Anti-Ferritin Heavy Chain antibody [EPR18878]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR18878] (AB183781)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Ferritin Heavy Chain with ab183781 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatic cell of the rat liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR18878] (AB183781)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Ferritin Heavy Chain with ab183781 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on the mouse kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR18878] (AB183781)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Ferritin Heavy Chain with ab183781 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatic cell of the mouse liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Ferritin Heavy Chain antibody [EPR18878] (AB183781)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time :

Lane 1 : 30 seconds
Lanes 2-9 : 3 minutes.

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR18878] (ab183781) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Lane 6:

Rat spleen lysate at 10 µg

Lane 7:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 8:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 9:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-Ferritin Heavy Chain antibody [EPR18878] (AB183781)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR18878] (ab183781) at 1/1000 dilution

Lane 1:

Mouse liver lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 12 kDa,21 kDa,39 kDa,49 kDa,54 kDa,69 kDa,84 kDa,93 kDa

Observed band size: 12 kDa,21 kDa,40 kDa,45 kDa,54 kDa,70 kDa,72 kDa,86 kDa,93 kDa

false

Exposure time: 30s

• WB

CiteAb

Western blot - Anti-Ferritin Heavy Chain antibody [EPR18878] (AB183781)

Ferritin Heavy Chain western blot using anti-Ferritin Heavy Chain antibody [EPR18878] ab183781. Publication image and figure legend from Lissner, M. M., Cumnock, K., et al., 2020, Elife, PubMed 33021470.

ab183781 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab183781 please see the product overview.

Heme metabolism appears largely normal inAhr-/-mice during malaria.(A) Msp1 expression in perfused kidneys from Ahr+/- and Ahr-/- mice on 8 DPI (normalized to Hmbs using the ddCT method, n = 6 per group). (B) Representative images of Perls Prussian blue-stained kidney cortex from Pc-infected Ahr+/- and Ahr-/- mice on 8 DPI. The blue signal (arrows) indicates accumulation of iron. (C) Western blot showing levels of heme metabolism genes in liver and kidney tissue from Pc-infected Ahr+/- and Ahr-/- mice on 8 DPI. (D) qRT-PCR analysis of heme metabolism genes in liver tissue from Pc-infected Ahr+/- and Ahr-/- mice on 8 DPI (n = 3–5 per condition). p-Values in A and D were determined using a Wilcox test. *p<0.05. These experiments were performed once.Figure 4—figure supplement 2—source data 1.Source data for Figure 4—figure supplement 2.Source data for Figure 4—figure supplement 2.

false