AB194947

Anti-Ferritin Heavy Chain antibody - C-terminal

Name: Anti-Ferritin Heavy Chain antibody - C-terminal (ab194947) | Abcam
Brand: Abcam Limited
SKU: AB194947
Price: 645 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Ferritin Heavy Chain antibody. C-terminal. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FTH1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FTH, FTHL6, OK/SW-cl.84, PIG15, FTH1, Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody - C-terminal (AB194947)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody - C-terminal (AB194947)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling Ferritin Heavy Chain using ab194947 at a 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Ferritin Heavy Chain antibody - C-terminal (AB194947)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Ferritin Heavy Chain antibody - C-terminal (AB194947)

Intracellular Flow Cytometry analysis of HepG2 cells labeling Ferritin Heavy Chain (bottom histogram), using ab194947 at a 1/10 dilution, and negative control cells (top histogram). FITC-conjugated Goat anti-Rabbit secondary antibodies were used for the analysis.

Supplier Data

Western blot - Anti-Ferritin Heavy Chain antibody - C-terminal (AB194947)

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody - C-terminal (ab194947) at 1/1000 dilution

All lanes:

A2058 cell lysate at 35 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human FTH1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P02794

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody." }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed : 9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed : 9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed : 24695223, PubMed : 26436293).

See full target information FTH1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Poultry science 103:103912 PubMed38943808

2024

Follicular fluid-derived exosomal HMOX1 promotes granulosa cell ferroptosis involved in follicular atresia in geese (Anser cygnoides).

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Youluan Jiang,Xiaoqian Dong,Shuwen Luo,Guoyu Jiao,Kaiqi Weng,Qiang Bao,Yang Zhang,Wanwipa Vongsangnak,Guohong Chen,Qi Xu

BMC infectious diseases 21:228 PubMed33639860

2021

Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kehkshan Jabeen,Uzma Malik,Sajid Mansoor,Shaheen Shahzad,Saadia Zahid,Aneela Javed

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ferritin Heavy Chain antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody - C-terminal (AB194947)

Flow Cytometry (Intracellular) - Anti-Ferritin Heavy Chain antibody - C-terminal (AB194947)

Western blot - Anti-Ferritin Heavy Chain antibody - C-terminal (AB194947)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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