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AB108418

Anti-FES antibody [EPR3230(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal FES antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FPS, FES, Tyrosine-protein kinase Fes/Fps, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, Proto-oncogene c-Fes, Proto-oncogene c-Fps, p93c-fes

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FES antibody [EPR3230(2)] (AB108418)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FES antibody [EPR3230(2)] (AB108418)

ab108418, at a 1/100 dilution, staining FES in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-FES antibody [EPR3230(2)] (AB108418)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-FES antibody [EPR3230(2)] (AB108418)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FES with purified ab108418 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-FES antibody [EPR3230(2)] (AB108418)
  • WB

Unknown

Western blot - Anti-FES antibody [EPR3230(2)] (AB108418)

All lanes:

Western blot - Anti-FES antibody [EPR3230(2)] (ab108418) at 1/1000 dilution

Lane 1:

TF-1 lysate at 10 µg

Lane 2:

U937 lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Predicted band size: 93 kDa

false

  • Carrier free

    Anti-FES antibody [EPR3230(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3230(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FES protein also known as Feline Sarcoma Oncogene is a cytoplasmic tyrosine kinase with a molecular mass of approximately 93 kDa. It plays a role in regulating processes like cell growth differentiation and survival. FES is mainly expressed in hematopoietic cells endothelial cells and some neural tissues. Its structure includes SH2 and SH3 domains allowing the protein to interact with other cellular components.
Biological function summary

FES protein is involved in signal transduction pathways that control various physiological functions. It does not form part of any giant complexes rather it serves as a relay point for signals in cellular contexts. The kinase activity of FES is important for ensuring cellular processes such as actin remodeling an important process for cell movement and shape changes.

Pathways

The FES protein participates in the RAS and MAPK signaling pathways. These pathways are important for transmitting extracellular signals that govern cell fate decisions. FES interacts with proteins such as GRB2 and SOS1 in these pathways to modulate downstream effects and enable effective response to cellular stimuli.

Aberrant FES signaling has links to cancer and inflammatory disorders. Dysregulation of FES can contribute to several types of cancer including leukemia due to its effect on cell proliferation and survival. In inflammation FES interaction with proteins like STAT3 may promote pathological inflammatory responses therefore implicating FES in diseases such as rheumatoid arthritis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein kinase that acts downstream of cell surface receptors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, cell attachment and cell spreading. Plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Acts down-stream of the activated FCER1 receptor and the mast/stem cell growth factor receptor KIT. Plays a role in the regulation of mast cell degranulation. Plays a role in the regulation of cell differentiation and promotes neurite outgrowth in response to NGF signaling. Plays a role in cell scattering and cell migration in response to HGF-induced activation of EZR. Phosphorylates BCR and down-regulates BCR kinase activity. Phosphorylates HCLS1/HS1, PECAM1, STAT3 and TRIM28.
See full target information FES
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40063400

2025

The asymmetric expression of HSPA2 in blastomeres governs the first embryonic cell-fate decision.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayin Gao,Jiawei Wang,Shiyu Liu,Jinzhu Song,Chuanxin Zhang,Boyang Liu,Keliang Wu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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