Anti-FGFR1 antibody (ab10646) is a rabbit polyclonal antibody that is used to detect FGFR1 in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Over 60 publications
- Trusted since 2004
Images
Publications
Filter by
- Life (Basel, Switzerland) 13:2023Localization of FGF21 Protein and Lipid Metabolism-Related Genes in Camels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Gao,Shuqin Zhao,Wangdong Zhang,Huaping Tang,Meilin Yan,Fang Yong,Xu Bai,Xiaochun Wu,Yong Zhang,Quanwei ZhangPubMed 36836789
- Oncogene 42:491-5002022Nuclear FGFR1 promotes pancreatic stellate cell-driven invasion through up-regulation of Neuregulin 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbigail S Coetzee,Edward P Carter,Lucía Rodríguez-Fernández,James Heward,Qiaoying Wang,Saadia A Karim,Lina Boughetane,Christopher Milton,Firat Uyulur,Jennifer P Morton,Hemant M Kocher,Richard P GrosePubMed 36357571
- Applied immunohistochemistry & molecular morphology : AIMM 30:600-6082022FGFR1 Antibody Validation and Characterization of FGFR1 Protein Expression in ER+ Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaula I Gonzalez-Ericsson,Alberto Servetto,Luigi Formisano,Violeta Sánchez,Ingrid A Mayer,Carlos L Arteaga,Melinda E SandersPubMed 36083147
- Molecular medicine reports 25:2021Long non‑coding RNA HCG18 facilitates the progression of laryngeal and hypopharyngeal squamous cell carcinoma by upregulating FGFR1 via miR‑133b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongbin Peng,Pingjiang GePubMed 34878161
- Molecular medicine reports 25:2021Knockdown of lncRNA NEAT1 suppresses proliferation and migration, and induces apoptosis of cervical cancer cells by regulating the miR‑377/FGFR1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Geng,Wen-Cong Jia,Tao Li,Na Li,Wei WeiPubMed 34779493
- Clinical cancer research : an official journal of the American Association for Cancer Research 27:4379-43962021Nuclear FGFR1 Regulates Gene Transcription and Promotes Antiestrogen Resistance in ER Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Servetto,Rahul Kollipara,Luigi Formisano,Chang-Ching Lin,Kyung-Min Lee,Dhivya R Sudhan,Paula I Gonzalez-Ericsson,Sumanta Chatterjee,Angel Guerrero-Zotano,Saurabh Mendiratta,Hiroaki Akamatsu,Nicholas James,Roberto Bianco,Ariella B Hanker,Ralf Kittler,Carlos L ArteagaPubMed 34011560
- Diagnostics (Basel, Switzerland) 11:2021High Expression of PD-L1 Is Associated with Better Survival in Pancreatic/Periampullary Cancers and Correlates with Epithelial to Mesenchymal Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNishant Thakur,Kwang Yeol Paik,Gyoyeon Hwang,Yosep ChongPubMed 33810560
- BMC cancer 21:1702021Following cytotoxic nanoconjugates from injection to halting the cell cycle machinery and its therapeutic implications in oral cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHend M Abdel Hamid,Zeinab E Darwish,Sahar M Elsheikh,Ghada M Mourad,Hanaa M Donia,Marwa M AfifiPubMed 33596850
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias Wijshake,Zhongju Zou,Beibei Chen,Lin Zhong,Guanghua Xiao,Yang Xie,John G Doench,Lynda Bennett,Beth LevinePubMed 33495338
- International journal of molecular sciences 22:2020Global Genome Conformational Programming during Neuronal Development Is Associated with CTCF and Nuclear FGFR1-The Genome Archipelago Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrandon Decker et. al.PubMed 33396256
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FGFR1 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P11362
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 | Notes This was determined by blotting using an extract of FGFR-1 transfected cells. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes The epitopes recognized by the antibody are resistant to routine formalin-fixation and paraffin embedding, and to other fixatives e.g. Methacarn, Bouins solution, ethanol and B5. |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
Alternative names
CD331, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, FGFR1, Fibroblast growth factor receptor 1, FGFR-1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, bFGF-R-1, FLT-2
Recommended products
Anti-FGFR1 antibody (ab10646) is a rabbit polyclonal antibody that is used to detect FGFR1 in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Over 60 publications
- Trusted since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FGFR1 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P11362
- Purification technique
- Affinity purification Immunogen
- Specificity
ab10646 does not react with human FGFR2 and FGFR3.
