Rabbit Polyclonal FGF8 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human FGF8.
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Publications
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- International journal of oral science 13:62021FGF8 induces epithelial-mesenchymal transition and promotes metastasis in oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYilong Hao,Yanxuan Xiao,Xiaoyu Liao,Shuya Tang,Xiaoyan Xie,Rui Liu,Qianming ChenPubMed 33649301
- Journal of pediatric urology 16:41.e1-41.e102019FGF8, FGF10 and FGF receptor 2 in foreskin of children with hypospadias: an analysis of immunohistochemical expression patterns and gene transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBernhard Haid,Elisabeth Pechriggl,Felix Nägele,Jozsef Dudas,Gerald Webersinke,Melanie Rammer,Helga Fritsch,Josef OswaldPubMed 31718875
- Oncology reports 38:2205-22102017FGF8 promotes cell proliferation and resistance to EGFR inhibitors via upregulation of EGFR in human hepatocellular carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanmin Pei et. al.PubMed 28791365
- Cellular and molecular life sciences : CMLS 74:4561-45722017Macrophage migration inhibitory factor (MIF) modulates trophic signaling through interaction with serine protease HTRA1.
- Microscopy research and technique 76:321-302013Fibroblast growth factor, estrogen, and prolactin receptor features in different grades of prostatic adenocarcinoma in elderly men.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda Cia Hetzl et. al.PubMed 23362007
- Integrative biology : quantitative biosciences fro 3:1179-872011Spatially organized in vitro models instruct asymmetric stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Chin Toh et. al.PubMed 22028041
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FGF8. The exact immunogen used to generate this antibody is proprietary information. Database link P55075
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed:16384934, PubMed:16597617, PubMed:8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed:21576111).
Alternative names
AIGF, FGF8, Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, Heparin-binding growth factor 8, HBGF-8
Recommended products
Rabbit Polyclonal FGF8 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human FGF8.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FGF8. The exact immunogen used to generate this antibody is proprietary information. Database link P55075
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FGF8 protein also known as fibroblast growth factor 8 plays a mechanical role in cell signaling and development. The FGF8 gene encodes two main isoforms: FGF8a and FGF8b each with different biological activities. FGF8 has a molecular mass of approximately 22-24 kDa. It expresses in developing tissues particularly in the embryo during early stages of vertebrate development. FGF8 interacts with fibroblast growth factor receptors (FGFRs) to initiate a cascade of signaling events leading to cellular responses.
- Biological function summary
The FGF8 protein influences the regulation of cell division growth and differentiation particularly in embryonic development. FGF8 an integral part of several signaling complexes activates pathways that guide mesoderm and neural plate induction. Its expression is essential for limb and brain development providing necessary signaling cues required for proper patterning and morphogenetic processes.
- Pathways
The FGF8 protein plays a central role in the MAPK/ERK signaling and PI3K/AKT pathways important mechanisms that govern cellular proliferation and survival. These pathways involve several proteins such as FGFR1 and FGFR2 which interact with FGF8 to mediate its effects. Through these pathways FGF8 coordinates with other members of the fibroblast growth factor family to regulate cellular functions in a concerted manner.
- Associated diseases and disorders
The FGF8 protein associates with various congenital malformations specifically conditions like Kallmann syndrome and certain craniofacial dysplasias. Mutations or dysregulation in FGF8 can disrupt normal signaling processes leading to abnormalities in development. The protein closely interacts with other growth factors and receptors such as FGFR1 which when altered might contribute to the pathogenesis of these disorders by affecting the signaling pathways they share with FGF8.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (ab81384)
ab81384 staining FGF8 in Human Ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 37°C; antigen retrieval was by microwave heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 μg/mL) for 2 hours at 37°C. A Biotin-conjugated secondary antibody was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (ab81384)
ab81384 at 1μg/ml staining FGF8 in Rat ovary tissue sections by Immunohistochemistry(Formalin/ PFA-fixed paraffin-embedded sections). The tissue underwent heat mediated antigen retrieval. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
Western blot - Anti-FGF8 antibody (ab81384)
All lanes: Western blot - Anti-FGF8 antibody (ab81384)
All lanes: Rat Ovary Tissue Lysate
Predicted band size: 27 kDa
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