AB186300

Anti-FGF13 antibody [N235/22]

Name: Anti-FGF13 antibody [N235/22] (ab186300) | Abcam
Brand: Abcam Limited
SKU: AB186300

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(3 Publications)

Mouse Monoclonal FGF13 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FGF13 aa 1-50.

View Alternative Names

FHF2, FGF13, Fibroblast growth factor 13, FGF-13, Fibroblast growth factor homologous factor 2, FHF-2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (AB186300)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (AB186300)

SK-N-BE (Human neuroblastoma cell line) cells stained for FGF13 using ab186300 at a dilution of 1/100 (1 hour at RT) in ICC/IF.

Secondary Antibody : Goat Anti-Mouse ATTO 488 at 1 : 100 for 1 hour at RT.

Cells were fixed with 4% formaldehyde for 15 minutes at RT.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (AB186300)

Immunofluorescent analysis of 4% Formaldehyde-fixed Neuroblastoma cell line (SK-N-BE) labeling FGF13 with ab186300 at 1/100 dilution. Secondary antibody : Goat Anti-Mouse ATTO 488 at 1/100 dilution. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) FGFA/FHFA (pan) Antibody. (D) Composite.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (AB186300)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling FGF13 with ab186300 at 1/50 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) FGFA/FHFA (pan) Antibody (D) Composite.

Supplier Data

Western blot - Anti-FGF13 antibody [N235/22] (AB186300)

Block : 2% BSA and 2% skimmed milk in 1X TBST.

Incubated with primary antibody for 16 hours at 4°C.

Incubated with secondary antibody for 1 hour at RT.

All lanes:

Western blot - Anti-FGF13 antibody [N235/22] (ab186300) at 1/200 dilution

All lanes:

Rat brain membrane lysate at 15 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/1000 dilution

Predicted band size: 28 kDa

true

Exposure time: 6min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N235/22

Isotype

IgG2b

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human FGF13 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q92913

Specificity

ab186300 will not recognize isoforms 2, 3, 4 or 5 of human FGF13. It cross-reacts with FGF12 and FGF14. It has 100% identity with human, mouse and rat FGF12; 94% identity with human, mouse and rat FGF14; 81% identity to human and mouse FGF11.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

1μg/mL of ab186300 was sufficient for detection of FGF13 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

The clone number has been updated from S235-22 to N235/22, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF13 also known as Fibroblast Growth Factor 13 is a member of the FGF family and plays a significant role in cellular processes. It has an approximate molecular mass of 29 kDa. You often find FGF13 expressed in the brain and heart indicating its involvement in the physiology of these organs. FGF13 does not function as a traditional growth factor through receptors but instead operates intracellularly engaging directly with cellular components.

Biological function summary

FGF13 plays important roles in regulating neuronal development and intracellular signaling pathways. It forms complexes with other proteins modulating their activities within cells. FGF13 is instrumental in neuronal outgrowth and synaptic plasticity essential functions for maintaining a healthy nervous system. Researchers identify FGF13 as important for proper neuronal positioning and survival.

Pathways

FGF13 is intricately involved in signaling mechanisms associated with ion channel regulation and mitogen-activated protein kinase (MAPK) pathway. It interacts with proteins such as kinases and other ion channel components to fine-tune cellular responses to extracellular stimuli. FGF13 by modulating these pathways assists in the precise control of cellular growth differentiation and adaptation.

FGF13 presents a significant connection to neurodevelopmental and cardiac disorders. Mutations in FGF13 or its altered expression levels have links to disorders such as epilepsy and cardiac arrhythmia. Through these diseases FGF13 often interacts with proteins like ion channels affecting their function and stability. These interactions highlight the potential of FGF13 as a target for therapeutic interventions in related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules (By similarity). Through its action on microtubules, may participate in the refinement of axons by negatively regulating axonal and leading processes branching (By similarity). Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus (By similarity). Regulates voltage-gated sodium channel transport and function (PubMed : 15282281, PubMed : 33245860, PubMed : 36696443). May also play a role in MAPK signaling (By similarity). Required for the development of axonal initial segment-targeting inhibitory GABAergic synapses made by chandelier neurons (By similarity).

See full target information FGF13

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 18:1347-1353 PubMed36453422

2022

Overexpression of fibroblast growth factor 13 ameliorates amyloid-β-induced neuronal damage.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Meng Li,Lan Xiao,Ting Zhang,Dan Ren,Hong Zhu

Analytical cellular pathology (Amsterdam) 2021:5565671 PubMed33628710

2021

Endoplasmic Reticulum Stress Is Involved in Glucocorticoid-Induced Apoptosis in PC12 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shanyong Yi,Weibo Shi,Min Zuo,Songjun Wang,Rufei Ma,Haitao Bi,Bin Cong,Yingmin Li

Molecular medicine reports 23: PubMed33495804

2021

lncRNA LINC00963 downregulation regulates colorectal cancer tumorigenesis and progression via the miR‑10b/FGF13 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujin Wu,Longling Cong,Wenjian Chen,Xuechuan Wang,Fanghua Qiu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF13 antibody [N235/22]

Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (AB186300)

Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (AB186300)

Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (AB186300)

Western blot - Anti-FGF13 antibody [N235/22] (AB186300)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise