Rabbit Recombinant Monoclonal FGF 23 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--western-blot-img405518.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421)")
![Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--western-blot-img405519.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421)")
![Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--western-blot-img405517.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421)")
![Flow Cytometry (Intracellular) - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--flow-cytometry-intracellular-img405514.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421)")
![Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--western-blot-img405516.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (AB307421)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Tested |
| Mouse | Not recommended | Expected | Expected | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Regulator of phosphate homeostasis (PubMed:11062477). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (PubMed:11409890). Up-regulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (PubMed:15040831). Negatively regulates osteoblast differentiation and matrix mineralization (PubMed:18282132).
Alternative names
HYPF, UNQ3027/PRO9828, FGF23, Fibroblast growth factor 23, FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor
Recommended products
Rabbit Recombinant Monoclonal FGF 23 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25309-57
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fibroblast Growth Factor 23 also known as FGF 23 is a hormone-like protein that plays a role in phosphate and vitamin D metabolism. It is a member of the fibroblast growth factor family and consists of about 251 amino acids with a mass of approximately 32 kDa. FGF 23 is predominantly expressed in bone cells especially osteocytes but it also appears in other tissues such as the thymus and brain. An important tool for quantitative measurement of FGF 23 levels is the FGF 23 ELISA kit which assists in various research and clinical settings.
- Biological function summary
FGF 23 plays an important role in regulating phosphate balance in the body. It impacts the function of kidneys by decreasing the reabsorption of phosphate and suppressing the formation of active vitamin D thereby affecting calcium regulation as well. FGF 23 does not operate alone but interacts with co-receptors such as Klotho to activate its receptor complex. The protein functions as part of a larger system that fine-tunes the balance of minerals important for maintaining bone health and metabolic functions.
- Pathways
FGF 23 is part of the bone-mineral metabolism axis and is integral to the phosphate regulation pathway. It works closely with proteins like Klotho and FGFR1 and their interaction ensures proper signaling required for maintaining serum phosphate levels. FGF 23 controls vitamin D metabolism by regulating the expression of 1-alpha-hydroxylase and 24-hydroxylase influencing the activity of the related vitamin D endocrine system. Its actions help stabilize phosphate and vitamin D status which are both essential for skeletal and mineral homeostasis.
- Associated diseases and disorders
FGF 23 has a significant connection to conditions such as Hypophosphatemic Rickets and Chronic Kidney Disease (CKD). In these cases FGF 23 levels may become dysregulated leading to abnormal phosphate metabolism and bone mineralization defects. In Hypophosphatemic Rickets altered expression of FGF 23 causes excessive phosphate wasting whereas in CKD increased FGF 23 levels are associated with cardiovascular disease risk and deteriorating kidney function. Klotho as a coreceptor for FGF 23 also influences these pathological conditions highlighting its contribution to phosphate-related disorder mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--western-blot-img405518.jpg "Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)")
Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)
This data was developed using Anti-FGF23 antibody [EPR25309-57] ab307420, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
The identity of the bands between 90 kDa and 160 kDa are unknown.
All lanes: Western blot - Anti-FGF23 antibody [EPR25309-57] (Anti-FGF23 antibody [EPR25309-57] ab307420) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HaCaT (human skin keratinocyte) whole cell lysate at 20 µg
Lane 3: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: RAW264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 6: bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 33 kDa
Exposure time: 180s
![Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--western-blot-img405519.jpg "Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)")
Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)
This data was developed using 307420, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: brain (PMID: 20667984).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-FGF23 antibody [EPR25309-57] (Anti-FGF23 antibody [EPR25309-57] ab307420) at 1/1000 dilution
Lane 1: Mouse thymus tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 33 kDa
Exposure time: 180s
![Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--western-blot-img405517.jpg "Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)")
Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)
This data was developed using Anti-FGF23 antibody [EPR25309-57] ab307420, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
The identity of the higher MW band at approximately 140 kDa may represent FGF23 oligermisation. This has not been confirmed with additional tests.
All lanes: Western blot - Anti-FGF23 antibody [EPR25309-57] (Anti-FGF23 antibody [EPR25309-57] ab307420) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeti FGF23 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 33 kDa
Exposure time: 180s
![Flow Cytometry (Intracellular) - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--flow-cytometry-intracellular-img405514.jpg "Flow Cytometry (Intracellular) - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)")
Flow Cytometry (Intracellular) - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)
This data was developed using Anti-FGF23 antibody [EPR25309-57] ab307420, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HaCaT (human skin keratinocyte) cells labeling FGF 23 with Anti-FGF23 antibody [EPR25309-57] ab307420 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--western-blot-img405516.jpg "Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)")
Western blot - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)
This data was developed using 307420, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration:
10 seconds
Exposure time:All lanes: Western blot - Anti-FGF23 antibody [EPR25309-57] (Anti-FGF23 antibody [EPR25309-57] ab307420) at 1/1000 dilution
All lanes: 293T cells transfected with a human FGF23 expression vector containi a myc-his tag whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 33 kDa
Exposure time: 10s
![Immunocytochemistry/ Immunofluorescence - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--immunocytochemistry-immunofluorescence-img405515.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)")
Immunocytochemistry/ Immunofluorescence - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)
This data was developed using Anti-FGF23 antibody [EPR25309-57] ab307420, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeABilized HaCaT (human skin keratinocyte) cells lABelling FGF 23 with Anti-FGF23 antibody [EPR25309-57] ab307420 at 1/50 (9.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in HaCaT cells.The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421), expandable thumbnail](https://content.abcam.com/products/images/fgf23-antibody-epr25309-57-bsa-and-azide-free-ab307421--immunocytochemistry-immunofluorescence-img405515.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FGF23 antibody [EPR25309-57] - BSA and Azide free (ab307421)")
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