Rabbit Multiclonal Ferritin Heavy Chain antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FTH1.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody [RP23040130] (AB308115), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-rp23040130-ab308115--immunocytochemistry-immunofluorescence-img406930.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody [RP23040130] (AB308115)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- Synthetic Peptide within Human FTH1. Database link P02794
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed:24695223, PubMed:26436293).
Alternative names
FTH, FTHL6, OK/SW-cl.84, PIG15, FTH1, Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein
Recommended products
Rabbit Multiclonal Ferritin Heavy Chain antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FTH1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- Synthetic Peptide within Human FTH1. Database link P02794
- Clone number
- RP23040130
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody [RP23040130] (ab308115), expandable thumbnail](https://content.abcam.com/products/images/ferritin-heavy-chain-antibody-rp23040130-ab308115--immunocytochemistry-immunofluorescence-img406930.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody [RP23040130] (ab308115)")
Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody [RP23040130] (ab308115)
For immunofluorescence analysis, HepG2 cells were fixed and permeabilized for detection of Ferritin Heavy Chain using ab308115 at 2 µg/mL and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000).
Panel a) shows representative cells that were stained for detection and localization of Ferritin Heavy Chain protein (green)
Panel b) is stained for nuclei (blue) using DAPI
Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution
Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Ferritin Heavy Chain
Panel e) represents control cells with no primary antibody to assess background
The images were captured at 60X magnification
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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