Anti-Fetuin A antibody [EPR9291] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin A antibody [EPR9291] - BSA and Azide free (AB226164)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137125).

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Fetuin A with ab137125 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : weak staining on blood vessel of human skeletal muscle, no staining in myocytes.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin A antibody [EPR9291] - BSA and Azide free (AB226164)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137125).

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Fetuin A with ab137125 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human kidney (PMID : 38577269).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin A antibody [EPR9291] - BSA and Azide free (AB226164)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137125).

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Fetuin A with ab137125 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human liver cancer (PMID : 26151126).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Unknown

Western blot - Anti-Fetuin A antibody [EPR9291] - BSA and Azide free (AB226164)

This data was developed using ab137125, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Fetuin A antibody [EPR9291] (<a href='/en-us/products/primary-antibodies/fetuin-a-antibody-epr9291-ab137125'>ab137125</a>) at 1/1000 dilution

Lane 1:

HepG2 lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Lane 3:

Human plasma lysate at 10 µg

Lane 4:

Human serum lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 39 kDa

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