AB56326

Anti-FGF 23 antibody

Name: Anti-FGF 23 antibody (ab56326) | Abcam
Brand: Abcam Limited
SKU: AB56326
Price: 525 USD
Availability: InStock

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(14 Publications)

Goat Polyclonal FGF 23 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human FGF23 aa 150-200.

View Alternative Names

HYPF, UNQ3027/PRO9828, FGF23, Fibroblast growth factor 23, FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor

2 Images

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Western blot - Anti-FGF 23 antibody (AB56326)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-FGF 23 antibody (ab56326) at 0.5 µg/mL

All lanes:

Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer)

Predicted band size: 28 kDa

Observed band size: 27 kDa,37 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-FGF 23 antibody (AB56326)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FGF 23 antibody (AB56326)

ICC/IF image of ab56326 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56326, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human FGF23 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q9GZV9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fibroblast Growth Factor 23 also known as FGF 23 is a hormone-like protein that plays a role in phosphate and vitamin D metabolism. It is a member of the fibroblast growth factor family and consists of about 251 amino acids with a mass of approximately 32 kDa. FGF 23 is predominantly expressed in bone cells especially osteocytes but it also appears in other tissues such as the thymus and brain. An important tool for quantitative measurement of FGF 23 levels is the FGF 23 ELISA kit which assists in various research and clinical settings.

Biological function summary

FGF 23 plays an important role in regulating phosphate balance in the body. It impacts the function of kidneys by decreasing the reabsorption of phosphate and suppressing the formation of active vitamin D thereby affecting calcium regulation as well. FGF 23 does not operate alone but interacts with co-receptors such as Klotho to activate its receptor complex. The protein functions as part of a larger system that fine-tunes the balance of minerals important for maintaining bone health and metabolic functions.

Pathways

FGF 23 is part of the bone-mineral metabolism axis and is integral to the phosphate regulation pathway. It works closely with proteins like Klotho and FGFR1 and their interaction ensures proper signaling required for maintaining serum phosphate levels. FGF 23 controls vitamin D metabolism by regulating the expression of 1-alpha-hydroxylase and 24-hydroxylase influencing the activity of the related vitamin D endocrine system. Its actions help stabilize phosphate and vitamin D status which are both essential for skeletal and mineral homeostasis.

FGF 23 has a significant connection to conditions such as Hypophosphatemic Rickets and Chronic Kidney Disease (CKD). In these cases FGF 23 levels may become dysregulated leading to abnormal phosphate metabolism and bone mineralization defects. In Hypophosphatemic Rickets altered expression of FGF 23 causes excessive phosphate wasting whereas in CKD increased FGF 23 levels are associated with cardiovascular disease risk and deteriorating kidney function. Klotho as a coreceptor for FGF 23 also influences these pathological conditions highlighting its contribution to phosphate-related disorder mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of phosphate homeostasis (PubMed : 11062477). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (PubMed : 11409890). Up-regulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (PubMed : 15040831). Negatively regulates osteoblast differentiation and matrix mineralization (PubMed : 18282132).

See full target information FGF23

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Medical sciences (Basel, Switzerland) 13: PubMed40981193

2025

Mast Cell Association with the Microenvironment of a Phosphaturic Mesenchymal Tumour Secreting Fibroblast Growth Factor 23.

Applications

Unspecified application

Species

Unspecified reactive species

Andrey Kostin,Alexei Lyundup,Alexander Alekhnovich,Aleksandra Prikhodko,Olga Patsap,Sofia Gronskaia,Zhanna Belaya,Olga Lesnyak,Galina Melnichenko,Natalia Mokrysheva,Igor Buchwalow,Markus Tiemann,Dmitrii Atiakshin

Cells 13: PubMed39329699

2024

FGF23 and Cell Stress in SaOS-2 Cells-A Model Reflecting X-Linked Hypophosphatemia Dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Lisanne Brueck,Sascha Roocke,Veronika Matschke,Annette Richter-Unruh,Katrin Marcus-Alic,Carsten Theiss,Sarah Stahlke

Frontiers in endocrinology 15:1296886 PubMed38828417

2024

Targeted silencing of in a human model of osteoprogenitor cells results in the deregulation of the osteogenic differentiation program.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Marta Elli,Deborah Mattinzoli,Masami Ikehata,Francesca Bagnaresi,Maria A Maffini,Giulia Del Sindaco,Angela Pagnano,Camilla Lucca,Piergiorgio Messa,Maura Arosio,Giuseppe Castellano,Carlo M Alfieri,Giovanna Mantovani

International journal of molecular sciences 24: PubMed38069366

2023

Cardioprotective and Antifibrotic Effects of Low-Dose Renin-Angiotensin-Aldosterone System Inhibitors in Type 1 Diabetic Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Dora B Balogh,Agnes Molnar,Arianna Degi,Akos Toth,Lilla Lenart,Adar Saeed,Adrienn Barczi,Attila J Szabo,Laszlo J Wagner,Gyorgy Reusz,Andrea Fekete

Journal of orthopaedic surgery and research 18:12 PubMed36604721

2023

FGF23 promotes proliferation, migration and invasion by regulating miR-340-5p in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lun Fang,Zhongzhe Li,Beilei Yu,Lu Zhou

The European journal of neuroscience 54:5261-5271 PubMed34184338

2021

Localization of fibroblast growth factor 23 protein in the rat hypothalamus.

Applications

Unspecified application

Species

Unspecified reactive species

Stan R Ursem,Charlene Diepenbroek,Vesna Bacic,Unga A Unmehopa,Leslie Eggels,Clarissa M Maya-Monteiro,Annemieke C Heijboer,Susanne E la Fleur

The Journal of clinical endocrinology and metaboli : PubMed33462615

2021

Paraneoplastic Secretion of Multiple Phosphatonins From a Deep Fibrous Histiocytoma Causing Oncogenic Osteomalacia.

Applications

Unspecified application

Species

Unspecified reactive species

Melvin Khee Shing Leow,Shaillay Dogra,Xiaojia Ge,Khoon Leong Chuah,Huiling Liew,Kelvin Siu Hoong Loke,Craig McFarlane

Biochemical and biophysical research communication 514:618-624 PubMed31076107

2019

Mg-Zn-Mn alloy extract induces the angiogenesis of human umbilical vein endothelial cells via FGF/FGFR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Li,Qi Yuan,Kun Yu,Tao Xiao,Lihong Liu,Yilong Dai,Liang Xiong,Boyu Zhang,Aoyu Li

Kidney international 92:165-178 PubMed28341272

2017

Kidney fibroblast growth factor 23 does not contribute to elevation of its circulating levels in uremia.

Applications

Unspecified application

Species

Unspecified reactive species

Maria L Mace,Eva Gravesen,Anders Nordholm,Jacob Hofman-Bang,Thomas Secher,Klaus Olgaard,Ewa Lewin

Stem cell research & therapy 8:71 PubMed28320453

2017

Dysregulated systemic lymphocytes affect the balance of osteogenic/adipogenic differentiation of bone mesenchymal stem cells after local irradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoya Xu,Ruixia Li,Yi Zhou,Qiong Zou,Qiaoling Ding,Jinfeng Wang,Weifang Jin,Guoqiang Hua,Jianjun Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF 23 antibody

Western blot - Anti-FGF 23 antibody (AB56326)

Immunocytochemistry/ Immunofluorescence - Anti-FGF 23 antibody (AB56326)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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