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AB191060

Anti-FER antibody

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(4 Publications)

Rabbit Polyclonal FER antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human FER aa 500-550.

View Alternative Names

TYK3, FER, Tyrosine-protein kinase Fer, Feline encephalitis virus-related kinase FER, Fujinami poultry sarcoma/Feline sarcoma-related protein Fer, Proto-oncogene c-Fer, Tyrosine kinase 3, p94-Fer

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FER antibody (AB191060)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FER antibody (AB191060)

Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue labeling FER with ab191060 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FER antibody (AB191060)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FER antibody (AB191060)

Immunohistochemical analysis of paraffin-embedded rat intestine tissue labeling FER with ab191060 at 1 μg/ml.

Western blot - Anti-FER antibody (AB191060)
  • WB

Supplier Data

Western blot - Anti-FER antibody (AB191060)

All lanes:

Western blot - Anti-FER antibody (ab191060) at 0.5 µg/mL

Lane 1:

Rat testis tissue lysate

Lane 2:

Rat ovary tissue lysate

Predicted band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FER aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

P16591

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p>The detection limit for FER is approximately 5ng/lane under reducing conditions.</p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FER protein also known as mouse FER or FER-1 functions as a non-receptor tyrosine kinase within the Fes/Fps subfamily. It has a molecular mass of approximately 94 kDa. FER is expressed across various tissues notably in epithelial cells and is also present in hematopoietic and neural cells. Its kinase activity regulates cell signaling affecting processes like cell proliferation differentiation and survival.
Biological function summary

FER acts as a regulator in signaling pathways essential for cellular communication and structure maintenance. FER interacts within cellular complexes modulating the cytoskeleton and intercellular junctions. It influences actin remodeling impacting cell adhesion and motility. FER's involvement in such activities makes it important for maintaining epithelial integrity and cellular response to external stimuli.

Pathways

FER plays a role in the PI3K/Akt pathway and the MAPK/ERK pathway. These pathways are important for cell growth survival and proliferation. FER interacts with other proteins like STAT3 and JAK2 integrating signals that drive these pathways. By modulating key signaling events FER influences cellular functions and responses to environmental changes.

FER associates with cancer and cardiovascular diseases. Aberrant FER expression and activity have been found in various tumors linking it to cancer progression and metastasis. In cardiovascular disease FER's role in regulating endothelial function connects it to vascular abnormalities. Its interaction with proteins like VEGFR further implicates FER in the pathogenesis of these conditions serving as a potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus.
See full target information FER
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:667800 PubMed33987186

2021

Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η.

Applications

Unspecified application

Species

Unspecified reactive species

Jiarui Liu,Yang Han,Shunfeng Hu,Yiqing Cai,Juan Yang,Shuai Ren,Yi Zhao,Tiange Lu,Xiangxiang Zhou,Xin Wang

Molecular medicine reports 19:5219-5226 PubMed31059042

2019

Role of feline sarcoma‑related protein in the viability and apoptosis of bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Hu,Zhiwen Guo,Jifei Xu,Xiangbao Mei,Manhua Bi,Fang Jiang,Dexin Yu,Caiyun Zhong

Gene therapy 25:359-375 PubMed29907877

2018

Electroporation-mediated delivery of FER gene enhances innate immune response and improves survival in a murine model of pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Vladislav Dolgachev,Sreehari Panicker,Sanjay Balijepalli,Lane Kelly McCandless,Yue Yin,Samantha Swamy,M V Suresh,Matthew J Delano,Mark R Hemmila,Krishnan Raghavendran,David Machado-Aranda

Gene therapy 23:785-796 PubMed27454317

2016

Electroporation-mediated delivery of the FER gene in the resolution of trauma-related fatal pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

V A Dolgachev,R Goldberg,M V Suresh,B Thomas,N Talarico,M R Hemmila,K Raghavendran,D Machado-Aranda
View all publications
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