AB81384

Anti-FGF8 antibody

Name: Anti-FGF8 antibody (ab81384) | Abcam
Brand: Abcam Limited
SKU: AB81384
Price: 490 USD
Availability: InStock

(1 Review)

(6 Publications)

Rabbit Polyclonal FGF8 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human FGF8.

View Alternative Names

AIGF, FGF8, Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, Heparin-binding growth factor 8, HBGF-8

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

ab81384 staining FGF8 in Human Ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 37°C; antigen retrieval was by microwave heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 μg/mL) for 2 hours at 37°C. A Biotin-conjugated secondary antibody was used.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

ab81384 at 1μg/ml staining FGF8 in Rat ovary tissue sections by Immunohistochemistry(Formalin/ PFA-fixed paraffin-embedded sections). The tissue underwent heat mediated antigen retrieval. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

Unknown

Western blot - Anti-FGF8 antibody (AB81384)

All lanes:

Western blot - Anti-FGF8 antibody (ab81384)

All lanes:

Rat Ovary Tissue Lysate

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human FGF8. The exact immunogen used to generate this antibody is proprietary information.

P55075

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FGF8 protein also known as fibroblast growth factor 8 plays a mechanical role in cell signaling and development. The FGF8 gene encodes two main isoforms: FGF8a and FGF8b each with different biological activities. FGF8 has a molecular mass of approximately 22-24 kDa. It expresses in developing tissues particularly in the embryo during early stages of vertebrate development. FGF8 interacts with fibroblast growth factor receptors (FGFRs) to initiate a cascade of signaling events leading to cellular responses.

Biological function summary

The FGF8 protein influences the regulation of cell division growth and differentiation particularly in embryonic development. FGF8 an integral part of several signaling complexes activates pathways that guide mesoderm and neural plate induction. Its expression is essential for limb and brain development providing necessary signaling cues required for proper patterning and morphogenetic processes.

Pathways

The FGF8 protein plays a central role in the MAPK/ERK signaling and PI3K/AKT pathways important mechanisms that govern cellular proliferation and survival. These pathways involve several proteins such as FGFR1 and FGFR2 which interact with FGF8 to mediate its effects. Through these pathways FGF8 coordinates with other members of the fibroblast growth factor family to regulate cellular functions in a concerted manner.

The FGF8 protein associates with various congenital malformations specifically conditions like Kallmann syndrome and certain craniofacial dysplasias. Mutations or dysregulation in FGF8 can disrupt normal signaling processes leading to abnormalities in development. The protein closely interacts with other growth factors and receptors such as FGFR1 which when altered might contribute to the pathogenesis of these disorders by affecting the signaling pathways they share with FGF8.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed : 16384934, PubMed : 16597617, PubMed : 8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed : 21576111).

See full target information FGF8

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of oral science 13:6 PubMed33649301

2021

FGF8 induces epithelial-mesenchymal transition and promotes metastasis in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yilong Hao,Yanxuan Xiao,Xiaoyu Liao,Shuya Tang,Xiaoyan Xie,Rui Liu,Qianming Chen

Journal of pediatric urology 16:41.e1-41.e10 PubMed31718875

2019

FGF8, FGF10 and FGF receptor 2 in foreskin of children with hypospadias: an analysis of immunohistochemical expression patterns and gene transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Bernhard Haid,Elisabeth Pechriggl,Felix Nägele,Jozsef Dudas,Gerald Webersinke,Melanie Rammer,Helga Fritsch,Josef Oswald

Oncology reports 38:2205-2210 PubMed28791365

2017

FGF8 promotes cell proliferation and resistance to EGFR inhibitors via upregulation of EGFR in human hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanmin Pei,Xueling Sun,Xiwei Guo,Huashan Yin,Le Wang,Fugu Tian,Hongxi Jing,Xiaobo Liang,Jun Xu,Pengcheng Shi

Cellular and molecular life sciences : CMLS 74:4561-4572 PubMed28726057

2017

Macrophage migration inhibitory factor (MIF) modulates trophic signaling through interaction with serine protease HTRA1.

Applications

ICC

Species

Mouse

Åsa Fex Svenningsen,Svenja Löring,Anna Lahn Sørensen,Ha Uyen Buu Huynh,Simone Hjæresen,Nellie Martin,Jesper Bonnet Moeller,Maria Louise Elkjær,Uffe Holmskov,Zsolt Illes,Malin Andersson,Solveig Beck Nielsen,Eirikur Benedikz

Microscopy research and technique 76:321-30 PubMed23362007

2013

Fibroblast growth factor, estrogen, and prolactin receptor features in different grades of prostatic adenocarcinoma in elderly men.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Cia Hetzl,Fabio Montico,Raisa Misitieri Lorencini,Larissa Kido,Eduardo Cândido,Athanase Billis,Ubirajara Ferreira,Valeria Ha Cagnon

Integrative biology : quantitative biosciences fro 3:1179-87 PubMed22028041

2011

Spatially organized in vitro models instruct asymmetric stem cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chin Toh,Katarina Blagovic,Hanry Yu,Joel Voldman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

Western blot - Anti-FGF8 antibody (AB81384)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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