Skip to main content

Anti-Ferritin heavy chain antibody [EPR3004Y] ab75973 is a rabbit monoclonal antibody that is used in Ferritin heavy chain western blotting, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR3004Y is the most widely used clone for Ferritin heavy chain on the market and is cited in >160 publications
- Specificity confirmed with FTH1 knockout cell line validation


Images

Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973), expandable thumbnail
  • Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973), expandable thumbnail
  • Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973), expandable thumbnail
  • Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Tested
Not recommended
Expected
Expected
Rat
Tested
Not recommended
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/2000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/2000

Notes

-

Species

Human

Dilution info

1/1000 - 1/2000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

We recommend following our protocol when testing ICC and recommend using lower dilution in samples having low expression level of Ferritin.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

1 product for Alternative Version

Target data

Function

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Alternative names

Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein, PIG15, OK/SW-cl.84, FTHL6, FTH, FTH1

Recommended products

Anti-Ferritin heavy chain antibody [EPR3004Y] ab75973 is a rabbit monoclonal antibody that is used in Ferritin heavy chain western blotting, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR3004Y is the most widely used clone for Ferritin heavy chain on the market and is cited in >160 publications
- Specificity confirmed with FTH1 knockout cell line validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3004Y

Purification technique

Affinity purification Protein A

Specificity

We recommend following our protocol when testing ICC and recommend using lower dilution in samples having low expression level of Ferritin.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973), expandable thumbnail

    Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973)

    Lanes 1-3: Merged signal (red and green). Green - ab75973 observed at 21 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab75973 Anti-Ferritin heavy chain antibody [EPR3004Y] was shown to specifically react with Ferritin heavy chain in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human FTH1 knockout HEK-293T cell line ab266581 (knockout cell lysate Human FTH1 knockout HEK-293T cell lysate ab256924) was used. Wild-type and Ferritin knockout samples were subjected to SDS-PAGE. ab75973 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: FTH1 knockout HEK293T cell lysate at 20 µg

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

    This data was developed using the same antibody clone in a different buffer formulation (ab75973).

    Lanes 1-3: Merged signal (red and green). Green - ab75973 observed at 21 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab75973 Anti-Ferritin heavy chain antibody [EPR3004Y] was shown to specifically react with Ferritin heavy chain in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human FTH1 knockout HEK-293T cell line ab266581 (knockout cell lysate Human FTH1 knockout HEK-293T cell lysate ab256924) was used. Wild-type and Ferritin knockout samples were subjected to SDS-PAGE. ab75973 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973), expandable thumbnail

    Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973)

    Observed MW: 21 kDA

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973) at 1/2000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

    Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 21 kDa

  • Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973), expandable thumbnail

    Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973) at 1/10000 dilution

    All lanes: Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973), expandable thumbnail

    Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973)

    All lanes: Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973) at 1/2000 dilution

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

    Lane 2: SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

    Lane 3: Human brain lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 21 kDa

    Observed band size: 19 kDa, 21 kDa

  • Flow Cytometry (Intracellular) - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Ferritin heavy chain with ab75973 at 1/50 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973)

    Immunofluorescence staining of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized Jurkat cells (human T cell leukemia T lymphocyte from peripheral blood) with ab75973 at a working dilution of 1/100. The secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, was used at a dilution of 1/1000. The counterstain, Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used at a dilution of 1/200.

    Confocal image showing positive staining in Jurkat cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973), expandable thumbnail

    Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973)

    False colour image of Western blot: Anti-Ferritin heavy chain antibody [EPR3004Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab75973 was shown to bind specifically to Ferritin heavy chain. A band was observed at 24 kDa in wild-type MCF7 cell lysates with no signal observed at this size in FTH1 knockout cell line ab269493 (knockout cell lysate ab269655). To generate this image, wild-type and FTH1 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (ab75973) at 1/1000 dilution

    Lane 1: Wild-type MCF7 cell lysate at 20 µg

    Lane 2: FTH1 knockout MCF7 cell lysate at 20 µg

    Lane 3: Wild-type HEK-293T ab255553 at 20 µg

    Lane 4: FTH1 knockout HEK-293T ab260185 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 21 kDa

    Observed band size: 24 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com