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AB89550

Anti-FGF8 antibody [MM0291-8D24]

5

(1 Review)

|

(1 Publication)

Mouse Monoclonal FGF8 antibody. Suitable for WB, IHC-P, FuncS (Neut/Block) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FGF8.

View Alternative Names

AIGF, FGF8, Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, Heparin-binding growth factor 8, HBGF-8

1 Images
Western blot - Anti-FGF8 antibody [MM0291-8D24] (AB89550)
  • WB

Supplier Data

Western blot - Anti-FGF8 antibody [MM0291-8D24] (AB89550)

All lanes:

Western blot - Anti-FGF8 antibody [MM0291-8D24] (ab89550) at 1/500 dilution

All lanes:

Human placenta tissue lysate

Predicted band size: 27 kDa

Observed band size: 23 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0291-8D24

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

FuncS (Neut/Block), WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FGF8.

P55075

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FGF8 protein also known as fibroblast growth factor 8 plays a mechanical role in cell signaling and development. The FGF8 gene encodes two main isoforms: FGF8a and FGF8b each with different biological activities. FGF8 has a molecular mass of approximately 22-24 kDa. It expresses in developing tissues particularly in the embryo during early stages of vertebrate development. FGF8 interacts with fibroblast growth factor receptors (FGFRs) to initiate a cascade of signaling events leading to cellular responses.
Biological function summary

The FGF8 protein influences the regulation of cell division growth and differentiation particularly in embryonic development. FGF8 an integral part of several signaling complexes activates pathways that guide mesoderm and neural plate induction. Its expression is essential for limb and brain development providing necessary signaling cues required for proper patterning and morphogenetic processes.

Pathways

The FGF8 protein plays a central role in the MAPK/ERK signaling and PI3K/AKT pathways important mechanisms that govern cellular proliferation and survival. These pathways involve several proteins such as FGFR1 and FGFR2 which interact with FGF8 to mediate its effects. Through these pathways FGF8 coordinates with other members of the fibroblast growth factor family to regulate cellular functions in a concerted manner.

The FGF8 protein associates with various congenital malformations specifically conditions like Kallmann syndrome and certain craniofacial dysplasias. Mutations or dysregulation in FGF8 can disrupt normal signaling processes leading to abnormalities in development. The protein closely interacts with other growth factors and receptors such as FGFR1 which when altered might contribute to the pathogenesis of these disorders by affecting the signaling pathways they share with FGF8.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed : 16384934, PubMed : 16597617, PubMed : 8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed : 21576111).
See full target information FGF8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of respiratory and critical care medicine 185:660-9 PubMed22199006

2011

Differentiation and recruitment of IL-22-producing helper T cells stimulated by pleural mesothelial cells in tuberculous pleurisy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Jian Ye,Qiong Zhou,Ming-Li Yuan,Rong-Hui Du,Wei-Bing Yang,Xian-Zhi Xiong,Bo Huang,Huan-Zhong Shi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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