Anti-FGF21 antibody [EPR8314(2)] ab171941 is a rabbit monoclonal antibody that is used in FGF21 western blotting and IHC. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR8314(2) is the most widely used clone for FGF21 on the market
- Specificity confirmed with FGF21 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Images
![Western blot - Anti-FGF21 antibody [EPR8314(2)] (AB171941), expandable thumbnail](https://content.abcam.com/products/images/fgf21-antibody-epr83142-ab171941--western-blot-img86803.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF21 antibody [EPR8314(2)] (AB171941)")
![Western blot - Anti-FGF21 antibody [EPR8314(2)] (AB171941), expandable thumbnail](https://content.abcam.com/products/images/fgf21-antibody-epr83142-ab171941--western-blot-img162296.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF21 antibody [EPR8314(2)] (AB171941)")
![Western blot - Anti-FGF21 antibody [EPR8314(2)] (AB171941), expandable thumbnail](https://content.abcam.com/products/images/fgf21-antibody-epr83142-ab171941--western-blot-img437869.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF21 antibody [EPR8314(2)] (AB171941)")
Publications
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- Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:1200-12082023Bo's abdominal acupuncture improves disordered metabolism in obese type 2 diabetic rats through regulating fibroblast growth factor 21 and its related adipokines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Xihui,Pang Jianli,Xiong Guan,Feng JiePubMed 37946482
- Research (Washington, D.C.) 6:01752023LonP1 Links Mitochondria-ER Interaction to Regulate Heart Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujie Li,Dawei Huang,Lianqun Jia,Fugen Shangguan,Shiwei Gong,Linhua Lan,Zhiyin Song,Juan Xu,Chaojun Yan,Tongke Chen,Yin Tan,Yongzhang Liu,Xingxu Huang,Carolyn K Suzuki,Zhongzhou Yang,Guanlin Yang,Bin LuPubMed 37333972
- Heliyon 9:e149522023FGF21/FGFR1-β-KL cascade in cardiomyocytes modulates angiogenesis and inflammation under metabolic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamrita Kaur,Sanskruti Ravindra Gare,Andrea Ruiz-Velasco,Jessica M Miller,Riham R E Abouleisa,Qinghui Ou,Jiahan Shen,Handrean Soran,Tamer M A Mohamed,Wei LiuPubMed 37123894
- Physiological reports 11:e156202023Short-term semaglutide treatment improves FGF21 responsiveness in primary hepatocytes isolated from high fat diet challenged mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Nuo Feng,Weijuan Shao,Tianru JinPubMed 36905134
- Frontiers in endocrinology 14:10590202023Fibroblast growth factor 21 is expressed and secreted from skeletal muscle following electrical stimulation extracellular ATP activation of the PI3K/Akt/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuel Arias-Calderón,Mariana Casas,Julián Balanta-Melo,Camilo Morales-Jiménez,Nadia Hernández,Paola Llanos,Enrique Jaimovich,Sonja BuvinicPubMed 36909316
- Life (Basel, Switzerland) 13:2023Localization of FGF21 Protein and Lipid Metabolism-Related Genes in Camels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Gao,Shuqin Zhao,Wangdong Zhang,Huaping Tang,Meilin Yan,Fang Yong,Xu Bai,Xiaochun Wu,Yong Zhang,Quanwei ZhangPubMed 36836789
- Journal of cardiovascular pharmacology 81:150-1642023HDAC Inhibitors Alleviate Uric Acid-Induced Vascular Endothelial Cell Injury by Way of the HDAC6/FGF21/PI3K/AKT Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaihao Wang,Youhong Zhang,Min Zhou,Yipeng Du,Peixin Li,Chang Guan,Zheng HuangPubMed 36607630
- PeerJ 11:e147852023Bidirectional effect of vitamin D on brown adipogenesis of C3H10T1/2 fibroblast-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakako Mukai,Tatsuya KusudoPubMed 36815991
- Cell reports 40:1112392022Central FGF21 production regulates memory but not peripheral metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBolu Zhou,Kristin E Claflin,Kyle H Flippo,Andrew I Sullivan,Arvand Asghari,Satya M Tadinada,Sharon O Jensen-Cody,Ted Abel,Matthew J PotthoffPubMed 36001982
- Animals : an open access journal from MDPI 12:2022Transcriptome Comparison Reveals the Difference in Liver Fat Metabolism between Different Sheep Breeds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaotao Li,Meilin Jin,Xiaojuan Fei,Zehu Yuan,Yuqin Wang,Kai Quan,Tingpu Wang,Junxiang Yang,Maochang He,Caihong WeiPubMed 35804549
