AB88118

Anti-FGF5 antibody

Name: Anti-FGF5 antibody (ab88118) | Abcam
Brand: Abcam Limited
SKU: AB88118
Price: 430 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(7 Publications)

Rabbit Polyclonal FGF5 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications.

View Alternative Names

Fibroblast growth factor 5, FGF-5, Heparin-binding growth factor 5, Smag-82, HBGF-5, FGF5

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-FGF5 antibody (AB88118)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FGF5 antibody (AB88118)

ab88118 staining FGF5 in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab88118 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Western blot - Anti-FGF5 antibody (AB88118)

All lanes:

Western blot - Anti-FGF5 antibody (ab88118) at 1 µg/mL

Lane 1:

E14 Mouse Embryo Brain Tissue Lysate at 10 µg

Lane 2:

E16 Ms Embryo Brain Tissue Lysate at 10 µg

Lane 3:

E18 Ms Embryo Brain Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 125 kDa,30 kDa

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Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF5 also known as fibroblast growth factor 5 is a protein involved in various cellular processes with a molecular mass of approximately 29 kDa. It is part of the FGF family which includes at least 22 members playing roles in cell growth differentiation and regulation. FGF5 expression occurs mainly in hair follicles skeletal muscles and the central nervous system. The protein has been identified in epithelial cells and the stromal compartment surrounding hair follicles.

Biological function summary

FGF5 functions as a signaling molecule that influences hair growth cycle regulation. It is an important player in the transition of hair follicles from the anagen (growth) phase to the catagen (regression) phase. FGF5 does not act alone but interacts with various cellular receptors particularly FGFR1 and FGFR2 which facilitate its role in hair cycle modulation. It is not typically part of a stable multi-protein complex but works as a soluble factor influencing nearby cell behavior.

Pathways

FGF5 engages with signaling cascades such as the MAPK/ERK pathway and the PI3K/AKT pathway which are significant for cell proliferation and survival. FGF5 can modulate these pathways by binding to FGFR1 and FGFR2 receptors leading to downstream signaling that affects cell behavior. These pathways illustrate its involvement with several other FGF family members such as FGF7 and FGF10 showcasing a broad effect on cellular dynamics and development.

FGF5 has been linked to conditions such as congenital hypotrichosis and alopecia where abnormalities in hair growth cycles occur. Dysfunctions in FGF5 signaling can lead to prolonged anagen phases or premature catagen transitions. Additionally FGF5 has connections to cancer development due to its role in cell proliferation and differentiation; aberrations in FGF5 activity can contribute to tumorigenesis. Although not directly linked proteins like FGFR1 can mediate these effects increasing the complexity of its involvement in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase (By similarity).

See full target information FGF5

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106446 PubMed37091250

2023

Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Ban,Wenqiao Hui,Quanchao Duan,Wenfei Liu,Meng Zhang,Xiaofeng Li,Hanyue Kang,Zhenni Shi,Yan Zhang,Jinbin Hou,Xiuzhen Xu,Shoudong Ye,Baowei Zhang,Xiaobin Xu

Cell death discovery 8:344 PubMed35915078

2022

Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C.

Applications

Unspecified application

Species

Unspecified reactive species

Yujian Dai,Jialei Sun,Na He,Liyou An,Chunhui Hou,Fuliang Du

Oncology reports 48: PubMed35866594

2022

miR‑491‑3p functions as a tumor suppressor in non‑small cell lung cancer by targeting fibroblast growth factor 5.

Applications

Unspecified application

Species

Unspecified reactive species

Gai Zhang,Haijian Zheng,Ling Wang

American journal of translational research 12:8147-8161 PubMed33437388

2020

Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yujian Dai,Jinshan Li,Mingyang Li,Zhihui Liu,Jiao Liu,Liyou An,Fuliang Du

Journal of assisted reproduction and genetics : PubMed33047186

2020

Wnt pathway modulation generates blastomere-derived mouse embryonic stem cells with different pluripotency features.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Vila-Cejudo,Sandra Alonso-Alonso,Anna Pujol,Josep Santaló,Elena Ibáñez

Frontiers in cellular neuroscience 14:237 PubMed32848626

2020

FGF5 Regulates Schwann Cell Migration and Adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Chen,Rong Hu,Qing Min,Yankun Li,David B Parkinson,Xin-Peng Dun

Journal of cell science 128:2983-8 PubMed26116571

2015

Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells.

Applications

IHC

Species

Human

Bi-Sen Ding,Kazunori Gomi,Shahin Rafii,Ronald G Crystal,Matthew S Walters

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FGF5 antibody (AB88118)

Western blot - Anti-FGF5 antibody (AB88118)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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