AB10646

Anti-FGFR1 antibody

Name: Anti-FGFR1 antibody (ab10646) | Abcam
Brand: Abcam Limited
SKU: AB10646
Price: 630 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(79 Publications)

Anti-FGFR1 antibody (ab10646) is a rabbit polyclonal antibody detecting FGFR1 in IHC-P, ICC/IF. Suitable for Human.

- Over 60 publications
- Trusted since 2004

View Alternative Names

CD331, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, FGFR1, Fibroblast growth factor receptor 1, FGFR-1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, bFGF-R-1, FLT-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody (AB10646)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody (AB10646)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human umbilical cord sections, labeling FGFR1 with ab10646 at a 5 μg/mL concentration. The secondary used was a biotin-anti-Rabbit IgG Peroxidase.

Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody (AB10646)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody (AB10646)

Immunocytochemical immunofluorescence analysis of methanol fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labelling FGFR1 with ab10646 at a 1/100 dilution. Cells were fixed, then permeabilized. The secondary antibody used was a Goat Anti-Rabbit IgG, Cy3™ conjugate (red). Cells were counterstained with DAPI (blue) to stain nuclei.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody (AB10646)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human umbilical cord sections, labeling FGFR1 with ab10646 at a 1/200 dilution.

Biotinylated secondary followed by avidin-HRP and AEC substrate, hematoxylin counterstain.

Antigen retrieval : 0.1% trypsin for 15 minutes at 37°C.

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody (AB10646)

Immunofluorescence analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells, staining FGFR1 using ab10646.

Top row : Cells were either untreated (left) or treated with FGF2 (50 ng/ml) (right) for 60 minutes.
Bottom row : Cells were permeabilized with digitonin, and either untreated (left) or treated with FGF2 (50 ng/ml) (right) for 60 minutes.

Image from Wang YN et al., J Biol Chem. 2012 May 11;287(20):16869-79. Epub 2012 Mar 28. Fig 1.; doi: 10.1074/jbc.M111.314799; May 11, 2012 The Journal of Biological Chemistry, 287, 16869-16879.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human FGFR1 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P11362

Specificity

ab10646 does not react with human FGFR2 and FGFR3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "The epitopes recognized by the antibody are resistant to routine formalin-fixation and paraffin embedding, and to other fixatives e.g. Methacarn, Bouins solution, ethanol and B5." } } }

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Product details

What is this antibody validated in?
Anti-FGFR1 antibody (ab10646) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-FGFR1 (ab10646) was first used in a scientific publication in 2004 and has been cited over 60 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The product is an affinity-purified antibody prepared from pooled sera.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFR1 also known as fibroblast growth factor receptor 1 is a protein with a molecular weight of approximately 92 kDa. It is a receptor tyrosine kinase that binds to fibroblast growth factors (FGFs) triggering a cascade of downstream signaling pathways. FGFR1 is widely expressed in various tissues including the brain skeletal muscle and the cardiovascular system. The FGFR1 protein plays an essential role in cellular processes such as proliferation differentiation and survival.

Biological function summary

FGFR1 is significant in embryonic development and tissue repair. It does not function alone; rather it forms a complex with fibroblast growth factors and heparan sulfate proteoglycans facilitating receptor dimerization and autophosphorylation. FGFR1 is involved in bone growth angiogenesis and wound healing processes. The interaction of FGFR1 with these complex factors ensures the precise regulation of these critical physiological events in the body.

Pathways

FGFR1 participates actively in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are vital for transmitting signals from the cell surface to the DNA in the cell nucleus regulating gene expression and affecting cell cycle progression. FGFR1 works alongside similar proteins like FGFR2 influencing cell fate decisions. By modulating these pathways FGFR1 ensures a proper response to environmental signals which maintains homeostasis and adapts to physiological needs.

FGFR1 is implicated in conditions such as cancer and skeletal dysplasias. Abnormal FGFR1 signaling due to mutations or overexpression can lead to tumorigenesis contributing to the development of cancers like breast and lung cancer. The protein FGFR2 is often evaluated in parallel for similar oncogenic activities. Moreover FGFR1-related dysregulation in bone development can result in skeletal disorders highlighting its importance in both normal physiology and pathological conditions. Anti-FGFR therapies and FGFR1 ELISA tools are being explored to better understand and tackle these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

See full target information FGFR1

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Publications (79)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 44:489-504 PubMed39340759

2024

Inflammatory Mediators Suppress FGFR2 Expression in Human Keratinocytes to Promote Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Ferrarese,Michael Koch,Artemis Baumann,Liliana Bento-Lopes,Daria Wüst,Ivan Berest,Manfred Kopf,Sabine Werner

European journal of medical research 29:67 PubMed38245787

2024

circNINL facilitates aerobic glycolysis, proliferation, invasion, and migration in lung cancer by sponging miR-3918 to mediate FGFR1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Li,Chun Qiu,DaTong Sun,ShengHui Yang,Lin Wang

Acta biochimica et biophysica Sinica 55:1819-1832 PubMed37867436

2023

NF-κB downstream miR-1262 disturbs colon cancer cell malignant behaviors by targeting FGFR1.

Applications

Unspecified application

Species

Unspecified reactive species

Weilin Zhang,Zhongcheng Huang,Zhigang Xiao,Hui Wang,Qianchao Liao,Zhengru Deng,Deqing Wu,Junjiang Wang,Yong Li

Life (Basel, Switzerland) 13: PubMed36836789

2023

Localization of FGF21 Protein and Lipid Metabolism-Related Genes in Camels.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gao,Shuqin Zhao,Wangdong Zhang,Huaping Tang,Meilin Yan,Fang Yong,Xu Bai,Xiaochun Wu,Yong Zhang,Quanwei Zhang

Oncogene 42:491-500 PubMed36357571

2022

Nuclear FGFR1 promotes pancreatic stellate cell-driven invasion through up-regulation of Neuregulin 1.

Applications

Unspecified application

Species

Unspecified reactive species

Abigail S Coetzee,Edward P Carter,Lucía Rodríguez-Fernández,James Heward,Qiaoying Wang,Saadia A Karim,Lina Boughetane,Christopher Milton,Firat Uyulur,Jennifer P Morton,Hemant M Kocher,Richard P Grose

Applied immunohistochemistry & molecular morphology : AIMM 30:600-608 PubMed36083147

2022

FGFR1 Antibody Validation and Characterization of FGFR1 Protein Expression in ER+ Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Paula I Gonzalez-Ericsson,Alberto Servetto,Luigi Formisano,Violeta Sánchez,Ingrid A Mayer,Carlos L Arteaga,Melinda E Sanders

Nature metabolism 4:901-917 PubMed35879461

2022

Prolonged breastfeeding protects from obesity by hypothalamic action of hepatic FGF21.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Pena-Leon,Cintia Folgueira,Silvia Barja-Fernández,Raquel Pérez-Lois,Natália Da Silva Lima,Marion Martin,Violeta Heras,Sara Martinez-Martinez,Paola Valero,Cristina Iglesias,Mannon Duquenne,Omar Al-Massadi,Daniel Beiroa,Yara Souto,Miguel Fidalgo,Rasika Sowmyalakshmi,Diana Guallar,Juan Cunarro,Cecilia Castelao,Ana Senra,Patricia González-Saenz,Rocío Vázquez-Cobela,Rosaura Leis,Guadalupe Sabio,Helge Mueller-Fielitz,Markus Schwaninger,Miguel López,Sulay Tovar,Felipe F Casanueva,Emmanuel Valjent,Carlos Diéguez,Vincent Prevot,Rubén Nogueiras,Luisa M Seoane

Physiological reports 10:e15247 PubMed35385223

2022

Fibroblast growth factor-23 and parathyroid hormone suppress small intestinal magnesium absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Nasisorn Suksridechacin,Narongrit Thongon

Hepatology communications 6:1574-1588 PubMed35271760

2022

FGF1 Signaling Modulates Biliary Injury and Liver Fibrosis in the Mdr2 Mouse Model of Primary Sclerosing Cholangitis.

Applications

Unspecified application

Species

Unspecified reactive species

April O'Brien,Tianhao Zhou,Tori White,Abigail Medford,Lixian Chen,Konstantina Kyritsi,Nan Wu,Jonathan Childs,Danaleigh Stiles,Ludovica Ceci,Sanjukta Chakraborty,Burcin Ekser,Leonardo Baiocchi,Guido Carpino,Eugenio Gaudio,Chaodong Wu,Lindsey Kennedy,Heather Francis,Gianfranco Alpini,Shannon Glaser

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34921114

2021

N6-methyladenosine (mA) depletion regulates pluripotency exit by activating signaling pathways in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Xuan Jin,Rujuan Zuo,Konstantinos Anastassiadis,Arne Klungland,Carsten Marr,Adam Filipczyk

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGFR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody (AB10646)

Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody (AB10646)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody (AB10646)

Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody (AB10646)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (79)

Product promise