Rabbit Recombinant Monoclonal FEZ1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359), expandable thumbnail](https://content.abcam.com/products/images/fez1-antibody-epr28403-51-bsa-and-azide-free-ab309359--flow-cytometry-intracellular-img415972.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)")
![ELISA - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359), expandable thumbnail](https://content.abcam.com/products/images/fez1-antibody-epr28403-51-bsa-and-azide-free-ab309359--elisa-img415973.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)")
![Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359), expandable thumbnail](https://content.abcam.com/products/images/fez1-antibody-epr28403-51-bsa-and-azide-free-ab309359--western-blot-img410169.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)")
![Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359), expandable thumbnail](https://content.abcam.com/products/images/fez1-antibody-epr28403-51-bsa-and-azide-free-ab309359--western-blot-img410168.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (AB309359)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | I-ELISA | IHC-P | ICC/IF | IP | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Expected | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.
Alternative names
Fasciculation and elongation protein zeta-1, Zygin I, Zygin-1, FEZ1
Recommended products
Rabbit Recombinant Monoclonal FEZ1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28403-51
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for human FC-Intra
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry (Intracellular) - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359), expandable thumbnail](https://content.abcam.com/products/images/fez1-antibody-epr28403-51-bsa-and-azide-free-ab309359--flow-cytometry-intracellular-img415972.jpg "Flow Cytometry (Intracellular) - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359)")
Flow Cytometry (Intracellular) - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359)
This data was developed using Anti-Fez1 antibody [EPR28403-51] ab309358, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of mouse primary neuron cells labelling Fez1 with Anti-Fez1 antibody [EPR28403-51] ab309358 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![ELISA - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359), expandable thumbnail](https://content.abcam.com/products/images/fez1-antibody-epr28403-51-bsa-and-azide-free-ab309359--elisa-img415973.jpg "ELISA - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359)")
ELISA - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359)
This data was developed using Anti-Fez1 antibody [EPR28403-51] ab309358, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of abab309358 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human Fasciculation and elongation protein zeta-1.
Antigen concentration: 1000 ng/ml![Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359), expandable thumbnail](https://content.abcam.com/products/images/fez1-antibody-epr28403-51-bsa-and-azide-free-ab309359--western-blot-img410169.jpg "Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359)")
Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359)
This data was developed using Anti-Fez1 antibody [EPR28403-51] ab309358, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: heart (PMID:33395696), spleen(PMID:15735004).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-Fez1 antibody [EPR28403-51] (Anti-Fez1 antibody [EPR28403-51] ab309358) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat cerebellum tissue lysate at 20 µg
Lane 6: Rat heart tissue lysate at 20 µg
Lane 7: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Observed band size: 65 kDa
Exposure time: 180s
![Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359), expandable thumbnail](https://content.abcam.com/products/images/fez1-antibody-epr28403-51-bsa-and-azide-free-ab309359--western-blot-img410168.jpg "Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359)")
Western blot - Anti-Fez1 antibody [EPR28403-51] - BSA and Azide free (ab309359)
This data was developed using Anti-Fez1 antibody [EPR28403-51] ab309358, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen(PMID:15735004).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 125 secondsAll lanes: Western blot - Anti-Fez1 antibody [EPR28403-51] (Anti-Fez1 antibody [EPR28403-51] ab309358) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Human spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/100000 dilution
Observed band size: 65 kDa
Exposure time: 125s
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