Rabbit Recombinant Monoclonal FGF9/GAF antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 8 publications.
Images
![Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408), expandable thumbnail](https://content.abcam.com/products/images/fgf9-gaf-antibody-epr19937-ab206408--western-blot-img64862.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)")
![Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408), expandable thumbnail](https://content.abcam.com/products/images/fgf9-gaf-antibody-epr19937-ab206408--western-blot-img147999.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)")
![Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408), expandable thumbnail](https://content.abcam.com/products/images/fgf9-gaf-antibody-epr19937-ab206408--western-blot-img148119.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)")
![Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408), expandable thumbnail](https://content.abcam.com/products/images/fgf9-gaf-antibody-epr19937-ab206408--western-blot-img145646.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)")
Publications
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- Analytical cellular pathology (Amsterdam) 2023:59115462023Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Sun,Sanmao Su,Mengjun Li,Ang DengPubMed 37035017
- Frontiers in pharmacology 13:8501282022FGF9 Alleviates the Fatty Liver Phenotype by Regulating Hepatic Lipid Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanrong Zhao,Lei Zhang,Menglin Zhang,Jincan Huang,Jun Zhang,Yongsheng ChangPubMed 35517790
- Experimental and therapeutic medicine 23:1312022Knockdown of long non-coding RNA NEAT1 relieves inflammation of ulcerative colitis by regulating the miR-603/FGF9 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengdong Li,Hui Liu,Jinjin Fu,Li Fan,Shuangshuang Lu,Huahui Zhang,Zhanju LiuPubMed 34970354
- American journal of translational research 13:13674-136822021Oxymatrine inhibits proliferation and apoptosis of human breast cancer cells through the regulation of miRNA-140-5P.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Xiao,Xiaolei Hou,Wenda Shi,Chanchan Hu,Yanan Cui,Jiuli Hu,Zongfang Piao,Xiaoqin Zhu,Qingshan Li,Fan XuPubMed 35035706
- Molecular medicine reports 24:2021miR‑486‑5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiwei Wei,Chunyu Liu,Rongrong Yao,Qingyun Tan,Qingdong Wang,Hao TianPubMed 34490480
- Stem cell research & therapy 12:3442021Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuemei Fan,Sheng He,Huifang Song,Wenjuan Yin,Jie Zhang,Zexu Peng,Kun Yang,Xiaoyan Zhai,Lingxia Zhao,Hui Gong,Yi Ping,Xiangying Jiao,Sanyuan Zhang,Changping Yan,Hongliang Wang,Ren-Ke Li,Jun XiePubMed 34112245
- Medicine 100:e234232021LncRNA NEAT1 promotes proliferation of ovarian cancer cells and angiogenesis of co-incubated human umbilical vein endothelial cells by regulating FGF9 through sponging miR-365: An experimental study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialing Yuan,Ke Yi,Lingyun YangPubMed 33545926
- Scientific reports 9:196012019Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasahiro Asakawa et. al.PubMed 31862949
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Recombinant full length protein - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Alternative names
Fibroblast growth factor 9, FGF-9, Glia-activating factor, Heparin-binding growth factor 9, GAF, HBGF-9, FGF9
Recommended products
Rabbit Recombinant Monoclonal FGF9/GAF antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19937
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF9 also known as GAF is a member of the fibroblast growth factor family. The FGF9 protein has a molecular mass of approximately 23 kDa. It is commonly expressed in the brain kidney and lungs during fetal development. The FGF9 protein functions as a signaling molecule influencing cell proliferation and differentiation which are essential for proper tissue formation and organogenesis.
- Biological function summary
FGF9 contributes to important processes in the development and maintenance of several tissues. It is not known to be part of a larger complex. FGF9 regulates cellular activities such as proliferation and differentiation through its interaction with specific receptors on the cell surface. Its role is significant in skeletal and nervous system development where it ensures proper maturation and function of these systems.
- Pathways
The FGF9 protein participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell survival proliferation and differentiation. FGF9 interacts closely with other fibroblast growth factors such as FGF2 to ensure effective signaling cascades. These interactions affect various cellular responses and contribute to the normal development of multiple organ systems.
- Associated diseases and disorders
Alterations in FGF9 expression can be linked to conditions such as lung fibrosis and cancer. Aberrant FGF9 signaling can contribute to uncontrolled cell proliferation and fibrosis formation. The GAF protein's dysregulation may involve interactions with receptor proteins including FGFR2 in these pathological processes. Understanding FGF9's role in these diseases helps in developing targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408), expandable thumbnail](https://content.abcam.com/products/images/fgf9-gaf-antibody-epr19937-ab206408--western-blot-img64862.jpg "Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)")
Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 1 seconds; Lane 3: 15 seconds.
Human FGF9/GAF active protein contains aa1-208; FGF16 and FGF20 recombinant protein contain aa1-203 and aa4-208 respectively, both with His-Tag®.
All lanes: Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1: Human FGF9/GAF active protein at 0.01 µg
Lane 2: Human FGF16 recombinant protein at 0.01 µg
Lane 3: Human FGF20 recombinant protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
![Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408), expandable thumbnail](https://content.abcam.com/products/images/fgf9-gaf-antibody-epr19937-ab206408--western-blot-img147999.jpg "Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)")
Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 15 seconds; Lane 3: 30 seconds.
The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).
All lanes: Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human fetal kidney lysate at 10 µg
Lane 3: Human ovary cancer lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa
![Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408), expandable thumbnail](https://content.abcam.com/products/images/fgf9-gaf-antibody-epr19937-ab206408--western-blot-img148119.jpg "Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)")
Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 15 seconds; Lane 3: 30 seconds.
The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).
All lanes: Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1: Mouse kidney lysate at 10 µg
Lane 2: Mouse brain lysate at 10 µg
Lane 3: Rat brain lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa
![Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408), expandable thumbnail](https://content.abcam.com/products/images/fgf9-gaf-antibody-epr19937-ab206408--western-blot-img145646.jpg "Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)")
Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-3: 15 seconds; Lane 4: 3 minutes.
The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).
All lanes: Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 2: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 4: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa
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