AB134043

Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]

RabMAb
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Rabbit Monoclonal FGFR1 phospho Y654 antibody. Suitable for WB, I-ELISA, Dot and reacts with Recombinant fragment - Human, Synthetic peptide - Human, Synthetic peptide samples.

View Alternative Names

CD331, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, FGFR1, Fibroblast growth factor receptor 1, FGFR-1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, bFGF-R-1, FLT-2

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Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

I-ELISA

Supplier Data

Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Substrate solution : p-nitrophenyl phosphate(PNPP).

I-ELISA

Supplier Data

Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Substrate solution : p-nitrophenyl phosphate(PNPP).

I-ELISA

Supplier Data

Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Substrate solution : p-nitrophenyl phosphate(PNPP).

I-ELISA

Supplier Data

Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Indirect ELISA analysis of ab134043 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Substrate solution : p-nitrophenyl phosphate(PNPP).

Unknown

Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

All lanes:

Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043) at 1/1000 dilution

Lane 1:

Control at 10 µg

Lane 2:

Constitutively active human FGFR1 alpha at 10 µg

Lane 3:

Kinase dead lysates from Xenopus oocytes at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 130 kDa

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OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Supplier Data

Dot Blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Dot blot analysis of ab134043 at 1/1000 dilution. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution.

Lane 1 : FGFR1 non-phospho peptide
Lane 2 : FGFR1 Y653 phospho peptide
Lane 3 : FGFR1 Y654 phospho peptide
Lane 4 : FGFR1 Y653 + Y654 phospho peptide
Lane 5 : FGFR2 non-phospho peptide
Lane 6 : FGFR2 Y656 phospho peptide
Lane 7 : FGFR2 Y657 phospho peptide
Lane 8 : FGFR2 Y656 + Y657 phospho peptide
Lane 9 : FGFR3 non-phospho peptide
Lane 10 : FGFR3 Y647 phospho peptide
Lane 11 : FGFR3 Y648 phospho peptide
Lane 12 : FGFR3 Y647 + Y648 phospho peptide
Lane 13 : FGFR4 non-phospho peptide
Lane 14 : FGFR4 Y642 phospho peptide
Lane 15 : FGFR4 Y643 phospho peptide
Lane 16 : FGFR4 Y642 + Y643 phospho peptide

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP843(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes FGFR1 (phospho Y654), FGFR2 (phospho Y657), FGFR3 (phospho Y648) and FGFR4 (phospho Y643).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "" }, "Synthetic peptide - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "1000 ng/ml", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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Product details

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFR1 alpha also known as Fibroblast Growth Factor Receptor 1 is a transmembrane protein with a mass of approximately 104 kDa. It functions as a receptor tyrosine kinase that mediates phosphorylation events when it binds to its ligand the fibroblast growth factors. Upon activation FGFR1 alpha undergoes dimerization and autophosphorylation on specific tyrosine residues. FGFR1 alpha is widely expressed in tissues like the brain lungs and kidneys indicating its involvement in diverse physiological processes.

Biological function summary

The FGFR1 alpha acts as a significant regulator of cell differentiation proliferation and survival. It forms part of a complex network that is critical in tissue development and homeostasis. The interactions of FGFR1 alpha with its ligands can activate several downstream signaling pathways that influence cell behavior. Its role in neural tissue development is particularly important as it supports early neuronal differentiation and survival.

Pathways

FGFR1 alpha features prominently in the MAPK/ERK and PI3K/AKT signaling cascades. These pathways manage essential cellular functions such as growth metabolism and apoptosis. FGFR1 alpha works closely with other receptors and proteins like SHC and GRB2 which propagate signals through these pathways to achieve precise cellular outcomes. These interactions exemplify the receptor’s embeddedness in tightly regulated signaling networks vital for maintaining cellular functions.

FGFR1 alpha mutations and aberrant signaling correlate with conditions such as cancer and skeletal developmental disorders. In particular it has connections to syndromes like Pfeiffer syndrome where FGFR1 mutations lead to cranial deformities. In cancer FGFR1 alpha may partner with oncogenic proteins like FRS2 facilitating a microenvironment conducive to tumor proliferation and angiogenesis. Understanding these relationships assists in identifying therapeutic targets and developing interventions for FGFR1 alpha-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

See full target information FGFR1 phospho Y654

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]

Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Indirect ELISA - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Western blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

OI-RD Scanning - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Dot Blot - Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (AB134043)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise