Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Carrier free. Suitable for ELISA, WB, Flow Cyt (Intra) and reacts with Synthetic peptide, Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (AB245128), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-bsa-and-azide-free-ab245128--flow-cytometry-intracellular-img78873.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (AB245128)")
![ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (AB245128), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-bsa-and-azide-free-ab245128--elisa-img98885.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (AB245128)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ELISA | WB | IHC-P | ICC/IF | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Not recommended | Not recommended | Not recommended | Tested |
| Synthetic peptide | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).
Alternative names
Ferritin light chain, Ferritin L subunit, FTL
Recommended products
Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Carrier free. Suitable for ELISA, WB, Flow Cyt (Intra) and reacts with Synthetic peptide, Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
CPT-R10-58-11
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab245128 is the carrier-free version of ab241329.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-bsa-and-azide-free-ab245128--flow-cytometry-intracellular-img78873.jpg "Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)")
Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling Ferritin Light Chain with Anti-Ferritin Light Chain antibody [CPT-R10-58-11] ab243096 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ferritin Light Chain antibody [CPT-R10-58-11] ab243096).
![ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-cpt-r10-58-11-bsa-and-azide-free-ab245128--elisa-img98885.jpg "ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)")
ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)
50ng CPTAC-14d (1000ng/mL, 50μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000ng/mL) of Anti-Ferritin Light Chain antibody [CPT-R10-58-11] ab243096 (50μL) were incubated with CPTAC-14d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50μL) into each well and incubating for another 40 minutes. After washing, 50μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ferritin Light Chain antibody [CPT-R10-58-11] ab243096).
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