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AB185968

Anti-FGD2 antibody [EPR15306] - N-terminal

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(1 Publication)

Rabbit Recombinant Monoclonal FGD2 antibody. N-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ZFYVE4, FGD2, Zinc finger FYVE domain-containing protein 4

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-FGD2 antibody [EPR15306] - N-terminal (AB185968)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FGD2 antibody [EPR15306] - N-terminal (AB185968)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling FGD2 with ab185968 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGD2 antibody [EPR15306] - N-terminal (AB185968)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGD2 antibody [EPR15306] - N-terminal (AB185968)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FGD2 with ab185968 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FGD2 antibody [EPR15306] - N-terminal (AB185968)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FGD2 antibody [EPR15306] - N-terminal (AB185968)

Immunofluorescent analysis of acetone-fixed Raji cells labeling FGD2 with ab185968 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Western blot - Anti-FGD2 antibody [EPR15306] - N-terminal (AB185968)
  • WB

Supplier Data

Western blot - Anti-FGD2 antibody [EPR15306] - N-terminal (AB185968)

All lanes:

Western blot - Anti-FGD2 antibody [EPR15306] - N-terminal (ab185968) at 1/10000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human lymph node tissue lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

  • Carrier free

    Anti-FGD2 antibody [EPR15306] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15306

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGD2 also known as FYVE RhoGEF and PH domain-containing protein 6 (FGD1) is a protein with a mass of about 92 kDa. FGD2 functions mechanically as a guanine nucleotide exchange factor (GEF) that activates Rho GTPases. This protein is mostly expressed in hematopoietic tissues including the spleen and lymph nodes. FGD2 facilitates actin cytoskeleton organization which is essential for various cellular processes.
Biological function summary

FGD2 has a role in cellular signaling and cytoskeletal dynamics. It regulates the activation of Rho GTPases such as Cdc42 a small signaling G protein. FGD2 does not function within a known protein complex but partners with other signaling molecules to influence cell shape and movement. This biological activity affects how cells respond to external stimuli.

Pathways

FGD2 is involved in regulating cytoskeleton organization and cell signaling pathways. In the context of the Wnt signaling pathway FGD2 interacts with proteins that modulate cytoskeletal rearrangement such as Rac1 and Cdc42. These interactions suggest FGD2 influences pathways critical for cell division and differentiation.

Abnormalities in FGD2 are related to immune system disorders and some cancers. Dysregulated FGD2 expression associates especially with Hodgkin's lymphoma where it affects actin polymerization and cell migration. Additionally alterations in FGD2 interact with beta-catenin in Wnt signaling pathways related to tumorigenesis. Understanding FGD2's role can provide insights into therapeutic strategies for disorders linked to actin cytoskeletal defects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates JNK1 via CDC42 but not RAC1. Binds to phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate (By similarity).
See full target information FGD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biomedicine 5:31 PubMed39117956

2024

Inhibition of Sesn2 has negative regulatory effects on the myogenic differentiation of C2C12 myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zubiao Song,Qing Lin,Jiahui Liang,Weixi Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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