Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 22 publications.
Images
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-ab109373--western-blot-img1450.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373)")
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-ab109373--western-blot-img54801.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373)")
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-ab109373--western-blot-img111136.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373)")
Publications
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- Theranostics 13:3794-38132023Ferritin light chain promotes the reprogramming of glioma immune microenvironment and facilitates glioma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongjiang Li,Chao Yang,Yanfei Wei,Xueyuan Li,Wei Jiang,Yiran Xu,Lifeng Li,Rongqun Guo,Di Chen,Peng Gao,Haohao Zhang,Hui Qin,Zhenyu Zhang,Xianzhi Liu,Dongming YanPubMed 37441589
- Frontiers in cellular neuroscience 17:11360702023Effects of liraglutide on astrocyte polarization and neuroinflammation in db/db mice: focus on iron overload and oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Ren An,Jun-Tong Liu,Xiao-Meng Gao,Qing-Feng Wang,Gui-Yan Sun,Jia-Nan Su,Chi Zhang,Jia-Xiang Yu,Yu-Feng Yang,Yan ShiPubMed 37323581
- Cell death & disease 14:1612023Cdh5-mediated Fpn1 deletion exerts neuroprotective effects during the acute phase and inhibitory effects during the recovery phase of ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiwen Zheng,Xin Guo,Shaomeng Kang,Zhongda Li,Tian Tian,Jianhua Li,Fudi Wang,Peng Yu,Shiyang Chang,Yan-Zhong ChangPubMed 36841833
- International journal of nanomedicine 18:843-8592023Huperzine A-Liposomes Efficiently Improve Neural Injury in the Hippocampus of Mice with Chronic Intermittent Hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Yue Yang,Lina Geng,Ronghui Li,Ji-Xian Song,Cui-Ling Jia,Ji-Ren An,Meng-Fan Sun,Shan Xu,Ya-Jing Guo,Yashuo Zhao,En-Sheng JiPubMed 36824413
- Antioxidants (Basel, Switzerland) 12:2023CHIR99021 Maintenance of the Cell Stemness by Regulating Cellular Iron Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingying Han,Yong He,Xiaofang Jin,Jiayi Xie,Peng Yu,Guofen Gao,Shiyang Chang,Jianhua Zhang,Yan-Zhong ChangPubMed 36829936
- Cell death & disease 13:10062022The divergent effects of astrocyte ceruloplasmin on learning and memory function in young and old mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhong-Da Li,Haiyan Li,Shaomeng Kang,Yan-Ge Cui,Huiwen Zheng,Peina Wang,Kang Han,Peng Yu,Yan-Zhong ChangPubMed 36443285
- Pharmaceutical biology 60:1449-14572022A novobiocin derivative, XN4, triggers ferroptosis in gastric cancer cells via the activation of NOX4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongrong Li,Bin Yin,Deyu Zeng,Zhenyang LiuPubMed 35938505
- Life (Basel, Switzerland) 12:2022Caffeine Decreases Hepcidin Expression to Alleviate Aberrant Iron Metabolism under Inflammation by Regulating the IL-6/STAT3 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhong-Da Li,Meng-Yu Geng,Song-Rui Dou,Xuan Wang,Zi-Han Zhang,Yan-Zhong ChangPubMed 35888113
- Antioxidants (Basel, Switzerland) 11:2022Overexpression of Mitochondrial Ferritin Enhances Blood-Brain Barrier Integrity Following Ischemic Stroke in Mice by Maintaining Iron Homeostasis in Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeina Wang,Qianqian Ren,Mengtong Shi,Yuanyuan Liu,Huiyuan Bai,Yan-Zhong ChangPubMed 35883748
- Cancer cell international 21:7092021FTH promotes the proliferation and renders the HCC cells specifically resist to ferroptosis by maintaining iron homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanye Hu,Chaoting Zhou,Qiangan Jing,Yancun Li,Jing Yang,Chen Yang,Luyang Wang,Jiayu Hu,Huanjuan Li,Hairui Wang,Chen Yuan,Yi Zhou,Xueying Ren,Xiangmin Tong,Jing Du,Ying WangPubMed 34965856
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Predicted | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
12 products for Alternative Product
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).
Alternative names
Ferritin light chain, Ferritin L subunit, FTL
Recommended products
Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 22 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5260
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognizes the light chain of Ferritin only.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-ab109373--western-blot-img1450.jpg "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)")
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
Lanes 1-2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FTL knockout HeLa cell line ab265533 (knockout cell lysate Human FTL knockout HeLa cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/2000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Ferritin knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FTL knockout HeLa cell line (Human FTL knockout HeLa cell line ab265533)
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-ab109373--western-blot-img54801.jpg "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)")
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
Lanes 1 - 2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. ab109373 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 20 kDa, 49 kDa
Observed band size: 50 kDa
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-ab109373--western-blot-img111136.jpg "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)")
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
All lanes: Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution
Lane 1: Fetal liver lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Predicted band size: 20 kDa
Observed band size: 19 kDa
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