Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Suitable for ELISA, WB, Flow Cyt (Intra) and reacts with Synthetic peptide, Human samples. Cited in 1 publication.
Images
![Western blot - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (AB243096), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-ab243096--western-blot-img1920.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (AB243096)")
![Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (AB243096), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-ab243096--flow-cytometry-intracellular-img78873.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (AB243096)")
![ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (AB243096), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-ab243096--elisa-img98885.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (AB243096)")
Publications
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- American journal of physiology. Renal physiology 317:F1563-F15712019Parenterial iron sucrose-induced renal preconditioning: differential ferritin heavy and light chain expression in plasma, urine, and internal organs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAli C Johnson,Ted Gooley,Alvaro Guillem,Jeff Keyser,Henrik Rasmussen,Bhupinder Singh,Richard A ZagerPubMed 31608670
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ELISA | WB | IHC-P | ICC/IF | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Synthetic peptide | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 0.1-1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
Alternative names
Ferritin light chain, Ferritin L subunit, FTL
Recommended products
Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Suitable for ELISA, WB, Flow Cyt (Intra) and reacts with Synthetic peptide, Human samples. Cited in 1 publication.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
CPT-R10-58-11
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-ab243096--western-blot-img1920.jpg "Western blot - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)")
Western blot - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)
Exposure time : Lane 1-4: 26 seconds, Lane 5: 5.5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096) at 1/1000 dilution
Lane 1: NCI-H460 (human lung cancer cell line) whole cell lysate at 20 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 5: Human brain lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 19 kDa
![Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-ab243096--flow-cytometry-intracellular-img78873.jpg "Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)")
Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling Ferritin Light Chain with ab243096 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-ab243096--elisa-img98885.jpg "ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)")
ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)
50ng CPTAC-14d (1000ng/mL, 50μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000ng/mL) of ab243096 (50μL) were incubated with CPTAC-14d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50μL) into each well and incubating for another 40 minutes. After washing, 50μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
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