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AB97785

Anti-FGD4 antibody

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(5 Publications)

Rabbit Polyclonal FGD4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FGD4 aa 1-300.

View Alternative Names

FRABP, ZFYVE6, FGD4, Actin filament-binding protein frabin, FGD1-related F-actin-binding protein, Zinc finger FYVE domain-containing protein 6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGD4 antibody (AB97785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGD4 antibody (AB97785)

ab97785 at 1/100 dilution staining FGD4 in SG xenograft by Immunohistochemistry, Paraffin-embedded tissue.

Immunocytochemistry/ Immunofluorescence - Anti-FGD4 antibody (AB97785)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FGD4 antibody (AB97785)

ab97785 at 1/200 dilution staining FGD4 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.

Western blot - Anti-FGD4 antibody (AB97785)
  • WB

Unknown

Western blot - Anti-FGD4 antibody (AB97785)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-FGD4 antibody (ab97785) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 87 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FGD4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q96M96

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGD4 also known as Frabin acts as a guanine nucleotide exchange factor (GEF) that specifically activates Cdc42 a small GTPase. The FGD4 protein typically has a molecular mass of approximately 67 kDa. It is mainly expressed in tissues such as peripheral nerve brain and kidney. The protein contains several domains including a Dbl homology (DH) domain and a pleckstrin homology (PH) domain which are instrumental in its GEF activity.
Biological function summary

The FGD4 protein is essential in regulating the actin cytoskeleton dynamics and cell shape changes. FGD4 actively participates in cellular processes like migration invasion and polarization by facilitating actin filament assembly. Although not part of a larger protein complex its function in the actin cytoskeleton impacts various downstream signaling pathways influencing cell movement and morphology.

Pathways

FGD4 plays a significant role in the Rho GTPase cycle which regulates actin cytoskeleton remodeling. Within this pathway FGD4 activates Cdc42 leading to interactions with proteins like N-WASP that mediate actin polymerization through the Arp2/3 complex. Additionally FGD4 has associations with the PI3K/Akt signaling pathway influencing cell growth and survival decisions linking indirectly with proteins like PAK1 and PI3K.

FGD4 mutations have a known association with Charcot-Marie-Tooth disease type 4H a hereditary neurological disorder. The protein's disruption affects peripheral nerve function showing a direct link to demyelinating processes. Moreover connections exist between FGD4 dysregulation and certain cancer types such as breast cancer where aberrant expression may contribute to invasive cell behavior. In these contexts FGD4 interacts with factors like Cdc42 and Rac1 reflecting its role in cytoskeletal and cellular dynamics essential for disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8 (By similarity).
See full target information FGD4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 13:90 PubMed32772928

2020

MiR-23a induced the activation of CDC42/PAK1 pathway and cell cycle arrest in human cov434 cells by targeting FGD4.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Lin,Huijuan Huang,Liheng Lin,Weiwei Li,Jianfen Huang

Pancreas 48:1307-1311 PubMed31688594

2019

FGD4 (Frabin) Overexpression in Pancreatic Neuroendocrine Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Shahid,Tarra B George,James Saller,Mohammad Haija,Zena Sayegh,David Boulware,Jonathan Strosberg,Ratna Chakrabarti,Domenico Coppola

Proceedings of the National Academy of Sciences of 114:3915-3920 PubMed28348208

2017

MYO6 is targeted by virulence effectors to trigger PI3-kinase signaling and pathogen invasion into host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew B E Brooks,Daniel Humphreys,Vikash Singh,Anthony C Davidson,Susan D Arden,Folma Buss,Vassilis Koronakis

Journal of immunology (Baltimore, Md. : 1950) 198:1887-1899 PubMed28130497

2017

Mechanisms of Impaired Neutrophil Migration by MicroRNAs in Myelodysplastic Syndromes.

Applications

Unspecified application

Species

Unspecified reactive species

Meiwan Cao,Yayoi Shikama,Hideo Kimura,Hideyoshi Noji,Kazuhiko Ikeda,Tomoyuki Ono,Kazuei Ogawa,Yasuchika Takeishi,Junko Kimura

Molecular cancer 13:1 PubMed24387052

2014

MicroRNA expressions associated with progression of prostate cancer cells to antiandrogen therapy resistance.

Applications

WB

Species

Human

Richard Ottman,Camha Nguyen,Robert Lorch,Ratna Chakrabarti
View all publications
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