AB137096

Anti-fetal hemoglobin antibody [EPR9708(B)]

RabMAb
Recombinant
What is this?

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(10 Publications)

Rabbit Recombinant Monoclonal HBG1 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

PRO2979, HBG1, Hemoglobin subunit gamma-1, Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain

2 Images

Supplier Data

Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (AB137096)

Blocking and Diluting Buffer : 5% NFDM/TBST

Exposure time : 3 min

All lanes:

Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution

Lane 1:

Human red blood cell at 20 µg

Lane 2:

K562 (human lymphoblast from chronic myelogenous leukemia) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

Predicted band size: 16 kDa

false

Lab

Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (AB137096)

All lanes:

Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 16 kDa

Observed band size: 13 kDa

false

Carrier free

Anti-fetal hemoglobin antibody [EPR9708(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9708(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises Hemoglobin gamma-1/2 chain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fetal hemoglobin also known as fetal Hb or HbF is a form of hemoglobin found in fetuses and neonates. HbF is composed of two alpha and two gamma globin chains and its molecular weight is around 64000 Daltons. This hemoglobin variant is mainly expressed in the red blood cells of the fetal liver and bone marrow. Expression of fetal hemoglobin typically declines after birth when beta globin chains replace gamma chains to form adult hemoglobin (HbA). However low levels of fetal hemoglobin can persist into adulthood in some individuals.

Biological function summary

Fetal hemoglobin has a higher affinity for oxygen compared to adult hemoglobin. This characteristic allows efficient transfer of oxygen from maternal to fetal circulation which is vital for fetal development. Fetal hemoglobin is not part of any complex but its unique structure is adapted to its function in the fetal environment. The alpha and gamma globin chains together modulate its oxygen-binding properties ensuring proper oxygenation during fetal life.

Pathways

The production and transition of fetal hemoglobin are linked to the globin gene switching pathway. This process involves the silencing of gamma globin genes and activation of beta globin genes postnatally a transition integral to producing adult hemoglobin. BCL11A and KLF1 are transcription factors that play essential roles in regulating this gene switching. Both proteins influence the repression of gamma globin genes therefore promoting the expression of beta globin.

Fetal hemoglobin is significant in conditions like sickle cell disease and beta thalassemia. In these disorders elevated levels of fetal hemoglobin can ameliorate symptoms by compensating for defective adult hemoglobin. Patients with higher fetal hemoglobin levels typically exhibit milder forms of these diseases. Therapeutic approaches aim to reactivate fetal hemoglobin expression through the modulation of proteins like BCL11A KLF1 and others involved in hemoglobin switching.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.

See full target information HBG1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Experimental hematology & oncology 14:47 PubMed40156013

2025

A one-base therapeutic insertion in the HBG2 distal promoter reactivates γ-globin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuqin Bao,Yuanyi Gao,Xiaoyi Chen,Zhongju Wang,Xiaoqin Feng,Liren Wang,Jing Du,Yuhua Ye,Feijing Chen,Li Du,Aihua Yin,Xiangmin Xu

Molecular therapy. Nucleic acids 35:102371 PubMed39640014

2024

Efficient and correction of hemoglobin Constant Spring mutation by prime editing in human hematopoietic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Congwen Shao,Qing Liu,Jinchao Xu,Jianxiang Zhang,Chengpeng Zhang,Ye Xin,Yuhua Ye,Bin Lin,Xinhua Zhang,Li Cheng,Xiangmin Xu,Peng Xu

iScience 27:109172 PubMed38414864

2024

Disruption of mitochondrial energy metabolism is a putative pathogenesis of Diamond-Blackfan anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Rudan Xiao,Lijuan Zhang,Zijuan Xin,Junwei Zhu,Qian Zhang,Guangmin Zheng,Siyun Chu,Jing Wu,Lu Zhang,Yang Wan,Xiaojuan Chen,Weiping Yuan,Zhaojun Zhang,Xiaofan Zhu,Xiangdong Fang

Science advances 10:eadj9479 PubMed38295180

2024

Folate depletion induces erythroid differentiation through perturbation of de novo purine synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Adam G Maynard,Nancy K Pohl,Annabel P Mueller,Boryana Petrova,Alan Y L Wong,Peng Wang,Andrew J Culhane,Jeannette R Brook,Leah M Hirsch,Ngoc Hoang,Orville Kirkland,Tatum Braun,Sarah Ducamp,Mark D Fleming,Hojun Li,Naama Kanarek

Journal of immunology research 2022:8440422 PubMed35942209

2022

Transmembrane Protein ANTXR1 Regulates -Globin Expression by Targeting the Wnt/-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Jin,Zhaojun Zhang,Yuanyuan Han,Di Li,Juan Liu,Minmin Jiang,Junwei Zhu,Ryo Kurita,Yukio Nakamura,Fangfang Hu,Yongjie Xu,Xiangdong Fang,Shengwen Huang,Zhaolin Sun

Nucleic acids research 49:9711-9723 PubMed34379783

2021

Transcriptional silencing of fetal hemoglobin expression by NonO.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Li,Mengxia Chen,Biru Liu,Peifen Lu,Xiang Lv,Xiang Zhao,Shuaiying Cui,Peipei Xu,Yukio Nakamura,Ryo Kurita,Bing Chen,David C S Huang,De-Pei Liu,Ming Liu,Quan Zhao

Cell reports 32:107993 PubMed32755585

2020

ATF4 Regulates MYB to Increase γ-Globin in Response to Loss of β-Globin.

Applications

Unspecified application

Species

Unspecified reactive species

Mandy Y Boontanrart,Markus S Schröder,Gautier M Stehli,Marija Banović,Stacia K Wyman,Rachel J Lew,Matteo Bordi,Benjamin G Gowen,Mark A DeWitt,Jacob E Corn

BioMed research international 2017:9496058 PubMed29333458

2017

SOX6 Downregulation Induces -Globin in Human -Thalassemia Major Erythroid Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Yongrong Lai,Jun Luo,Lin Luo,Rongrong Liu,Zhenfang Liu,Weihua Zhao

Scientific reports 7:202 PubMed28303002

2017

Reciprocal regulation of γ-globin expression by exo-miRNAs: Relevance to γ-globin silencing in β-thalassemia major.

Applications

Species

Unspecified reactive species

Kuo-Ting Sun,Yu-Nan Huang,Kalaiselvi Palanisamy,Shih-Sheng Chang,I-Kuan Wang,Kang-Hsi Wu,Ping Chen,Ching-Tien Peng,Chi-Yuan Li

The Journal of biological chemistry 291:16576-85 PubMed27288406

2016

Combining Single Strand Oligodeoxynucleotides and CRISPR/Cas9 to Correct Gene Mutations in β-Thalassemia-induced Pluripotent Stem Cells.

Applications

Species

Human

Xiaohua Niu,Wenyin He,Bing Song,Zhanhui Ou,Di Fan,Yuchang Chen,Yong Fan,Xiaofang Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-fetal hemoglobin antibody [EPR9708(B)]

Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (AB137096)

Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (AB137096)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise