Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

Unpurified ab75972 showing positive staining in normal breast tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

Unpurified ab75972 at 1/100 dilution staining Ferritin Heavy Chain in Human kidney tissue, using a HRP/AP polymerized secondary antibody.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

ab75972 staining Ferritin Heavy Chain in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

Unpurified ab75972 showing positive staining in normal liver tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

Unpurified ab75972 showing positive staining in normal tonsil tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (<a href='/en-us/products/primary-antibodies/ferritin-heavy-chain-antibody-epr3005y-ab75972'>ab75972</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Ferritin Heavy Chain KO 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Exposure time: 60s

• WB

Lab

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (<a href='/en-us/products/primary-antibodies/ferritin-heavy-chain-antibody-epr3005y-ab75972'>ab75972</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) + si -scramble whole cell lysate, 20 μg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) + si - Ferritin Heavy Chain whole cell lysate, 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Exposure time: 20s

• WB

Lab

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

ab75972 works better in some lysates prepared from 1% SDS Hot lysis method.

For Lysate preparation protocol, please refer to the protocol section of the website and/or here.

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (<a href='/en-us/products/primary-antibodies/ferritin-heavy-chain-antibody-epr3005y-ab75972'>ab75972</a>) at 1/1000 dilution

Lane 1:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from 1% SDS Hot lysis method. at 20 µg

Lane 2:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from RIPA lysis method at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from 1% SDS Hot lysis method at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from RIPA lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (<a href='/en-us/products/primary-antibodies/ferritin-heavy-chain-antibody-epr3005y-ab75972'>ab75972</a>) at 1/5000 dilution

All lanes:

Human fetal liver at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 21 kDa

false

• WB

Lab

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (<a href='/en-us/products/primary-antibodies/ferritin-heavy-chain-antibody-epr3005y-ab75972'>ab75972</a>) at 1/2000 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

false

• WB

Unknown

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (AB247418)

This data was developed using ab75972, the same antibody clone in a different buffer formulation.

Lanes 1 - 3:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418) at 1/500 dilution

Lane 4:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418) at 1/2000 dilution

Lane 5:

Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418) at 1/5000 dilution

Lane 1:

Human liver lysate at 10 µg

Lane 2:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

Lanes 3 - 4:

Human brain lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false