Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free

• WB

Lab

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (AB239971)

Lanes 1 - 2 : Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. ab109373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109373).

All lanes:

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (<a href='/en-us/products/primary-antibodies/ferritin-light-chain-antibody-epr5260-ab109373'>ab109373</a>) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 20 kDa,49 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (AB239971)

This data was developed using the same antibody clone in a different buffer formulation (ab109373).

Lanes 1-2 : Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265533 (knockout cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (<a href='/en-us/products/primary-antibodies/ferritin-light-chain-antibody-epr5260-ab109373'>ab109373</a>) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Ferritin knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FTL knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ftl-knockout-hela-cell-line-ab265533'>ab265533</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false