Anti-FGF2 antibody [EPR20145-219]

8 product images

• 

  Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Exposure time: Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 5 seconds.

  The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).

  All lanes: Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/1000 dilution

  Lane 1: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

  Lane 2: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

  Lane 3: SK-OV-3 (Human ovarian cancer cell line) whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 30 kDa

  Observed band size: 18 kDa, 22 kDa, 24 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
  

  Confocal image showing nuclear and weak cytoplasmic staining on U-87 MG cell line.

  The nuclear counterstain is DAPI (blue).

  Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

• 

  Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/10000 dilution

  All lanes: Recombinant human FGF2 active protein (aa143-288) at 0.01 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 30 kDa

  Observed band size: 15 kDa

  Exposure time: 1s

• 

  Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).

  All lanes: Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/1000 dilution

  Lane 1: Human fetal kidney lysate at 10 µg

  Lane 2: Human prostate lysate at 10 µg

  Lane 3: Human fetal heart lysate at 10 µg

  Lane 4: Human testis lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

  Predicted band size: 30 kDa

  Observed band size: 18 kDa, 22 kDa, 24 kDa

  Exposure time: 3min

• 

  Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  FGF2 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab208687 at 1/30 dilution.
  

  Western blot was performed from the immunoprecipitate using ab208687 at 1/500 dilution.

  VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

  Lane 1: K562 whole cell lysate 10μg (Input).

  Lane 2: ab208687 IP in K562 whole cell lysate.

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab208687 in K562 whole cell lysate.

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 1 second.

  All lanes: Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  Predicted band size: 30 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
  

  Confocal image showing nuclear weak cytoplasmic staining on SK-OV-3 cell line.

  The nuclear counterstain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

• 

  Indirect ELISA - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  ELISA analysis of Mouse FGF2 recombinant protein at 1000 ng/ml with ab208687. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

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  Flow Cytometry (Intracellular) - Anti-FGF2 antibody [EPR20145-219] (ab208687)

  Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling FGF2 with ab208687 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.