplication preventing mutations that could lead to severe cellular malfunction.

FEN1 is linked to the development of cancer and neurodegenerative diseases like Huntington's disease. FEN1 mutations or malfunction can result in genomic instability and an increased risk of tumorigenesis. In the context of neurodegenerative diseases deficiencies in FEN1 activity may disrupt the repair of oxidative DNA damage which is an important feature of disorders like Huntington's. These insights highlight the need for further research into FEN1's role and interactions to develop potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

See full target information FEN1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of general medicine 14:6477-6485 PubMed34675615

2021

The Clinical Significance of the Expression of FEN1 in Primary Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxian Zhong,Yunqing Wang,Hongxiang Wei,Meifang Chen,Huangfeng Lin,Zhen Huang,Jinlong Huang,Shenglin Wang,Jianhua Lin

Molecular medicine reports 13:386-92 PubMed26718738

2016

Flap endonuclease 1 silencing is associated with increasing the cisplatin sensitivity of SGC‑7901 gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhong Xie,Kejia Wang,Daorong Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FEN1 antibody [EPR4459(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)] (AB133311)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)] (AB133311)

Western blot - Anti-FEN1 antibody [EPR4459(2)] (AB133311)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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