Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) is a rabbit monoclonal antibody detecting Ferritin Heavy Chain in Western Blot, IHC-P. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
- Trusted since 2009
Images
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img16952.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42952.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972)")
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img123938.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972)")
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img143293.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972)")
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img29874.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (AB75972)")
Publications
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- Current issues in molecular biology 45:9868-98862023Hydroxycitric Acid Alleviated Lung Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress and Ferroptosis through the Hif-1α Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Long Lu,Cong-Kuan Song,Shi-Shi Zou,Shi-Ze Pan,Kai Lai,Ning Li,Qing GengPubMed 38132462
- Bone research 11:582023Combining single-cell RNA sequencing and population-based studies reveals hand osteoarthritis-associated chondrocyte subpopulations and pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Li,Xiaofeng Jiang,Yongbing Xiao,Yuqing Zhang,Weiya Zhang,Michael Doherty,Jacquelyn Nestor,Changjun Li,Jing Ye,Tingting Sha,Houchen Lyu,Jie Wei,Chao Zeng,Guanghua LeiPubMed 37914703
- Frontiers in endocrinology 14:11775472023Ferroptosis and its potential role in gestational diabetes mellitus: updated evidence from pathogenesis to therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhao,Qianqian Gao,Baoxuan Li,Yang Wang,Yue WangPubMed 37664858
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229852023A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanhuan Lv,Yijia Wang,Chenxiao Zhen,Junyu Liu,Xin Chen,Gejing Zhang,Wei Yao,Huijie Guo,Yunpeng Wei,Shenghang Wang,Jiancheng Yang,Peng ShangPubMed 37249350
- Cell death discovery 9:1382023Defective NCOA4-dependent ferroptosis in senescent fibroblasts retards diabetic wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuerong Wei,Mengqian Liu,Zijun Zheng,Shengxiang Yu,Lei Huang,Jun Ma,Yanbin Gao,Yujie Peng,Lianglong Chen,Rongwei Tan,Zhending She,Lei YangPubMed 37117222
- EBioMedicine 90:1045092023Sensitive magnetic particle imaging of haemoglobin degradation for the detection and monitoring of intraplaque haemorrhage in atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Tong,Yingqian Zhang,Hui Hui,Xin Feng,Bin Ning,Tengfei Yu,Wei Wang,Yaxin Shang,Guanghao Zhang,Suhui Zhang,Feng Tian,Wen He,Yundai Chen,Jie TianPubMed 36905783
- Cancers 15:2023Ferroptosis Inducers Kill Mesenchymal Stem Cells Affected by Neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangze Li,Qi Wang,Chencheng Xu,Lei Zhang,Jiquan Zhou,Jingchun Lv,Min Xu,Dapeng JiangPubMed 36831642
- International journal of molecular sciences 24:2023Regulatory Mechanism between Ferritin and Mitochondrial Reactive Oxygen Species in Spinal Ligament-Derived Cells from Ossification of Posterior Longitudinal Ligament Patient.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJong Tae Kim,Yonggoo Kim,Ji Yeon Kim,Seungok Lee,Myungshin Kim,Dong Wook JekarlPubMed 36769191
- Annals of translational medicine 10:13902023Ferroptosis-related genes with post-transcriptional regulation mechanisms in hepatocellular carcinoma determined by bioinformatics and experimental validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenfei Zhu,Cheng Gao,Qiuqi Feng,Haitao Guan,Jianjun Wu,Hrishikesh Samant,Fan Yang,Xuehao WangPubMed 36660631
- Oncology reports 48:20225‑Aminolevurinic acid inhibits the proliferation of bladder cancer cells by activating heme synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasushi Nakai,Yoshihiro Tatsumi,Shunta Hori,Yosuke Morizawa,Kota Iida,Kenta Onishi,Makito Miyake,Yuki Oda,Takuya Owari,Tomomi Fujii,Sayuri Onishi,Nobumichi Tanaka,Kiyohide FujimotoPubMed 36082808
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed:24695223, PubMed:26436293).
Alternative names
FTH, FTHL6, OK/SW-cl.84, PIG15, FTH1, Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein
Recommended products
Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) is a rabbit monoclonal antibody detecting Ferritin Heavy Chain in Western Blot, IHC-P. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
- Trusted since 2009
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3005Y
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognizes only the Ferritin heavy chain.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of Ferritin Heavy Chain?
Anti-Ferritin Heavy Chain [EPR3005Y] (ab75972) specifically detects a band for Ferritin Heavy Chain (UniProt: P02794) at a molecular weight of 21kDa.
Trusted by the scientific community
Anti-Ferritin Heavy Chain [EPR3005Y] (ab75972) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR3005Y] also available for your convenience:
ab75972, Carrier free - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free ab247418, APC - APC Anti-Ferritin Heavy Chain antibody [EPR3005Y] ab319237, PE - PE Anti-Ferritin Heavy Chain antibody [EPR3005Y] ab319385, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Ferritin Heavy Chain antibody [EPR3005Y] ab319519, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Ferritin Heavy Chain antibody [EPR3005Y] ab319632, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Ferritin Heavy Chain antibody [EPR3005Y] ab319736, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Ferritin Heavy Chain antibody [EPR3005Y] ab319878, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Ferritin Heavy Chain antibody [EPR3005Y] ab321705
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img16952.jpg "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
Blocking and diluting buffer: 5% NFDM/TBST
ab75972 works better in some lysates prepared from 1% SDS Hot lysis method. For Lysate preparation protocol, please refer to the protocol section of the website and/or here.
All lanes: Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/1000 dilution
Lane 1: MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from 1% SDS Hot lysis method. at 20 µg
Lane 2: MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from RIPA lysis method at 20 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from 1% SDS Hot lysis method at 20 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from RIPA lysis method at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42952.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
ab75972 staining Ferritin Heavy Chain in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img123938.jpg "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
Lanes 1 - 3: Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution
Lane 4: Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution
Lane 5: Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution
Lane 1: Human liver lysate at 10 µg
Lane 2: SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg
Lanes 3 - 4: Human brain lysate at 10 µg
Lane 5: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img143293.jpg "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
All lanes: Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution
All lanes: Human fetal liver at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 21 kDa
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img29874.jpg "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
All lanes: Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img444647.jpg "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) + si -scramble whole cell lysate, 20 μg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) + si - Ferritin Heavy Chain whole cell lysate, 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 20s
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img445976.jpg "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
Ferritin Heavy Chain Western blot staining using rabbit Anti-Ferritin Heavy Chain antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/1000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: Ferritin Heavy Chain KO 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 60s
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img444647.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-epr3005y-ab75972--western-blot-img445976.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)")
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