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Rabbit Monoclonal FGFR1 phospho Y654 antibody. Suitable for WB, I-ELISA, Dot and reacts with Recombinant fragment - Human, Synthetic peptide - Human, Synthetic peptide samples.

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Images

Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043), expandable thumbnail
  • OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043), expandable thumbnail
  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043), expandable thumbnail
  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043), expandable thumbnail
  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIPIHC-PICC/IFWBI-ELISADot
Human
Not recommended
Not recommended
Not recommended
Not recommended
Predicted
Predicted
Predicted
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Predicted
Predicted
Predicted
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Synthetic peptide - Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human, Synthetic peptide - Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human, Synthetic peptide - Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human, Synthetic peptide - Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human, Synthetic peptide - Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Predicted
Predicted

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
-
Notes

1000 ng/ml

Predicted
Predicted

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

Alternative names

CD331, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, FGFR1, Fibroblast growth factor receptor 1, FGFR-1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, bFGF-R-1, FLT-2

Recommended products

Rabbit Monoclonal FGFR1 phospho Y654 antibody. Suitable for WB, I-ELISA, Dot and reacts with Recombinant fragment - Human, Synthetic peptide - Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP843(2)
Purity
Tissue culture supernatant
Specificity

This antibody recognizes FGFR1 (phospho Y654), FGFR2 (phospho Y657), FGFR3 (phospho Y648) and FGFR4 (phospho Y643).

Dissociation constant
1.6 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FGFR1 alpha also known as Fibroblast Growth Factor Receptor 1 is a transmembrane protein with a mass of approximately 104 kDa. It functions as a receptor tyrosine kinase that mediates phosphorylation events when it binds to its ligand the fibroblast growth factors. Upon activation FGFR1 alpha undergoes dimerization and autophosphorylation on specific tyrosine residues. FGFR1 alpha is widely expressed in tissues like the brain lungs and kidneys indicating its involvement in diverse physiological processes.

Biological function summary

The FGFR1 alpha acts as a significant regulator of cell differentiation proliferation and survival. It forms part of a complex network that is critical in tissue development and homeostasis. The interactions of FGFR1 alpha with its ligands can activate several downstream signaling pathways that influence cell behavior. Its role in neural tissue development is particularly important as it supports early neuronal differentiation and survival.

Pathways

FGFR1 alpha features prominently in the MAPK/ERK and PI3K/AKT signaling cascades. These pathways manage essential cellular functions such as growth metabolism and apoptosis. FGFR1 alpha works closely with other receptors and proteins like SHC and GRB2 which propagate signals through these pathways to achieve precise cellular outcomes. These interactions exemplify the receptor’s embeddedness in tightly regulated signaling networks vital for maintaining cellular functions.

Associated diseases and disorders

FGFR1 alpha mutations and aberrant signaling correlate with conditions such as cancer and skeletal developmental disorders. In particular it has connections to syndromes like Pfeiffer syndrome where FGFR1 mutations lead to cranial deformities. In cancer FGFR1 alpha may partner with oncogenic proteins like FRS2 facilitating a microenvironment conducive to tumor proliferation and angiogenesis. Understanding these relationships assists in identifying therapeutic targets and developing interventions for FGFR1 alpha-related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043), expandable thumbnail

    Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

    All lanes: Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043) at 1/1000 dilution

    Lane 1: Control at 10 µg

    Lane 2: Constitutively active human FGFR1 alpha at 10 µg

    Lane 3: Kinase dead lysates from Xenopus oocytes at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 130 kDa

  • OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043), expandable thumbnail

    OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043), expandable thumbnail

    Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

    Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Substrate solution: p-nitrophenyl phosphate(PNPP).

  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043), expandable thumbnail

    Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

    Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Substrate solution: p-nitrophenyl phosphate(PNPP).

  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043), expandable thumbnail

    Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

    Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Substrate solution: p-nitrophenyl phosphate(PNPP).

  • Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043), expandable thumbnail

    Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

    Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Substrate solution: p-nitrophenyl phosphate(PNPP).

  • Dot Blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043), expandable thumbnail

    Dot Blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)

    Dot blot analysis of ab134043 at 1/1000 dilution. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution.

    Lane 1: FGFR1 non-phospho peptide
    Lane 2: FGFR1 Y653 phospho peptide
    Lane 3: FGFR1 Y654 phospho peptide
    Lane 4: FGFR1 Y653 + Y654 phospho peptide
    Lane 5: FGFR2 non-phospho peptide
    Lane 6: FGFR2 Y656 phospho peptide
    Lane 7: FGFR2 Y657 phospho peptide
    Lane 8: FGFR2 Y656 + Y657 phospho peptide
    Lane 9: FGFR3 non-phospho peptide
    Lane 10: FGFR3 Y647 phospho peptide
    Lane 11: FGFR3 Y648 phospho peptide
    Lane 12: FGFR3 Y647 + Y648 phospho peptide
    Lane 13: FGFR4 non-phospho peptide
    Lane 14: FGFR4 Y642 phospho peptide
    Lane 15: FGFR4 Y643 phospho peptide
    Lane 16: FGFR4 Y642 + Y643 phospho peptide

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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