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Rabbit Recombinant Monoclonal Hemoglobin subunit gamma-2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] - BSA and Azide free (AB283333), expandable thumbnail
  • Western blot - Anti-fetal hemoglobin antibody [EPR23381-254] - BSA and Azide free (AB283333), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-fetal hemoglobin antibody [EPR23381-254] - BSA and Azide free (AB283333), expandable thumbnail
  • Immunoprecipitation - Anti-fetal hemoglobin antibody [EPR23381-254] - BSA and Azide free (AB283333), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-fetal hemoglobin antibody [EPR23381-254] - BSA and Azide free (AB283333), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.

    Alternative names

    Hemoglobin subunit gamma-2, Gamma-2-globin, Hb F Ggamma, Hemoglobin gamma-2 chain, Hemoglobin gamma-G chain, HBG2

    Recommended products

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    Rabbit Recombinant Monoclonal Hemoglobin subunit gamma-2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

    Alternative names

    Hemoglobin subunit gamma-2, Gamma-2-globin, Hb F Ggamma, Hemoglobin gamma-2 chain, Hemoglobin gamma-G chain, HBG2

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR23381-254

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    ab283333 is the carrier-free version of Anti-fetal hemoglobin antibody [EPR23381-254] ab283313.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    Fetal hemoglobin also known as fetal Hb or HbF is a form of hemoglobin found in fetuses and neonates. HbF is composed of two alpha and two gamma globin chains and its molecular weight is around 64000 Daltons. This hemoglobin variant is mainly expressed in the red blood cells of the fetal liver and bone marrow. Expression of fetal hemoglobin typically declines after birth when beta globin chains replace gamma chains to form adult hemoglobin (HbA). However low levels of fetal hemoglobin can persist into adulthood in some individuals.

    Biological function summary

    Fetal hemoglobin has a higher affinity for oxygen compared to adult hemoglobin. This characteristic allows efficient transfer of oxygen from maternal to fetal circulation which is vital for fetal development. Fetal hemoglobin is not part of any complex but its unique structure is adapted to its function in the fetal environment. The alpha and gamma globin chains together modulate its oxygen-binding properties ensuring proper oxygenation during fetal life.

    Pathways

    The production and transition of fetal hemoglobin are linked to the globin gene switching pathway. This process involves the silencing of gamma globin genes and activation of beta globin genes postnatally a transition integral to producing adult hemoglobin. BCL11A and KLF1 are transcription factors that play essential roles in regulating this gene switching. Both proteins influence the repression of gamma globin genes therefore promoting the expression of beta globin.

    Associated diseases and disorders

    Fetal hemoglobin is significant in conditions like sickle cell disease and beta thalassemia. In these disorders elevated levels of fetal hemoglobin can ameliorate symptoms by compensating for defective adult hemoglobin. Patients with higher fetal hemoglobin levels typically exhibit milder forms of these diseases. Therapeutic approaches aim to reactivate fetal hemoglobin expression through the modulation of proteins like BCL11A KLF1 and others involved in hemoglobin switching.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com