AB206408

Anti-FGF9/GAF antibody [EPR19937]

RabMAb
Recombinant
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(11 Publications)

Rabbit Recombinant Monoclonal FGF9/GAF antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 11 publications.

View Alternative Names

Fibroblast growth factor 9, FGF-9, Glia-activating factor, Heparin-binding growth factor 9, GAF, HBGF-9, FGF9

4 Images

Supplier Data

Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1-3 : 15 seconds; Lane 4 : 3 minutes.

The MW observed is consistent with the literature (PMID : 16452189, PMID : 25925261).

All lanes:

Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

false

Supplier Data

Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1/2 : 15 seconds; Lane 3 : 30 seconds.

The MW observed is consistent with the literature (PMID : 16452189, PMID : 25925261).

All lanes:

Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human ovary cancer lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

false

Unknown

Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1/2 : 1 seconds; Lane 3 : 15 seconds.

Human FGF9/GAF active protein contains aa1-208; FGF16 and FGF20 recombinant protein contain aa1-203 and aa4-208 respectively, both with His-Tag®.

All lanes:

Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

Lane 1:

Human FGF9/GAF active protein at 0.01 µg

Lane 2:

Human FGF16 recombinant protein at 0.01 µg

Lane 3:

Human FGF20 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Supplier Data

Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1/2 : 15 seconds; Lane 3 : 30 seconds.

The MW observed is consistent with the literature (PMID : 16452189, PMID : 25925261).

All lanes:

Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

false

Carrier free

Anti-FGF9/GAF antibody [EPR19937] - BSA and Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19937

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF9 also known as GAF is a member of the fibroblast growth factor family. The FGF9 protein has a molecular mass of approximately 23 kDa. It is commonly expressed in the brain kidney and lungs during fetal development. The FGF9 protein functions as a signaling molecule influencing cell proliferation and differentiation which are essential for proper tissue formation and organogenesis.

Biological function summary

FGF9 contributes to important processes in the development and maintenance of several tissues. It is not known to be part of a larger complex. FGF9 regulates cellular activities such as proliferation and differentiation through its interaction with specific receptors on the cell surface. Its role is significant in skeletal and nervous system development where it ensures proper maturation and function of these systems.

Pathways

The FGF9 protein participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell survival proliferation and differentiation. FGF9 interacts closely with other fibroblast growth factors such as FGF2 to ensure effective signaling cascades. These interactions affect various cellular responses and contribute to the normal development of multiple organ systems.

Alterations in FGF9 expression can be linked to conditions such as lung fibrosis and cancer. Aberrant FGF9 signaling can contribute to uncontrolled cell proliferation and fibrosis formation. The GAF protein's dysregulation may involve interactions with receptor proteins including FGFR2 in these pathological processes. Understanding FGF9's role in these diseases helps in developing targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

See full target information Fibroblast growth factor 9

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

NPJ biofilms and microbiomes 10:142 PubMed39627243

2024

Gingival spatial analysis reveals geographic immunological variation in a microbiota-dependent and -independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Jaber,Avital Sarusi-Portuguez,Yasmin Netanely,Reem Naamneh,Shahd Yacoub,Or Saar,Nadeem Darawshi,Luba Eli-Berchoer,Hagit Shapiro,Eran Elinav,Asaf Wilensky,Avi-Hai Hovav

Journal of cardiothoracic surgery 19:314 PubMed38824534

2024

Long non-coding RNA MEG3 knockdown represses airway smooth muscle cells proliferation and migration via sponging miR-143-3p/FGF9 in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Gu,Dengfeng Zhou

Analytical cellular pathology (Amsterdam) 2023:5911546 PubMed37035017

2023

Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Sun,Sanmao Su,Mengjun Li,Ang Deng

Biomolecules 12: PubMed36358989

2022

FGF9 Promotes Expression of HAS2 in Palatal Elevation via the Wnt/β-Catenin/TCF7L2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yidan Sun,Xiyu Ying,Ruomei Li,Mengjia Weng,Jiajun Shi,Zhenqi Chen

Frontiers in pharmacology 13:850128 PubMed35517790

2022

FGF9 Alleviates the Fatty Liver Phenotype by Regulating Hepatic Lipid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Fanrong Zhao,Lei Zhang,Menglin Zhang,Jincan Huang,Jun Zhang,Yongsheng Chang

Experimental and therapeutic medicine 23:131 PubMed34970354

2022

Knockdown of long non-coding RNA NEAT1 relieves inflammation of ulcerative colitis by regulating the miR-603/FGF9 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fengdong Li,Hui Liu,Jinjin Fu,Li Fan,Shuangshuang Lu,Huahui Zhang,Zhanju Liu

American journal of translational research 13:13674-13682 PubMed35035706

2021

Oxymatrine inhibits proliferation and apoptosis of human breast cancer cells through the regulation of miRNA-140-5P.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Xiao,Xiaolei Hou,Wenda Shi,Chanchan Hu,Yanan Cui,Jiuli Hu,Zongfang Piao,Xiaoqin Zhu,Qingshan Li,Fan Xu

Molecular medicine reports 24: PubMed34490480

2021

miR‑486‑5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Wei,Chunyu Liu,Rongrong Yao,Qingyun Tan,Qingdong Wang,Hao Tian

Stem cell research & therapy 12:344 PubMed34112245

2021

Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Fan,Sheng He,Huifang Song,Wenjuan Yin,Jie Zhang,Zexu Peng,Kun Yang,Xiaoyan Zhai,Lingxia Zhao,Hui Gong,Yi Ping,Xiangying Jiao,Sanyuan Zhang,Changping Yan,Hongliang Wang,Ren-Ke Li,Jun Xie

Medicine 100:e23423 PubMed33545926

2021

LncRNA NEAT1 promotes proliferation of ovarian cancer cells and angiogenesis of co-incubated human umbilical vein endothelial cells by regulating FGF9 through sponging miR-365: An experimental study.

Applications

Unspecified application

Species

Unspecified reactive species

Jialing Yuan,Ke Yi,Lingyun Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF9/GAF antibody [EPR19937]

Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)

Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)

Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)

Western blot - Anti-FGF9/GAF antibody [EPR19937] (AB206408)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Proteins & Peptides

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise