Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (AB239971), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-bsa-and-azide-free-ab239971--western-blot-img1450.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (AB239971)")
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (AB239971), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-bsa-and-azide-free-ab239971--western-blot-img54801.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (AB239971)")
![Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (AB239971), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-bsa-and-azide-free-ab239971--flow-cytometry-intracellular-img122651.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (AB239971)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Expected | Not recommended | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Predicted |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
Alternative names
Ferritin light chain, Ferritin L subunit, FTL
Recommended products
Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR5260
- Purification technique
Affinity purification Protein A
- Specificity
This antibody recognizes the light chain of Ferritin only.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab239971 is the carrier-free version of Anti-Ferritin Light Chain antibody [EPR5260] ab109373.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-bsa-and-azide-free-ab239971--western-blot-img1450.jpg "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)")
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Ferritin Light Chain antibody [EPR5260] ab109373).
Lanes 1-2: Merged signal (red and green). Green - Anti-Ferritin Light Chain antibody [EPR5260] ab109373 observed at 20 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-Ferritin Light Chain antibody [EPR5260] ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FTL knockout HeLa cell line ab265533 (knockout cell lysate Human FTL knockout HeLa cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. Anti-Ferritin Light Chain antibody [EPR5260] ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (Anti-Ferritin Light Chain antibody [EPR5260] ab109373) at 1/2000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Ferritin knockout HeLa cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Lanes 1-2: Merged signal (red and green). Green - Anti-Ferritin Light Chain antibody [EPR5260] ab109373 observed at 20 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-Ferritin Light Chain antibody [EPR5260] ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FTL knockout HeLa cell line ab265533 (knockout cell lysate Human FTL knockout HeLa cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. Anti-Ferritin Light Chain antibody [EPR5260] ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-bsa-and-azide-free-ab239971--western-blot-img54801.jpg "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)")
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
Lanes 1 - 2: Merged signal (red and green). Green - Anti-Ferritin Light Chain antibody [EPR5260] ab109373 observed at 20 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Ferritin Light Chain antibody [EPR5260] ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Anti-Ferritin Light Chain antibody [EPR5260] ab109373 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ferritin Light Chain antibody [EPR5260] ab109373).
All lanes: Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (Anti-Ferritin Light Chain antibody [EPR5260] ab109373) at 1/10000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 20 kDa, 49 kDa
Observed band size: 50 kDa
![Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-epr5260-bsa-and-azide-free-ab239971--flow-cytometry-intracellular-img122651.jpg "Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)")
Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
Intracellular flow cytometric analysis of permeabilized HeLa cells using Anti-Ferritin Light Chain antibody [EPR5260] ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ferritin Light Chain antibody [EPR5260] ab109373).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com