Anti-FGF13 antibody [EPR29561-52]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF13 antibody [EPR29561-52] (AB325168)

Immunohistochemical analysis of paraffin-embedded (A) RD (human muscle multinucleated spindle-shaped cell) cell pellet and (B) HepG2 (human hepatocellular carcinoma epithelial cell) cell pellet labeling FGF13 with ab325168 at 1/100 (5.14 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) RD cell pellet, no signal in (B) HepG2 cell pellet.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-FGF13 antibody [EPR29561-52] (AB325168)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HepG2.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 39773461).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-FGF13 antibody [EPR29561-52] (ab325168)

Lane 1:

RD (human muscle multinucleated spindle-shaped cell) whole cell lysate at 80 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,30 kDa,36 kDa

false

Exposure time: 48s

• WB

Lab

Western blot - Anti-FGF13 antibody [EPR29561-52] (AB325168)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab325168 was shown to bind specifically to FGF13. Target of interest was observed at 20-30 kDa in wild-type HEK-293 cell lysates (lane 1) with no signal observed at this size in FGF13 knockout cell line (lane 2) (lane 2, knockout cell line ab267284 / knockout cell lysate ab258871).

The identity of the higher MW band at approximately 150 kDa is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-FGF13 antibody [EPR29561-52] (ab325168)

Lane 1:

Wilde-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 80 µg

Lane 2:

FGF13 knockout HEK-293 whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,30 kDa,124 kDa

false

Exposure time: 180s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF13 antibody [EPR29561-52] (AB325168)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling FGF13 with ab325168 at 1/500 (1.028 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF13 antibody [EPR29561-52] (AB325168)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling FGF13 with ab325168 at 1/500 (1.028 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-FGF13 antibody [EPR29561-52] (AB325168)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human FGF14, FGF12 and FGF11.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 39773461).

In Western blot, Anti-His tag® antibody (ab15149) (1 : 1000)(18-35KDa).

All lanes:

Western blot - Anti-FGF13 antibody [EPR29561-52] (ab325168)

Lane 1:

His-tagged human FGF13 recombinant fragment at 10 ng

Lane 2:

His-tagged human FGF14 recombinant protein at 10 ng

Lane 3:

His-tagged human FGF12 recombinant protein at 10 ng

Lane 4:

His-tagged human FGF11 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20-30 kDa,18-35 kDa

false

Exposure time: 180s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF13 antibody [EPR29561-52] (AB325168)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling FGF13 with ab325168 at 1/500 (1.028 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human cerebrum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF13 antibody [EPR29561-52] (AB325168)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling FGF13 with ab325168 at 1/500 (1.028 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse cerebrum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins