Skip to main content

Anti-FGFR1 antibody [EPR806Y] (ab76464) is a rabbit monoclonal antibody detecting FGFR1 in Western Blot, IP, ICC/IF. Suitable for Human.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 30 publications

- Trusted since 2009


Images

Western blot - Anti-FGFR1 antibody [EPR806Y] (AB76464), expandable thumbnail
  • Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] (AB76464), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [EPR806Y] (AB76464), expandable thumbnail
  • Western blot - Anti-FGFR1 antibody [EPR806Y] (AB76464), expandable thumbnail
  • Western blot - Anti-FGFR1 antibody [EPR806Y] (AB76464), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/190
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

1 product for Alternative Version

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

Alternative names

CD331, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, FGFR1, Fibroblast growth factor receptor 1, FGFR-1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, bFGF-R-1, FLT-2

Recommended products

Anti-FGFR1 antibody [EPR806Y] (ab76464) is a rabbit monoclonal antibody detecting FGFR1 in Western Blot, IP, ICC/IF. Suitable for Human.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 30 publications

- Trusted since 2009

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR806Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-FGFR1 antibody [EPR806Y] (ab76464) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of FGFR1?


Anti-FGFR1 [EPR806Y] (ab76464) specifically detects a band for FGFR1 (UniProt: P11362) at a molecular weight of 92kDa.

Trusted by the scientific community


Anti-FGFR1 [EPR806Y] (ab76464) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-FGFR1 antibody [EPR806Y] (ab76464) has been confirmed by Western blot testing in FGFR1 Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EPR806Y] also available for your convenience:
ab76464, Carrier free - Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free ab206382, Carrier free - Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free ab271861, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-FGFR1 antibody [EPR806Y] ab320422, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-FGFR1 antibody [EPR806Y] ab320423, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-FGFR1 antibody [EPR806Y] ab320424, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-FGFR1 antibody [EPR806Y] ab320425, PE - PE Anti-FGFR1 antibody [EPR806Y] ab320426, APC - APC Anti-FGFR1 antibody [EPR806Y] ab320427



Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FGFR1 also known as fibroblast growth factor receptor 1 is a protein with a molecular weight of approximately 92 kDa. It is a receptor tyrosine kinase that binds to fibroblast growth factors (FGFs) triggering a cascade of downstream signaling pathways. FGFR1 is widely expressed in various tissues including the brain skeletal muscle and the cardiovascular system. The FGFR1 protein plays an essential role in cellular processes such as proliferation differentiation and survival.

Biological function summary

FGFR1 is significant in embryonic development and tissue repair. It does not function alone; rather it forms a complex with fibroblast growth factors and heparan sulfate proteoglycans facilitating receptor dimerization and autophosphorylation. FGFR1 is involved in bone growth angiogenesis and wound healing processes. The interaction of FGFR1 with these complex factors ensures the precise regulation of these critical physiological events in the body.

Pathways

FGFR1 participates actively in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are vital for transmitting signals from the cell surface to the DNA in the cell nucleus regulating gene expression and affecting cell cycle progression. FGFR1 works alongside similar proteins like FGFR2 influencing cell fate decisions. By modulating these pathways FGFR1 ensures a proper response to environmental signals which maintains homeostasis and adapts to physiological needs.

Associated diseases and disorders

FGFR1 is implicated in conditions such as cancer and skeletal dysplasias. Abnormal FGFR1 signaling due to mutations or overexpression can lead to tumorigenesis contributing to the development of cancers like breast and lung cancer. The protein FGFR2 is often evaluated in parallel for similar oncogenic activities. Moreover FGFR1-related dysregulation in bone development can result in skeletal disorders highlighting its importance in both normal physiology and pathological conditions. Anti-FGFR therapies and FGFR1 ELISA tools are being explored to better understand and tackle these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464), expandable thumbnail

    Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: FGFR1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: MCF7 whole cell lysate (20 μg)
    Lane 4: SH-SY5Y whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76464 observed at 140 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab76464 was shown to specifically react with FGFR1 when FGFR1 knockout samples were used. Wild-type and FGFR1 knockout samples were subjected to SDS-PAGE. ab76464 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/mL and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)

    Predicted band size: 92 kDa

  • Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] (ab76464), expandable thumbnail

    Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] (ab76464)

    ab76464 (purified) at 1/190 immunoprecipitating FGFR1 in 10 μg A-204 (Human muscle rhabdomyosarcoma)whole cell lysate (Lanes 1 and 2, observed at 145 kDa). Lane 3 - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab76464 in A-204 whole cell lysate. For western blotting, ab76464 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] (ab76464)

    Predicted band size: 92 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [EPR806Y] (ab76464), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [EPR806Y] (ab76464)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling FGFR1 with at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in SH-SY5Y cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464), expandable thumbnail

    Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution

    All lanes: SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 92 kDa

    Observed band size: 145 kDa

  • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464), expandable thumbnail

    Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)

    All lanes: Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution

    All lanes: SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 92 kDa

    Observed band size: 130 kDa

  • Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464), expandable thumbnail

    Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464)

    Western blot: Anti-FGFR1 antibody [EPR806Y] (ab76464) staining at 1/500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab76464 was shown to bind specifically to FGFR1. A band was observed at 92 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in FGFR1 knockout cell line. To generate this image, wild-type and FGFR1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution

    Lane 1: Wild-type U-87 MG cell lysate at 20 µg

    Lane 2: FGFR1 knockout U-87 MG cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 92 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com