Anti-Ferritin antibody [RM1036]

7 product images

• 

  Western blot - Anti-Ferritin antibody [RM1036] (ab287968)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  Exposure time: Lane 1-3: 50s
  Lane 4-8: 3min

  All lanes: Western blot - Anti-Ferritin antibody [RM1036] (ab287968) at 1/1000 dilution

  Lane 1: Human Liver at 20 µg

  Lane 2: Mouse liver at 20 µg

  Lane 3: Rat Liver at 20 µg

  Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 5: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

  Lane 6: Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate at 20 µg

  Lane 7: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

  Lane 8: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 21 kDa

  Observed band size: 21 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin antibody [RM1036] (ab287968)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling FTH1 with ab287968 at 1/100 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the human liver. The section was incubated with ab287968 at 4°C overnight. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Flow Cytometry (Intracellular) - Anti-Ferritin antibody [RM1036] (ab287968)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling FTH1 with ab287968 at 1/50 dilution (1ug)/ red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

• 

  Western blot - Anti-Ferritin antibody [RM1036] (ab287968)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  Exposure time: Lane 1-5: 3min

  RIPA is the lysis buffer routinely used for WB (lanes 2 – 6). Preparation of lysates using the 1% SDS hot lysis method is recommended if bands are difficult to detect in RIPA buffer lysate.

  All lanes: Western blot - Anti-Ferritin antibody [RM1036] (ab287968) at 1/1000 dilution

  Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate prepared from 1% SDS Hot lysis method at 20 µg

  Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate prepared from RIPA lysis method at 20 µg

  Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 4: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

  Lane 5: J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 21 kDa

  Observed band size: 21 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin antibody [RM1036] (ab287968)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling FTH1 with ab287968 at 1/5000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the mouse liver. The section was incubated with ab287968 at 4°C overnight. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Flow Cytometry (Intracellular) - Anti-Ferritin antibody [RM1036] (ab287968)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling FTH1 with Anti-Ferritin antibody [RM1036] - BSA and Azide free ab287969 at 1/51 dilution (1ug)/ red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2001 dilution was used as the secondary antibody.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin antibody [RM1036] (ab287968)

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling FTH1 with ab287968 at 1/5000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the rat liver. The section was incubated with ab287968 at 4°C overnight. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)