AB321990

Anti-FGF2 antibody [EPR29019-77]

BOND RX™ Validated
20ul selling size
Recombinant
RabMAb
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(1 Publication)

Anti-FGF2 antibody [EPR29019-77] is a Rabbit Monoclonal antibody that is used in FGF2 IHC-P, Western Blot. Suitable for Mouse samples.

FGF2/FGF basic is a key growth factor involved in autocrine and paracrine oncogenic signalling that plays a role in angiogenesis and migration. FGFR inhibitors and FGF ligand traps are being explored in the clinic to treat multiple cancer types including breast, gastric and lung cancers. FGF signalling sustains prostate cancer proliferation in the absence of androgen receptor signalling.

View Alternative Names

Fgf-2, Fgf2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemical analysis of paraffin-embedded Mouse head and neck neuroendocrine carcinoma tissue labeling FGF2 with ab321990 at 1/2000 (0.255 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on stoma of mouse head and neck neuroendocrine carcinoma. The section was incubated with ab321990 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling FGF2 with ab321990 at 1/2000 (0.255 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on stoma of mouse colon. The section was incubated with ab321990 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling FGF2 with ab321990 at 1/2000 (0.255 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on Sertoli cells and Leydig cells of mouse testis (PMID : 16338792). The section was incubated with ab321990 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling FGF2 with ab321990 at 1/2000 (0.255 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on stoma of mouse lung (PMID : 8590811). The section was incubated with ab321990 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemical analysis of paraffin-embedded (A) MEF (mouse embryo fibroblast) cell pellet (B) Neuro-2a (mouse neuroblastoma neuroblast) cell pellet (C) B16-F10 (mouse skin melanoma cell) cell pellet tissue labeling FGF2 with ab321990 at 1/2000 (0.255 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on (A) MEF (mouse embryo fibroblast) cell pellet negative staining on (B) Neuro-2a (mouse neuroblastoma neuroblast) cell pellet and (C) B16-F10 (mouse skin melanoma cell) cell pellet. The section was incubated with ab321990 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

Supplier Data

Western blot - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a, B16-F10.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FGF2 antibody [EPR29019-77] (ab321990) at 1/1000 dilution

Lane 1:

MEF (mouse embryo fibroblast) whole cell lysate at 30 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 30 µg

Lane 3:

B16-F10 (mouse skin melanoma cell) whole cell lysate at 30 µg

Lane 4:

Mouse colon tissue lysate at 30 µg

Lane 5:

Mouse testis tissue lysate at 30 µg

Lane 6:

Mouse heart tissue lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,20 kDa,21 kDa,36 kDa

false

Exposure time: 180s

Carrier free

Anti-FGF2 antibody [EPR29019-77] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29019-77

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.

Biological function summary

Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.

Pathways

FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.

Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Also acts as an integrin ligand which is required for FGF2 signaling (By similarity). Binds to integrin ITGAV : ITGB3 (By similarity). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (By similarity). Functions as a potent mitogen in vitro (By similarity). Can induce angiogenesis (By similarity). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (By similarity).

See full target information Fgf2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 13:1636932 PubMed41040419

2025

Performance study of ZnO-TPU/CS bilayer composite electrospinning scaffold in skin wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlong Wang,Guoxing Huang,Quan Qin,Nianhua Dan,Xinlou Li,Kai Sun,Yuan Yang,Meng Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF2 antibody [EPR29019-77]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Western blot - Anti-FGF2 antibody [EPR29019-77] (AB321990)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise