JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB319994

Anti-FGF-15 antibody [EPR29018-80]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal FGF-15 antibody. Suitable for WB and reacts with Transfected cell lysate - Mouse, Mouse samples.

View Alternative Names

Fibroblast growth factor 15, FGF-15, Fgf15

3 Images
Western blot - Anti-FGF-15 antibody [EPR29018-80] (AB319994)
  • WB

Supplier Data

Western blot - Anti-FGF-15 antibody [EPR29018-80] (AB319994)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-FGF-15 antibody [EPR29018-80] (ab319994) at 1/1000 dilution

All lanes:

Mouse ileum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 21 kDa

false

Exposure time: 81s

Western blot - Anti-FGF-15 antibody [EPR29018-80] (AB319994)
  • WB

Supplier Data

Western blot - Anti-FGF-15 antibody [EPR29018-80] (AB319994)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : mouse colon, mouse liver (PMID : 16213224).

The identity of the bands higher than 30 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FGF-15 antibody [EPR29018-80] (ab319994) at 1/1000 dilution

Lane 1:

Mouse ileum tissue lysate at 30 µg

Lane 2:

Mouse E12 embryo tissue lysate at 30 µg

Lane 3:

Mouse colon tissue lysate at 30 µg

Lane 4:

Mouse liver tissue lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 21 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-FGF-15 antibody [EPR29018-80] (AB319994)
  • WB

Supplier Data

Western blot - Anti-FGF-15 antibody [EPR29018-80] (AB319994)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-FGF-15 antibody [EPR29018-80] (ab319994) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 7 µg

Lane 2:

293T cells transfected with a mouse Fgf15 expression vector containing a myc-His-tag®, whole cell lysate at 7 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 27 kDa,36 kDa

false

Exposure time: 26s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29018-80

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
style
left-column
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF-15 also known as fibroblast growth factor 15 is a protein with a significant role in various physiological processes. This protein belongs to the fibroblast growth factor (FGF) family and has a molecular mass of approximately 25 kDa. FGF-15 is predominantly expressed in the intestines particularly in the ileum. Its main mechanical function involves the modulation of bile acid synthesis which occurs through its actions on specific receptor targets.
Biological function summary

FGF-15 plays a critical role in liver function and metabolism. It acts as a regulatory factor in bile acid homeostasis and glucose metabolism ensuring the balance and maintenance within the body. FGF-15 does not primarily function as part of a protein complex but it exerts its influence by binding to the fibroblast growth factor receptor 4 (FGFR4) associated with the β-Klotho co-receptor eliciting a signaling cascade important for maintaining metabolic homeostasis.

Pathways

FGF-15 engages in the enterohepatic circulation of bile acids and has a pivotal role in the suppression of bile acid synthesis. One of the key pathways involves fibroblast growth factor receptor signaling pathway. In this capacity FGF-15 works in conjunction with proteins such as FGFR4 and β-Klotho forming a signaling axis that influences the expression of cytochrome P450 family 7 subfamily A member 1 (CYP7A1) an important enzyme in bile acid biosynthesis.

FGF-15 is closely associated with metabolic conditions particularly liver diseases such as non-alcoholic fatty liver disease (NAFLD). Elevated levels of protein expression or dysregulation of its pathway may contribute to the development or progression of such disorders. Additionally abnormalities in the FGF-15 signaling can also impact glucose homeostasis linking it to diabetes. In these contexts FGF-15 works closely with FGFR4 and β-Klotho both important in the therapeutic targeting of these metabolic diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression.
See full target information Fgf15
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com