AB207321

Anti-FGF1 antibody [EPR19989]

RabMAb
Recombinant
What is this?

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(15 Publications)

Rabbit Recombinant Monoclonal FGF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 15 publications.

View Alternative Names

FGFA, FGF1, Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, aFGF, ECGF, HBGF-1

2 Images

Supplier Data

Western blot - Anti-FGF1 antibody [EPR19989] (AB207321)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 1 second; Lane 2/4 : 5 seconds; Lane 3 : 3 seconds.

The observed molecular weight is consistent with the literature (PMID : 16091423).

All lanes:

Western blot - Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 17 kDa

Observed band size: 16 kDa,18 kDa

false

Supplier Data

Western blot - Anti-FGF1 antibody [EPR19989] (AB207321)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1/2/5 : 3 minutes; Lane 3 : 10 seconds; Lane 4 : 15 seconds.

The observed molecular weight is consistent with the literature (PMID : 16091423).

All lanes:

Western blot - Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution

Lane 1:

Human glioma lysate at 10 µg

Lane 2:

Mouse spinal cord lysate at 10 µg

Lane 3:

Mouse heart lysate at 10 µg

Lane 4:

Mouse kidney lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Secondary

Lane 1:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Lanes 2 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 17 kDa

Observed band size: 16 kDa,18 kDa

false

Carrier free

Anti-FGF1 antibody [EPR19989] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19989

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF1 also known as acidic fibroblast growth factor (aFGF) is a member of the fibroblast growth factor family. This protein has a molecular mass of approximately 17 kDa. FGF1 is expressed in a variety of tissues such as the brain liver and heart and plays an essential role in cell growth development and repair. The FGF1 protein is sometimes studied using techniques such as FGF1 ELISA which helps in quantifying and understanding its expression levels and activity in different contexts.

Biological function summary

FGF1 is involved in numerous cellular processes beyond simple cell proliferation. It binds to fibroblast growth factor receptors (FGFRs) on the cell surface initiating a cascade of downstream signaling events. This protein does not act alone; it is often a part of larger protein complexes that modulate cellular processes such as angiogenesis and wound healing. FGF1 is also implicated in nerve cell differentiation and survival through FGFR-mediated pathways.

Pathways

The FGF1 protein is integrally involved in the MAPK/ERK signaling pathway a critical pathway that regulates cell division growth and differentiation. Through this pathway FGF1 interacts with various proteins including Ras Raf and ERK to propagate the signal necessary for mitogenic and survival responses. FGF1 is also a player in the phosphoinositide 3-kinase (PI3K) pathway which helps control cellular responses to stress and metabolic regulation showing its role in cellular homeostasis.

FGF1 is associated with conditions such as cancer and cardiovascular diseases. The deregulation of FGF1 expression or its signaling pathways can lead to tumor development and progression with FGF1 often interacting with other proteins like the basic fibroblast growth factor (bFGF) in these contexts. In cardiovascular diseases altered FGF1 signaling might contribute to atherosclerosis and cardiac repair processes where its interaction with other growth factors like vascular endothelial growth factor (VEGF) is significant.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV : ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 18441324, PubMed : 20422052). Can induce angiogenesis (PubMed : 23469107).

See full target information FGF1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:6623 PubMed39994211

2025

Regenerative effect of lyophilized dental follicle mesenchymal stem cells and platelet-rich fibrin in skin wounds in geriatric and young rats.

