Rabbit Polyclonal WDFY1 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human WDFY1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49.97% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WDFY1. Database link Q8IWB7
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/5000 | Notes - |
Target data
Function
Positively regulates TLR3- and TLR4-mediated signaling pathways by bridging the interaction between TLR3 or TLR4 and TICAM1. Promotes TLR3/4 ligand-induced activation of transcription factors IRF3 and NF-kappa-B, as well as the production of IFN-beta and inflammatory cytokines (PubMed:25736436).
Alternative names
FENS1, KIAA1435, WDF1, ZFYVE17, WDFY1, WD repeat and FYVE domain-containing protein 1, FYVE domain-containing protein localized to endosomes 1, Phosphoinositide-binding protein 1, WD40- and FYVE domain-containing protein 1, Zinc finger FYVE domain-containing protein 17, FENS-1
Rabbit Polyclonal WDFY1 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human WDFY1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49.97% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WDFY1. Database link Q8IWB7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FENS1 also known as WDFY1 is a protein that plays a role in several cellular processes. It has a mass of approximately 80 kDa. The protein is predominantly expressed in several tissues including the brain heart and skeletal muscle. FENS1 is known for its involvement in cellular signaling and serves as a scaffolding protein interacting with other molecules to facilitate various cellular functions.
- Biological function summary
FENS1/WDFY1 impacts immune system responses. It is part of a complex that includes other proteins suggesting its role in cellular defense mechanisms against pathogens. The protein aids in orchestrating the interactions between signaling molecules and receptors promoting effective communications within immune cells. Its involvement is critical for maintaining a balanced immune response and it affects both innate and adaptive immunity functions.
- Pathways
FENS1/WDFY1 interacts with a few signaling pathways particularly the Toll-like receptor signaling. This pathway is essential for the recognition and response to microbial components. FENS1/WDFY1 collaborates with related proteins such as MyD88 to ensure proper signal transduction which is necessary for activating immune responses. Another pathway related to FENS1/WDFY1 includes NF-kB signaling which is important for immune regulation and inflammation processes.
- Associated diseases and disorders
FENS1/WDFY1 has associations with immune-related conditions such as autoimmune disorders and inflammatory diseases. The dysfunction or altered expression of FENS1/WDFY1 can disrupt normal immune signaling leading to conditions like rheumatoid arthritis. Moreover relations between FENS1/WDFY1 and other proteins like TLR4 emphasize its potential role in modulating inflammatory responses seen in these diseases. Understanding these connections can provide insights into therapeutic targets for better managing these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-FENS1/WDFY1 Antibody (ab316954)
All lanes: Western blot - Anti-FENS1/WDFY1 Antibody (ab316954) at 1/2000 dilution
Lane 1: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2: THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse kidney tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Observed band size: 47 kDa
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