Anti-Ferritin antibody [RM1036]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin antibody [RM1036] (AB287968)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling FTH1 with ab287968 at 1/100 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the human liver. The section was incubated with ab287968 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Ferritin antibody [RM1036] (AB287968)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling FTH1 with ab287968 at 1/50 dilution (1ug)/ red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Ferritin antibody [RM1036] (AB287968)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling FTH1 with ab287969 at 1/51 dilution (1ug)/ red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2001 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin antibody [RM1036] (AB287968)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling FTH1 with ab287968 at 1/5000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the mouse liver. The section was incubated with ab287968 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin antibody [RM1036] (AB287968)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling FTH1 with ab287968 at 1/5000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the rat liver. The section was incubated with ab287968 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• WB

Supplier Data

Western blot - Anti-Ferritin antibody [RM1036] (AB287968)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 1-5 : 3min

RIPA is the lysis buffer routinely used for WB (lanes 2 – 6). Preparation of lysates using the 1% SDS hot lysis method is recommended if bands are difficult to detect in RIPA buffer lysate.

All lanes:

Western blot - Anti-Ferritin antibody [RM1036] (ab287968) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate prepared from 1% SDS Hot lysis method at 20 µg

Lane 2:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate prepared from RIPA lysis method at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 5:

J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-Ferritin antibody [RM1036] (AB287968)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 1-3 : 50s
Lane 4-8 : 3min

All lanes:

Western blot - Anti-Ferritin antibody [RM1036] (ab287968) at 1/1000 dilution

Lane 1:

Human Liver at 20 µg

Lane 2:

Mouse liver at 20 µg

Lane 3:

Rat Liver at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false