Rabbit Recombinant Monoclonal FGF2 antibody. Carrier free. Suitable for IP, WB, sELISA, IHC-P and reacts with Human, Synthetic peptide - Human samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--immunoprecipitation-img4296.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86883.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134213.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91927.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354)")
![Sandwich ELISA - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--sandwich-elisa-img415529.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-FGF2 antibody [EP1735] - BSA and Azide free (AB246354)")
Publications
Filter by
- PloS one 17:e02647752022Four in one-Combination therapy using live Lactococcus lactis expressing three therapeutic proteins for the treatment of chronic non-healing wounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJere Kurkipuro,Igor Mierau,Thomas Wirth,Haritha Samaranayake,Wesley Smith,Hanna-Riikka Kärkkäinen,Mirka Tikkanen,Juha YrjänheikkiPubMed 35226700
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | sELISA | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested |
| Synthetic peptide - Human | Not recommended | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes For sandwich ELISA use this antibody as Detection at 0.5 ug/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:28302677, PubMed:8663044). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
Alternative names
FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2
Recommended products
Rabbit Recombinant Monoclonal FGF2 antibody. Carrier free. Suitable for IP, WB, sELISA, IHC-P and reacts with Human, Synthetic peptide - Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1735
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab246354 is the carrier-free version of Anti-FGF2 antibody [EP1735] ab92337.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.
- Biological function summary
Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.
- Pathways
FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.
- Associated diseases and disorders
Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--immunoprecipitation-img4296.jpg "Immunoprecipitation - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)")
Immunoprecipitation - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
Anti-FGF2 antibody [EP1735] ab92337 (Purified) at 1:70 dilution (2 μg) immunoprecipitating FGF2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2 (+): Anti-FGF2 antibody [EP1735] ab92337 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FGF2 antibody [EP1735] ab92337 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FGF2 antibody [EP1735] ab92337)
Anti-FGF2 antibody [EP1735] ab92337 recoginzes 3 isoforms of FGF2All lanes: Immunoprecipitation - Anti-FGF2 antibody [EP1735] (Anti-FGF2 antibody [EP1735] ab92337)
Predicted band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86883.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FGF2 with Purified Anti-FGF2 antibody [EP1735] ab92337 at 1:2400 dilution (0.51 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FGF2 antibody [EP1735] ab92337)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134213.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling FGF2 with purified Anti-FGF2 antibody [EP1735] ab92337 at 1/1000 dilution (1.3 ?g/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91927.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling FGF2 with purified Anti-FGF2 antibody [EP1735] ab92337 at 1/1000 dilution (1.3 ?g/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Sandwich ELISA - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354), expandable thumbnail](https://content.abcam.com/products/images/fgf2-antibody-ep1735-bsa-and-azide-free-ab246354--sandwich-elisa-img415529.jpg "Sandwich ELISA - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)")
Sandwich ELISA - Anti-FGF2 antibody [EP1735] - BSA and Azide free (ab246354)
Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (Recombinant human FGF2 protein (Active) ab9596)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1μg/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (Anti-FGF2 antibody [EP1735] ab92337) at 0.5μg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-FGF2 antibody [EP1735] ab92337).
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