Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

Mouse Monoclonal Ferritin Light Chain antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FTL aa 1 to C-terminus.

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Images

Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (AB218400), expandable thumbnail

Publications

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human FTL aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P02792

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	Protein Array
Human	Tested	Tested	Expected
Recombinant full length protein - Human	Not recommended	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 0.25-0.5 µg/mL	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1-2 µg/mL	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information FTL

Function

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).

Alternative names

Ferritin light chain, Ferritin L subunit, FTL

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human FTL aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P02792
Clone number: FTL/1386
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: ab218400 is purified from Bioreactor Concentrate by Protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Product promise

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9 product images

Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
Lanes 1- 2: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) observed at 50 kDa.
ab218400 was shown to react with Ferritin Light Chain in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human FTL knockout HeLa cell line ab265534 (knockout cell lysate Human FTL knockout HeLa cell lysate ab256927) was used. Wild-type HeLa and Ferritin Light Chain knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab218400 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^®680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FTL knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FTL knockout HeLa cell line (Human FTL knockout HeLa cell line ab265534)
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular carcinoma tissue labelling Ferritin Light Chain with ab218400 at 0.5 μg/mL (30 minutes at room temperature). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 2 µg/mL
All lanes: Human kidney tissue lysate
Predicted band size: 20 kDa
Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
ab218400 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
ab218400 Anti-Ferritin Light Chain antibody [FTL/1386] was shown to specifically react with Ferritin Light Chain in wild-type HeLa cells (ab255928). Loss of signal was observed when knockout cell line Human FTL knockout HeLa cell line ab265534 (knockout cell lysate Human FTL knockout HeLa cell lysate ab256927) was used. Wild-type and Ferritin Light Chain knockout samples were subjected to SDS-PAGE. ab218400 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FTL knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FTL knockout HeLa cell line (Human FTL knockout HeLa cell line ab265533)
Secondary
All lanes: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
Lanes 1 - 4: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
ab218400 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. ab218400 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate at 20 µg
Lane 3: A549 whole cell lysate at 20 µg
Lane 4: Hep G2 whole cell lysate at 20 µg
Predicted band size: 20 kDa
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 0.2 µg/mL
Lanes 1 - 2: HeLa cell lysate
Lane 3: Human liver lysate
Lane 4: Human testis lysate
Predicted band size: 20 kDa, 31 kDa
Observed band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218400 at 0.2 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218400 at 0.2 μg/ml.