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Not recommended |
| Rat | Expected | Tested | Not recommended |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
1 product for Alternative Version
Target data
Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
Alternative names
UNQ3115/PRO10196, FGF21, Fibroblast growth factor 21, FGF-21
Recommended products
Anti-FGF21 antibody [EPR8314(2)] ab171941 is a rabbit monoclonal antibody that is used in FGF21 western blotting and IHC. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR8314(2) is the most widely used clone for FGF21 on the market
- Specificity confirmed with FGF21 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8314(2)
- Purification technique
- Affinity purification Protein A
- Specificity
The immunogen used for this product shares 6 continuous identical amino acids with SIKE1. Cross-reactivity with this protein has not been confirmed experimentally.
Expression levels of the target protein vary with sample type and some optimisation may be required (PMID: 27285327). For western blot using cell lines, it may be necessary to collect cell culture supernatant for endogenous FGF21 detection as the target protein is readily secreted (PMID: 24041694; PMID: 26691139).
ab171941 is not recommended for mouse WB or human IHC-P.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Allow to warm to room temp and agitate gently before aliquoting
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.
- Biological function summary
FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.
- Pathways
FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.
- Associated diseases and disorders
FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941), expandable thumbnail](https://content.abcam.com/products/images/fgf21-antibody-epr83142-ab171941--western-blot-img86803.jpg "Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)")
Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution
All lanes: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
![Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941), expandable thumbnail](https://content.abcam.com/products/images/fgf21-antibody-epr83142-ab171941--western-blot-img162296.jpg "Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)")
Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution
All lanes: Human fetal liver tissue lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
![Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941), expandable thumbnail](https://content.abcam.com/products/images/fgf21-antibody-epr83142-ab171941--western-blot-img437869.jpg "Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)")
Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
False colour image of Western blot: Anti-FGF21 antibody [EPR8314(2)] staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab171941 was shown to bind specifically to FGF21. A band was observed at 21 kDa in treated wild-type HeLa cell lysates with no signal observed at this size in FGF21 knockout cell line Human FGF21 knockout HeLa cell line ab265974 (knockout cell lysate Human FGF21 knockout HeLa cell lysate ab256915). To generate this image, wild-type and FGF21 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development and with high-sensitivity chemiluminescence substrate and imaged with 16 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed at 1/20000 dilution.
All lanes: Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution
Lane 1: Wild-type HeLa Treated BFA (5 ug/mL, 6 h) cell lysate at 20 µg
Lane 2: FGF21 knockout HeLa Treated BFA (5 ug/mL, 6 h) cell lysate at 20 µg
Lane 3: Wild-type HeLa Vehicle Control BFA (0 ug/mL, 6 h) cell lysate at 20 µg
Lane 4: FGF21 knockout HeLa Vehicle Control BFA (0 ug/mL, 6 h) cell lysate at 20 µg
Lane 5: HepG2 Treated BFA (5 ug/mL, 6 h) cell lysate at 20 µg
Lane 6: HepG2 cell lysate at 20 µg
Lane 7: Empty
Lane 8: Human Liver cell lysate at 10 µg
SecondaryAll lanes: HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 21 kDa
Downloads
Product protocols
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- Visit troubleshooting
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