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Rabbit Recombinant Monoclonal Hemoglobin subunit gamma-2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] (AB283313), expandable thumbnail
  • Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] (AB283313), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-fetal hemoglobin antibody [EPR23381-254] (AB283313), expandable thumbnail
  • Immunoprecipitation - Anti-fetal hemoglobin antibody [EPR23381-254] (AB283313), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-fetal hemoglobin antibody [EPR23381-254] (AB283313), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.

Alternative names

Hemoglobin subunit gamma-2, Gamma-2-globin, Hb F Ggamma, Hemoglobin gamma-2 chain, Hemoglobin gamma-G chain, HBG2

Recommended products

Rabbit Recombinant Monoclonal Hemoglobin subunit gamma-2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23381-254
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Fetal hemoglobin also known as fetal Hb or HbF is a form of hemoglobin found in fetuses and neonates. HbF is composed of two alpha and two gamma globin chains and its molecular weight is around 64000 Daltons. This hemoglobin variant is mainly expressed in the red blood cells of the fetal liver and bone marrow. Expression of fetal hemoglobin typically declines after birth when beta globin chains replace gamma chains to form adult hemoglobin (HbA). However low levels of fetal hemoglobin can persist into adulthood in some individuals.

Biological function summary

Fetal hemoglobin has a higher affinity for oxygen compared to adult hemoglobin. This characteristic allows efficient transfer of oxygen from maternal to fetal circulation which is vital for fetal development. Fetal hemoglobin is not part of any complex but its unique structure is adapted to its function in the fetal environment. The alpha and gamma globin chains together modulate its oxygen-binding properties ensuring proper oxygenation during fetal life.

Pathways

The production and transition of fetal hemoglobin are linked to the globin gene switching pathway. This process involves the silencing of gamma globin genes and activation of beta globin genes postnatally a transition integral to producing adult hemoglobin. BCL11A and KLF1 are transcription factors that play essential roles in regulating this gene switching. Both proteins influence the repression of gamma globin genes therefore promoting the expression of beta globin.

Associated diseases and disorders

Fetal hemoglobin is significant in conditions like sickle cell disease and beta thalassemia. In these disorders elevated levels of fetal hemoglobin can ameliorate symptoms by compensating for defective adult hemoglobin. Patients with higher fetal hemoglobin levels typically exhibit milder forms of these diseases. Therapeutic approaches aim to reactivate fetal hemoglobin expression through the modulation of proteins like BCL11A KLF1 and others involved in hemoglobin switching.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313), expandable thumbnail

    Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 seconds

    These rec proteins were made in house.
    Samples loaded are extracts of E.coli expressing
    HBG2, HBG1, HBE1, HBB or HBD.

    All lanes: Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313) at 1/1000 dilution

    Lane 1: His-tagged human HBG2 recombinant protein (aa2-147) at 0.01 µg

    Lane 2: His-tagged human HBG1 recombinant protein (aa2-147) at 0.01 µg

    Lane 3: His-tagged human HBE1 recombinant protein (aa3-147) at 0.01 µg

    Lane 4: His-tagged human HBB recombinant protein (aa3-147) at 0.01 µg

    Lane 5: His-tagged human HBD recombinant protein (aa3-147) at 0.01 µg

    Secondary

    Lanes 1 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Lanes 1 - 5: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 16 kDa

    Observed band size: 15 kDa

  • Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313), expandable thumbnail

    Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: HeLa (PMID: 17910885).

    Extra bands around 40 kDa and 150 kDa can be observed in some adult tissue lysates.

    Exposure time: 59 seconds

    All lanes: Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HEL (human erythroleukemia erythroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 16 kDa

    Observed band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized K-562 cells labelling fetal hemoglobin with ab283313 at 1/50 (10.92 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in K-562 cells and no staining in HeLa cells.

    Negative control: HeLa (PMID: 17910885) is observed.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunoprecipitation - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313), expandable thumbnail

    Immunoprecipitation - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313)

    fetal hemoglobin was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with ab283313 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283313 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug

    Lane 2: ab283313 IP in K-562 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab283313 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    All lanes: Immunoprecipitation - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313)

    Predicted band size: 16 kDa

    Observed band size: 14 kDa

  • Flow Cytometry (Intracellular) - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-fetal hemoglobin antibody [EPR23381-254] (ab283313)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / K-562 (Human chronic myelogenous leukemia lymphoblast, Right) cells labelling fetal hemoglobin with ab283313 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HeLa (PMID: 17910885).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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