- Concentration
- Purification notes
The product is an affinity-purified antibody prepared from pooled sera.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-FGFR1 antibody (ab10646) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trusted by the scientific community
Anti-FGFR1 (ab10646) was first used in a scientific publication in 2004 and has been cited over 60 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGFR1 also known as fibroblast growth factor receptor 1 is a protein with a molecular weight of approximately 92 kDa. It is a receptor tyrosine kinase that binds to fibroblast growth factors (FGFs) triggering a cascade of downstream signaling pathways. FGFR1 is widely expressed in various tissues including the brain skeletal muscle and the cardiovascular system. The FGFR1 protein plays an essential role in cellular processes such as proliferation differentiation and survival.
- Biological function summary
FGFR1 is significant in embryonic development and tissue repair. It does not function alone; rather it forms a complex with fibroblast growth factors and heparan sulfate proteoglycans facilitating receptor dimerization and autophosphorylation. FGFR1 is involved in bone growth angiogenesis and wound healing processes. The interaction of FGFR1 with these complex factors ensures the precise regulation of these critical physiological events in the body.
- Pathways
FGFR1 participates actively in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are vital for transmitting signals from the cell surface to the DNA in the cell nucleus regulating gene expression and affecting cell cycle progression. FGFR1 works alongside similar proteins like FGFR2 influencing cell fate decisions. By modulating these pathways FGFR1 ensures a proper response to environmental signals which maintains homeostasis and adapts to physiological needs.
- Associated diseases and disorders
FGFR1 is implicated in conditions such as cancer and skeletal dysplasias. Abnormal FGFR1 signaling due to mutations or overexpression can lead to tumorigenesis contributing to the development of cancers like breast and lung cancer. The protein FGFR2 is often evaluated in parallel for similar oncogenic activities. Moreover FGFR1-related dysregulation in bone development can result in skeletal disorders highlighting its importance in both normal physiology and pathological conditions. Anti-FGFR therapies and FGFR1 ELISA tools are being explored to better understand and tackle these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody (ab10646)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human umbilical cord sections, labeling FGFR1 with ab10646 at a 5 μg/mL concentration. The secondary used was a biotin-anti-Rabbit IgG Peroxidase.
Image from Wang YN et al., J Biol Chem. 2012 May 11;287(20):16869-79. Epub 2012 Mar 28. Fig 1.; doi: 10.1074/jbc.M111.314799; May 11, 2012 The Journal of Biological Chemistry, 287, 16869-16879.Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody (ab10646)
Immunofluorescence analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells, staining FGFR1 using ab10646.
Top row: Cells were either untreated (left) or treated with FGF2 (50 ng/ml) (right) for 60 minutes.
Bottom row: Cells were permeabilized with digitonin, and either untreated (left) or treated with FGF2 (50 ng/ml) (right) for 60 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody (ab10646)
Immunocytochemical immunofluorescence analysis of methanol fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labelling FGFR1 with ab10646 at a 1/100 dilution. Cells were fixed, then permeabilized. The secondary antibody used was a Goat Anti-Rabbit IgG, Cy3™ conjugate (red). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody (ab10646)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human umbilical cord sections, labeling FGFR1 with ab10646 at a 1/200 dilution.
Biotinylated secondary followed by avidin-HRP and AEC substrate, hematoxylin counterstain.
Antigen retrieval: 0.1% trypsin for 15 minutes at 37°C.
Western blot - Anti-FGFR1 antibody (ab10646)
Lane 3: Negative control.Lane 1: Western blot - Anti-FGFR1 antibody (ab10646) at 1/400 dilution
Lane 2: Western blot - Anti-FGFR1 antibody (ab10646) at 1/800 dilution
All lanes: Whole extract of 293T cells transfected wild type FGFR1
SecondaryAll lanes: Goat-Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody at 1/5000 dilution
Predicted band size: 92 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com