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Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (AB322536), expandable thumbnail
  • Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (AB322536), expandable thumbnail
  • Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (AB322536), expandable thumbnail
  • Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (AB322536), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).

Alternative names

Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25846-85

Purification technique

Affinity purification Protein A

Specificity

This antibody has 0.09% cross reaction with human Ferritin Heavy Chain.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab322536 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Ferritin Light Chain. The recommended pair for sandwich ELISA is:

Capture: Anti-Ferritin Light Chain antibody [EPR25846-17] - BSA and Azide free (Capture) ab322535, Human Ferritin Light Chain Capture Antibody (unconjugated)

Detector: ab322536, Human Ferritin Light Chain Detector Antibody (unconjugated)

The reference range value is 0.16-10 ng/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (ab322536), expandable thumbnail

    Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (ab322536)

    Interpolated concentration of native Ferritin Light Chain was measured at different sample concentrations. Undiluted samples are as follows: Hela cell extract (10 ug/mL) and HepG2 cell extract (100 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (ab322536), expandable thumbnail

    Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (ab322536)

    Interpolated concentration of native Ferritin Light Chain was measured at different sample concentrations. Undiluted sample is as follows: human plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (ab322536), expandable thumbnail

    Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (ab322536)

    Sandwich ELISA of Human Ferritin Light Chain Antibody Pair - BSA and Azide free ab322559 with the capture antibody Anti-Ferritin Light Chain antibody [EPR25846-17] - BSA and Azide free (Capture) ab322535 dilution at 2 ug/mL and detector antibody ab322536 dilution at 0.5 ug/mL.

  • Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (ab322536), expandable thumbnail

    Sandwich ELISA - Anti-Ferritin Light Chain antibody [EPR25846-85] - BSA and Azide free (Detector) (ab322536)

    Interpolated concentration of native Ferritin Light Chain was measured at different sample concentrations. Undiluted samples are as follows: human urine 50% and human milk 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com