Applications

Unspecified application

Species

Unspecified reactive species

Osman Bulut,Deniz Genc,Çiğdem Elif Demirci,Leyla Tekin,Tolga Meriç Dümbek,Aziz Bülbül

Cell reports 43:114789 PubMed39325622

2024

Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yidan Xu,Ana B Alves-Wagner,Hitoshi Inada,Sepideh D Firouzjah,Shion Osana,Muhammad Subhan Amir,Royce H Conlin,Michael F Hirshman,Eva S Nozik,Laurie J Goodyear,Ryoichi Nagatomi,Joji Kusuyama

Journal of translational medicine 22:474 PubMed38764020

2024

Fibroblast growth factor pathway promotes glycolysis by activating LDHA and suppressing LDHB in a STAT1-dependent manner in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongkang Ye,Fukan Yang,Zhanhao Gu,Wenxuan Li,Yinjiao Yuan,Shaoqian Liu,Le Zhou,Bo Han,Ruinian Zheng,Zhengguo Cao

International journal of molecular sciences 25: PubMed38542224

2024

Mitochondrial Ribosomal Protein MRPS15 Is a Component of Cytosolic Ribosomes and Regulates Translation in Stressed Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Florian David,Emilie Roussel,Carine Froment,Tangra Draia-Nicolau,Françoise Pujol,Odile Burlet-Schiltz,Anthony K Henras,Eric Lacazette,Florent Morfoisse,Florence Tatin,Jean-Jacques Diaz,Frédéric Catez,Barbara Garmy-Susini,Anne-Catherine Prats

International journal of biological sciences 20:1669-1687 PubMed38481813

2024

Tubule-specific cyclin-dependent kinase 12 knockdown potentiates kidney injury through transcriptional elongation defects.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lin Zhang,Tao-Tao Tang,Wei-Jie Ni,Zhong-Tang Li,Liang-Yun-Zi Jiang,Yao Wang,Xuan Zhou,Jing-Yuan Cao,Qing Yin,Wei Jiang,Ya-Jie Zhao,Wei-Hua Gan,Ai-Qing Zhang,Zuo-Lin Li,Yi Wen,Lin-Li Lv,Bi-Cheng Liu,Bin Wang

Thrombosis journal 21:24 PubMed36890543

2023

LINC00659 exacerbates endothelial progenitor cell dysfunction in deep vein thrombosis of the lower extremities by activating DNMT3A-mediated FGF1 promoter methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Jie Qin

Nature communications 14:1235 PubMed36871047

2023

Fibroblast growth factor 18 alleviates stress-induced pathological cardiac hypertrophy in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Gen Chen,Ning An,Jingling Shen,Huinan Chen,Yunjie Chen,Jia Sun,Zhicheng Hu,Junhui Qiu,Cheng Jin,Shengqu He,Lin Mei,Yanru Sui,Wanqian Li,Peng Chen,Xueqiang Guan,Maoping Chu,Yang Wang,Litai Jin,Kwonseop Kim,Xiaokun Li,Weitao Cong,Xu Wang

Nature communications 14:344 PubMed36670126

2023

Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Pei,Li Ma,Junjun Jing,Jifan Feng,Yuan Yuan,Tingwei Guo,Xia Han,Thach-Vu Ho,Jie Lei,Jinzhi He,Mingyi Zhang,Jian-Fu Chen,Yang Chai

eLife 11: PubMed36546462

2022

Long non-coding RNA and paraspeckle components are translational regulators in hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Anne-Claire Godet,Emilie Roussel,Florian David,Fransky Hantelys,Florent Morfoisse,Joffrey Alves,Françoise Pujol,Isabelle Ader,Edouard Bertrand,Odile Burlet-Schiltz,Carine Froment,Anthony K Henras,Patrice Vitali,Eric Lacazette,Florence Tatin,Barbara Garmy-Susini,Anne-Catherine Prats

Brain sciences 12: PubMed35741678

2022

Binge-like Prenatal Ethanol Exposure Causes Impaired Cellular Differentiation in the Embryonic Forebrain and Synaptic and Behavioral Defects in Adult Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shivakumar Subbanna,Balapal S Basavarajappa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF1 antibody [EPR19989]

Western blot - Anti-FGF1 antibody [EPR19989] (AB207321)

Western blot - Anti-FGF1 antibody [EPR19989] (AB207321)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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