Rabbit Polyclonal FGF4 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human FGF4 aa 50-100.
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- Cell death & disease 14:2152023HOXB3 drives WNT-activation associated progression in castration-resistant prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShimiao Zhu,Zhao Yang,Zheng Zhang,Hongli Zhang,Songyang Li,Tao Wu,Xuanrong Chen,Jianing Guo,Aixiang Wang,Hao Tian,Jianpeng Yu,Changwen Zhang,Lei Su,Zhiqun Shang,Changyi Quan,Yuanjie NiuPubMed 36973255
- The journal of gene medicine 22:e31682020miR-511 inhibits proliferation and metastasis of breast cancer cells by targeting FGF4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Zhang,Ting Yang,Hongchuan JiangPubMed 32023352
- Developmental dynamics : an official publication of the American Association of Anatomists 249:441-4642019Developmental mechanisms driving complex tooth shape in reptiles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie Landova Sulcova,Oldrich Zahradnicek,Jana Dumkova,Hana Dosedelova,Jan Krivanek,Marek Hampl,Michaela Kavkova,Tomas Zikmund,Martina Gregorovicova,David Sedmera,Jozef Kaiser,Abigail S Tucker,Marcela BuchtovaPubMed 31762125
- Biology of the cell 106:405-192014Cell death, cavitation and spontaneous multi-differentiation of dental pulp stem cells-derived spheroids in vitro: a journey to survival and organogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Xiao,Yasuo Kumazawa,Hisashi OkamuraPubMed 25175801
- Journal of cellular biochemistry 114:2624-362013Characterization of human dental pulp cells-derived spheroids in serum-free medium: stem cells in the core.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Xiao,Takeki TsutsuiPubMed 23794488
- Reproduction (Cambridge, England) 145:191-2012013The expression of fibroblast growth factor receptors during early bovine conceptus development and pharmacological analysis of their actions on trophoblast growth in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManabu Ozawa,Qi-En Yang,Alan D EalyPubMed 23241344
- Human molecular genetics 18:2899-9112009Loss of connexin43-mediated gap junctional coupling in the mesenchyme of limb buds leads to altered expression of morphogens in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRadoslaw Dobrowolski et. al.PubMed 19439426
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FGF4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P08620
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Associated Products
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Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis. May play a role in embryonic molar tooth bud development via inducing the expression of MSX1, MSX2 and MSX1-mediated expression of SDC1 in dental mesenchyme cells (By similarity).
Alternative names
HST, HSTF1, KS3, FGF4, Fibroblast growth factor 4, FGF-4, Heparin secretory-transforming protein 1, Heparin-binding growth factor 4, Transforming protein KS3, HST-1, HSTF-1, HBGF-4
Recommended products
Rabbit Polyclonal FGF4 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human FGF4 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FGF4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P08620
- Purification technique
- Affinity purification Immunogen
- Specificity
We have received data from a collaborator showing that ab65974 does not work on mouse in ICC. We do not recommend using ab65974 for this species/application combination.
- Reconstitution
- reconstitute with water at 0.2mL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FGF4 protein also known as fibroblast growth factor 4 is an important player in cell proliferation differentiation and survival. It is part of the fibroblast growth factor family which consists of over 20 known members. The FGF4 protein has a mass of approximately 22 kDa. Researchers observe its expression in various tissues including the embryo and certain adult tissues where it contributes to developmental processes and tissue repair. FGF4 signalling plays a significant role in embryogenesis.
- Biological function summary
FGF4 protein regulates pathways involved in cell development and morphogenesis. It particularly influences the formation of limbs and the development of the nervous system. FGF4 usually functions as a part of signaling complexes interacting with its specific receptors on the cell surface. These interactions will lead to a cascade of downstream signaling events that modulate gene expression and cellular behavior.
- Pathways
FGF4 engages with multiple signaling pathways notably the MAPK/ERK pathway and the PI3K/AKT pathway. Through these pathways FGF4 protein orchestrates cellular responses important for proper tissue organization and differentiation. It often functions alongside other proteins like FGF2 and FGF8 which share overlapping roles in similar developmental and cellular processes. These interactions underline the complexity and redundancy within the fibroblast growth factor family in ensuring robust biological outcomes.
- Associated diseases and disorders
Dysregulation of FGF4 protein associates with conditions like cancer and developmental defects. Overactive FGF4 signaling has been linked to tumor growth and progression as it can drive unchecked cellular proliferation and survival. Furthermore altered FGF4 expression may relate to syndromes affecting limb development. In the context of these diseases FGF4 can interact with proteins such as FGFR1 leading to aberrant signaling pathways contributing to pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-FGF4 antibody (ab65974)
All lanes: Western blot - Anti-FGF4 antibody (ab65974) at 1 µg/mL
All lanes: Hela cell lysis at 30 µg
Predicted band size: 22 kDa
Observed band size: 22 kDa
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