Mouse Monoclonal Ferritin Light Chain antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FTL aa 1 to C-terminus.
Images
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--western-blot-img148853.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136930.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866)")
![Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--protein-array-img1236.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866)")
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--western-blot-img2814.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866)")
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--western-blot-img4868.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (AB218866)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human FTL aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P02792
Reactivity data
Select an application| IHC-P | WB | Protein Array | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.20000 µg/mL | Notes (Primary incubation for 30 min at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.20000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
5 products for Alternative Product
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).
Alternative names
Ferritin light chain, Ferritin L subunit, FTL
Recommended products
Mouse Monoclonal Ferritin Light Chain antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FTL aa 1 to C-terminus.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human FTL aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P02792
- Carrier free
- Yes
- Clone number
- FTL/1386
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
ab218866 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--western-blot-img148853.jpg "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Ferritin Light Chain antibody [FTL/1386] ab218400).
Lanes 1- 2: Merged signal (red and green). Green - Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 observed at 20 kDa. Red - Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) observed at 50 kDa.
Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 was shown to react with Ferritin Light Chain in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human FTL knockout HeLa cell line ab265534 (knockout cell lysate Human FTL knockout HeLa cell lysate ab256927) was used. Wild-type HeLa and FTL knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (Anti-Ferritin Light Chain antibody [FTL/1386] ab218400) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FTL knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FTL knockout HeLa cell line (Human FTL knockout HeLa cell line ab265534)
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136930.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218866 at 0.2 μg/ml.
![Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--protein-array-img1236.jpg "Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)
This data was produced with Anti-Ferritin Light Chain antibody [FTL/1386] ab218400, the same antibody in a different formulation with BSA and Azide.
Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--western-blot-img2814.jpg "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Ferritin Light Chain antibody [FTL/1386] ab218400).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 observed at 20 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate at 20 µg
Lane 3: A549 whole cell lysate at 20 µg
Lane 4: Hep G2 whole cell lysate at 20 µg
Predicted band size: 20 kDa
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--western-blot-img4868.jpg "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)
This data was developed using the same antibody clone in a different buffer formation (Anti-Ferritin Light Chain antibody [FTL/1386] ab218400)
Lanes 1-2: Merged signal (red and green). Green - Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 observed at 20 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.
Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FTL knockout HeLa cell line ab265533 (knockout lysate Human FTL knockout HeLa cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. Anti-Ferritin Light Chain antibody [FTL/1386] ab218400 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4° at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772)and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (Anti-Ferritin Light Chain antibody [FTL/1386] ab218400) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FTL knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FTL knockout HeLa cell line (Human FTL knockout HeLa cell line ab265533)
SecondaryAll lanes: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133278.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218866 at 0.2 μg/ml.
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--western-blot-img74952.jpg "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)
All lanes: Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866) at 0.2 µg/mL
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Human liver lysate
Lane 4: Human testis lysate
Predicted band size: 20 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133278.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866), expandable thumbnail](https://content.abcam.com/products/images/ferritin-light-chain-antibody-ftl-1386-bsa-and-azide-free-ab218866--western-blot-img74952.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)")
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