Anti-Ferritin Light Chain antibody (ab69090) is a rabbit polyclonal antibody that is used to detect Ferritin Light Chain in Western Blot. Suitable for Human, Mouse, Rat samples.
- Over 90 publications
- Trusted since 2009
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- Scientific reports 13:202542023Hypobaric hypoxia induces iron mobilization from liver and spleen and increases serum iron via activation of ghrelin/GHSR1a/MAPK signalling pathway in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanping Yang,Jiayan Li,Jianan Hu,Xiaoyu Yuan,Jie Ding,Hui Jiang,Guohua Wang,Qianqian LuoPubMed 37985861
- Experimental & molecular medicine 55:2433-24442023Single-cell characterization of macrophages in uveal melanoma uncovers transcriptionally heterogeneous subsets conferring poor prognosis and aggressive behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Li,Lanfang Sun,Yanan Wang,Yixin Cen,Jingting Zhao,Qianling Liao,Wencan Wu,Jie Sun,Meng ZhouPubMed 37907747
- Molecular neurobiology 61:2313-23352023Sevoflurane Exposure Induces Neuronal Cell Ferroptosis Initiated by Increase of Intracellular Hydrogen Peroxide in the Developing Brain via ER Stress ATF3 Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiheng Kang,Meihua Piao,Nan Liu,Wanping Gu,Chunsheng FengPubMed 37874483
- Microbiome 11:2122023The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZirun Jin,Yuzhuo Yang,Yalei Cao,Qi Wen,Yu Xi,Jianxing Cheng,Qiancheng Zhao,Jiaming Weng,Kai Hong,Hui Jiang,Jing Hang,Zhe ZhangPubMed 37752615
- Methods in molecular biology (Clifton, N.J.) 2712:211-2222023Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Zhong,Ruochan ChenPubMed 37578709
- Food & function 14:3673-36852023Intestinal iron bio-accessibility changes by Lignin and the subsequent impact on cell metabolism and intestinal microbiome communities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard D Horniblow,Prachi Pathak,Maryam Eshrati,Gladys O Latunde-Dada,Chris TselepisPubMed 36970974
- Wellcome open research 7:2672023Defective iron homeostasis and hematological abnormalities in Niemann-Pick disease type C1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOscar C W Chen,Stephan Siebel,Alexandria Colaco,Elena-Raluca Nicoli,Nick Platt,Dawn Shepherd,Stephanie Newman,Andrew E Armitage,Nicole Y Farhat,George Seligmann,Claire Smith,David A Smith,Alaa Abdul-Sada,Mylvaganam Jeyakumar,Hal Drakesmith,Forbes D Porter,Frances M PlattPubMed 37065726
- Cell death & disease 14:2112023Fatostatin induces ferroptosis through inhibition of the AKT/mTORC1/GPX4 signaling pathway in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayang Cai,Zhang Ye,Yuanyuan Hu,Liguo Ye,Lun Gao,Yixuan Wang,Qian Sun,Shiao Tong,Shenqi Zhang,Liquan Wu,Ji'an Yang,Qianxue ChenPubMed 36966152
- Investigative ophthalmology & visual science 64:222023Ferritin But Not Iron Increases in Retina Upon Systemic Iron Overload in Diabetic and Iron-Dextran Injected Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAina Bonet,Judit Pampalona,Eduard Jose-Cunilleras,Víctor Nacher,Jesús RubertePubMed 36912597
- eLife 12:2023Impaired iron recycling from erythrocytes is an early hallmark of aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatryk Slusarczyk,Pratik Kumar Mandal,Gabriela Zurawska,Marta Niklewicz,Komal Chouhan,Raghunandan Mahadeva,Aneta Jończy,Matylda Macias,Aleksandra Szybinska,Magdalena Cybulska-Lubak,Olga Krawczyk,Sylwia Herman,Michal Mikula,Remigiusz Serwa,Małgorzata Lenartowicz,Wojciech Pokrzywa,Katarzyna Mleczko-SaneckaPubMed 36719185
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-FoFr | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Not recommended |
| Mouse | Expected | Expected | Not recommended |
| Rat | Tested | Expected | Not recommended |
| Dog | Predicted | Predicted | Not recommended |
| Orangutan | Predicted | Predicted | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.5-1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes - |
Species Mouse | Dilution info 0.5-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Orangutan | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Orangutan | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Dog, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).
Alternative names
Ferritin light chain, Ferritin L subunit, FTL
Recommended products
Anti-Ferritin Light Chain antibody (ab69090) is a rabbit polyclonal antibody that is used to detect Ferritin Light Chain in Western Blot. Suitable for Human, Mouse, Rat samples.
- Over 90 publications
- Trusted since 2009
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Ferritin Light Chain antibody (ab69090) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of Ferritin Light Chain?
Anti-Ferritin Light Chain (ab69090) specifically detects a band for Ferritin Light Chain (UniProt: P02792) at a molecular weight of 19kDa.
Trusted by the scientific community
Anti-Ferritin Light Chain (ab69090) was first used in a scientific publication in 2009 and has been cited over 90 times in peer-reviewed journals.
Reviewed by scientists
Anti-Ferritin Light Chain (ab69090) has over 30 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-Ferritin Light Chain antibody (ab69090)
All lanes: Western blot - Anti-Ferritin Light Chain antibody (ab69090) at 1 µg/mL
Lane 1: Human liver tissue lysate - total protein (ab29889) at 10 µg
Lane 2: Liver (Rat) Tissue Lysate at 10 µg
Lane 3: Western blot - Mouse spleen tissue lysate - total protein (Mouse spleen tissue lysate - total protein ab29293) at 10 µg
Lane 4: Testis (Rat) Tissue Lysate - normal tissue (ab29388) at 10 µg
Lane 5: Liver (Mouse) Tissue Lysate at 10 µg
Lane 6: Ovary (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
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Product protocols
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- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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