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Rabbit Recombinant Monoclonal FGF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

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Images

Western blot - Anti-FGF1 antibody [EPR19989] (AB207321), expandable thumbnail
  • Western blot - Anti-FGF1 antibody [EPR19989] (AB207321), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:18441324, PubMed:20422052). Can induce angiogenesis (PubMed:23469107).

Alternative names

FGFA, FGF1, Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, aFGF, ECGF, HBGF-1

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Rabbit Recombinant Monoclonal FGF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19989
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FGF1 also known as acidic fibroblast growth factor (aFGF) is a member of the fibroblast growth factor family. This protein has a molecular mass of approximately 17 kDa. FGF1 is expressed in a variety of tissues such as the brain liver and heart and plays an essential role in cell growth development and repair. The FGF1 protein is sometimes studied using techniques such as FGF1 ELISA which helps in quantifying and understanding its expression levels and activity in different contexts.

Biological function summary

FGF1 is involved in numerous cellular processes beyond simple cell proliferation. It binds to fibroblast growth factor receptors (FGFRs) on the cell surface initiating a cascade of downstream signaling events. This protein does not act alone; it is often a part of larger protein complexes that modulate cellular processes such as angiogenesis and wound healing. FGF1 is also implicated in nerve cell differentiation and survival through FGFR-mediated pathways.

Pathways

The FGF1 protein is integrally involved in the MAPK/ERK signaling pathway a critical pathway that regulates cell division growth and differentiation. Through this pathway FGF1 interacts with various proteins including Ras Raf and ERK to propagate the signal necessary for mitogenic and survival responses. FGF1 is also a player in the phosphoinositide 3-kinase (PI3K) pathway which helps control cellular responses to stress and metabolic regulation showing its role in cellular homeostasis.

Associated diseases and disorders

FGF1 is associated with conditions such as cancer and cardiovascular diseases. The deregulation of FGF1 expression or its signaling pathways can lead to tumor development and progression with FGF1 often interacting with other proteins like the basic fibroblast growth factor (bFGF) in these contexts. In cardiovascular diseases altered FGF1 signaling might contribute to atherosclerosis and cardiac repair processes where its interaction with other growth factors like vascular endothelial growth factor (VEGF) is significant.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-FGF1 antibody [EPR19989] (ab207321), expandable thumbnail

    Western blot - Anti-FGF1 antibody [EPR19989] (ab207321)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lane 2/4: 5 seconds; Lane 3: 3 seconds.

    The observed molecular weight is consistent with the literature (PMID: 16091423).

    All lanes: Western blot - Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution

    Lane 1: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Lane 3: Human fetal brain lysate at 10 µg

    Lane 4: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 17 kDa

    Observed band size: 16 kDa, 18 kDa

  • Western blot - Anti-FGF1 antibody [EPR19989] (ab207321), expandable thumbnail

    Western blot - Anti-FGF1 antibody [EPR19989] (ab207321)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2/5: 3 minutes; Lane 3: 10 seconds; Lane 4: 15 seconds.

    The observed molecular weight is consistent with the literature (PMID: 16091423).

    All lanes: Western blot - Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution

    Lane 1: Human glioma lysate at 10 µg

    Lane 2: Mouse spinal cord lysate at 10 µg

    Lane 3: Mouse heart lysate at 10 µg

    Lane 4: Mouse kidney lysate at 10 µg

    Lane 5: Rat heart lysate at 10 µg

    Secondary

    Lane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution

    Lanes 2 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 17 kDa

    Observed band size: 16 kDa, 18 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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