Rabbit Polyclonal FGF5 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications.
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Publications
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- iScience 26:1064462023Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Ban,Wenqiao Hui,Quanchao Duan,Wenfei Liu,Meng Zhang,Xiaofeng Li,Hanyue Kang,Zhenni Shi,Yan Zhang,Jinbin Hou,Xiuzhen Xu,Shoudong Ye,Baowei Zhang,Xiaobin XuPubMed 37091250
- Cell death discovery 8:3442022Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujian Dai,Jialei Sun,Na He,Liyou An,Chunhui Hou,Fuliang DuPubMed 35915078
- American journal of translational research 12:8147-81612020Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujian Dai et. al.PubMed 33437388
- Journal of assisted reproduction and genetics :2020Wnt pathway modulation generates blastomere-derived mouse embryonic stem cells with different pluripotency features.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Vila-Cejudo et. al.PubMed 33047186
- Frontiers in cellular neuroscience 14:2372020FGF5 Regulates Schwann Cell Migration and Adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Chen et. al.PubMed 32848626
- Journal of cell science 128:2983-82015Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Expected |
| Mouse | Tested | Expected |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase (By similarity).
Alternative names
Fibroblast growth factor 5, FGF-5, Heparin-binding growth factor 5, Smag-82, HBGF-5, FGF5
Recommended products
Rabbit Polyclonal FGF5 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF5 also known as fibroblast growth factor 5 is a protein involved in various cellular processes with a molecular mass of approximately 29 kDa. It is part of the FGF family which includes at least 22 members playing roles in cell growth differentiation and regulation. FGF5 expression occurs mainly in hair follicles skeletal muscles and the central nervous system. The protein has been identified in epithelial cells and the stromal compartment surrounding hair follicles.
- Biological function summary
FGF5 functions as a signaling molecule that influences hair growth cycle regulation. It is an important player in the transition of hair follicles from the anagen (growth) phase to the catagen (regression) phase. FGF5 does not act alone but interacts with various cellular receptors particularly FGFR1 and FGFR2 which facilitate its role in hair cycle modulation. It is not typically part of a stable multi-protein complex but works as a soluble factor influencing nearby cell behavior.
- Pathways
FGF5 engages with signaling cascades such as the MAPK/ERK pathway and the PI3K/AKT pathway which are significant for cell proliferation and survival. FGF5 can modulate these pathways by binding to FGFR1 and FGFR2 receptors leading to downstream signaling that affects cell behavior. These pathways illustrate its involvement with several other FGF family members such as FGF7 and FGF10 showcasing a broad effect on cellular dynamics and development.
- Associated diseases and disorders
FGF5 has been linked to conditions such as congenital hypotrichosis and alopecia where abnormalities in hair growth cycles occur. Dysfunctions in FGF5 signaling can lead to prolonged anagen phases or premature catagen transitions. Additionally FGF5 has connections to cancer development due to its role in cell proliferation and differentiation; aberrations in FGF5 activity can contribute to tumorigenesis. Although not directly linked proteins like FGFR1 can mediate these effects increasing the complexity of its involvement in disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-FGF5 antibody (ab88118)
All lanes: Western blot - Anti-FGF5 antibody (ab88118) at 1 µg/mL
Lane 1: E14 Mouse Embryo Brain Tissue Lysate at 10 µg
Lane 2: E16 Ms Embryo Brain Tissue Lysate at 10 µg
Lane 3: E18 Ms Embryo Brain Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 125 kDa, 30 kDa
Exposure time: 2min
Immunocytochemistry/ Immunofluorescence - Anti-FGF5 antibody (ab88118)
ab88118 staining FGF5 in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab88118 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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