AB309359

Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal FEZ1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

View Alternative Names

Fasciculation and elongation protein zeta-1, Zygin I, Zygin-1, FEZ1

4 Images

ELISA - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

ELISA

Supplier Data

ELISA - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

This data was developed using ab309358, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab309358 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human Fasciculation and elongation protein zeta-1.

Antigen concentration : 1000 ng/ml

Flow Cytometry (Intracellular) - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

This data was developed using ab309358, the same antibody clone in a different buffer formulation. Flow cytometric analysis of mouse primary neuron cells labelling Fez1 with ab309358 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

This data was developed using ab309358, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : heart (PMID : 33395696), spleen(PMID : 15735004). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-Fez1 antibody [EPR28403-51] (<a href='/en-us/products/primary-antibodies/fez1-antibody-epr28403-51-ab309358'>ab309358</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat cerebellum tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Observed band size: 65 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

This data was developed using ab309358, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen(PMID : 15735004). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 125 seconds

All lanes:

Western blot - Anti-Fez1 antibody [EPR28403-51] (<a href='/en-us/products/primary-antibodies/fez1-antibody-epr28403-51-ab309358'>ab309358</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Observed band size: 65 kDa

false

Exposure time: 125s

Unconjugated

Anti-Fez1 antibody [EPR28403-51]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28403-51

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

Flow Cyt (Intra), WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for human FC-Intra

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.

See full target information FEZ1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free

ELISA - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

Flow Cytometry (Intracellular) - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Product promise