JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB65974

Anti-FGF4 antibody

1

(1 Review)

|

(8 Publications)

Rabbit Polyclonal FGF4 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human FGF4 aa 50-100.

View Alternative Names

HST, HSTF1, KS3, FGF4, Fibroblast growth factor 4, FGF-4, Heparin secretory-transforming protein 1, Heparin-binding growth factor 4, Transforming protein KS3, HST-1, HSTF-1, HBGF-4

1 Images
Western blot - Anti-FGF4 antibody (AB65974)
  • WB

Unknown

Western blot - Anti-FGF4 antibody (AB65974)

All lanes:

Western blot - Anti-FGF4 antibody (ab65974) at 1 µg/mL

All lanes:

Hela cell lysis at 30 µg

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human FGF4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P08620

Specificity

We have received data from a collaborator showing that ab65974 does not work on mouse in ICC. We do not recommend using ab65974 for this species/application combination.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 0.2mL
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FGF4 protein also known as fibroblast growth factor 4 is an important player in cell proliferation differentiation and survival. It is part of the fibroblast growth factor family which consists of over 20 known members. The FGF4 protein has a mass of approximately 22 kDa. Researchers observe its expression in various tissues including the embryo and certain adult tissues where it contributes to developmental processes and tissue repair. FGF4 signalling plays a significant role in embryogenesis.
Biological function summary

FGF4 protein regulates pathways involved in cell development and morphogenesis. It particularly influences the formation of limbs and the development of the nervous system. FGF4 usually functions as a part of signaling complexes interacting with its specific receptors on the cell surface. These interactions will lead to a cascade of downstream signaling events that modulate gene expression and cellular behavior.

Pathways

FGF4 engages with multiple signaling pathways notably the MAPK/ERK pathway and the PI3K/AKT pathway. Through these pathways FGF4 protein orchestrates cellular responses important for proper tissue organization and differentiation. It often functions alongside other proteins like FGF2 and FGF8 which share overlapping roles in similar developmental and cellular processes. These interactions underline the complexity and redundancy within the fibroblast growth factor family in ensuring robust biological outcomes.

Dysregulation of FGF4 protein associates with conditions like cancer and developmental defects. Overactive FGF4 signaling has been linked to tumor growth and progression as it can drive unchecked cellular proliferation and survival. Furthermore altered FGF4 expression may relate to syndromes affecting limb development. In the context of these diseases FGF4 can interact with proteins such as FGFR1 leading to aberrant signaling pathways contributing to pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis. May play a role in embryonic molar tooth bud development via inducing the expression of MSX1, MSX2 and MSX1-mediated expression of SDC1 in dental mesenchyme cells (By similarity).
See full target information FGF4
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:215 PubMed36973255

2023

HOXB3 drives WNT-activation associated progression in castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shimiao Zhu,Zhao Yang,Zheng Zhang,Hongli Zhang,Songyang Li,Tao Wu,Xuanrong Chen,Jianing Guo,Aixiang Wang,Hao Tian,Jianpeng Yu,Changwen Zhang,Lei Su,Zhiqun Shang,Changyi Quan,Yuanjie Niu

Cytotechnology 74:469-478 PubMed36110154

2022

MicroRNA-106b-5p (miR-106b-5p) suppresses the proliferation and metastasis of cervical cancer cells via down-regulating fibroblast growth factor 4 (FGF4) expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Hongwei,Li Juan,Xu Xiaoying,Fan Zhijun

The journal of gene medicine 22:e3168 PubMed32023352

2020

miR-511 inhibits proliferation and metastasis of breast cancer cells by targeting FGF4.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Ting Yang,Hongchuan Jiang

Developmental dynamics : an official publication of the American Association of Anatomists 249:441-464 PubMed31762125

2019

Developmental mechanisms driving complex tooth shape in reptiles.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Landova Sulcova,Oldrich Zahradnicek,Jana Dumkova,Hana Dosedelova,Jan Krivanek,Marek Hampl,Michaela Kavkova,Tomas Zikmund,Martina Gregorovicova,David Sedmera,Jozef Kaiser,Abigail S Tucker,Marcela Buchtova

Biology of the cell 106:405-19 PubMed25175801

2014

Cell death, cavitation and spontaneous multi-differentiation of dental pulp stem cells-derived spheroids in vitro: a journey to survival and organogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xiao,Yasuo Kumazawa,Hisashi Okamura

Journal of cellular biochemistry 114:2624-36 PubMed23794488

2013

Characterization of human dental pulp cells-derived spheroids in serum-free medium: stem cells in the core.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xiao,Takeki Tsutsui

Reproduction (Cambridge, England) 145:191-201 PubMed23241344

2013

The expression of fibroblast growth factor receptors during early bovine conceptus development and pharmacological analysis of their actions on trophoblast growth in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Manabu Ozawa,Qi-En Yang,Alan D Ealy

Human molecular genetics 18:2899-911 PubMed19439426

2009

Loss of connexin43-mediated gap junctional coupling in the mesenchyme of limb buds leads to altered expression of morphogens in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Radoslaw Dobrowolski,Gerda Hertig,Hildegard Lechner,Philipp Wörsdörfer,Volker Wulf,Nikolai Dicke,Dawid Eckert,Reinhard Bauer,Hubert Schorle,Klaus Willecke
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB106355

Anti-FGF4 antibody

Western blot - Anti-FGF4 antibody (AB106355)

  • 1
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
Primary Antibodies

AB19857

Anti-Oct4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB19857)

  • 4
  • 45
Primary Antibodies

AB63856

Anti-Osteopontin antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody (AB63856)

  • 0
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com