AB206382

Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal FGFR1 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD331, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, FGFR1, Fibroblast growth factor receptor 1, FGFR-1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, bFGF-R-1, FLT-2

2 Images

Lab

Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (AB206382)

ab76464 (purified) at 1/500 immunoprecipitating FGFR1 in 10 μg A-204 (Human muscle rhabdomyosarcoma)whole cell lysate (Lanes 1 and 2, observed at 145 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab76464 in A-204 whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76464).

All lanes:

Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] (<a href='/en-us/products/primary-antibodies/fgfr1-antibody-epr806y-ab76464'>ab76464</a>)

Predicted band size: 92 kDa

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Lab

Western blot - Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (AB206382)

This WB data was generated using the same anti-FGFR1 antibody clone [EPR806Y] in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (cat# ab76464).

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : FGFR1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : MCF7 whole cell lysate (20 μg)
Lane 4 : SH-SY5Y whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab76464 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab76464 was shown to specifically react with FGFR1 when FGFR1 knockout samples were used. Wild-type and FGFR1 knockout samples were subjected to SDS-PAGE. ab76464 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/mL and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FGFR1 antibody [EPR806Y] (<a href='/en-us/products/primary-antibodies/fgfr1-antibody-epr806y-ab76464'>ab76464</a>)

Predicted band size: 92 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR806Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab206382 is the carrier-free version of ab76464.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFR1 also known as fibroblast growth factor receptor 1 is a protein with a molecular weight of approximately 92 kDa. It is a receptor tyrosine kinase that binds to fibroblast growth factors (FGFs) triggering a cascade of downstream signaling pathways. FGFR1 is widely expressed in various tissues including the brain skeletal muscle and the cardiovascular system. The FGFR1 protein plays an essential role in cellular processes such as proliferation differentiation and survival.

Biological function summary

FGFR1 is significant in embryonic development and tissue repair. It does not function alone; rather it forms a complex with fibroblast growth factors and heparan sulfate proteoglycans facilitating receptor dimerization and autophosphorylation. FGFR1 is involved in bone growth angiogenesis and wound healing processes. The interaction of FGFR1 with these complex factors ensures the precise regulation of these critical physiological events in the body.

Pathways

FGFR1 participates actively in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are vital for transmitting signals from the cell surface to the DNA in the cell nucleus regulating gene expression and affecting cell cycle progression. FGFR1 works alongside similar proteins like FGFR2 influencing cell fate decisions. By modulating these pathways FGFR1 ensures a proper response to environmental signals which maintains homeostasis and adapts to physiological needs.

FGFR1 is implicated in conditions such as cancer and skeletal dysplasias. Abnormal FGFR1 signaling due to mutations or overexpression can lead to tumorigenesis contributing to the development of cancers like breast and lung cancer. The protein FGFR2 is often evaluated in parallel for similar oncogenic activities. Moreover FGFR1-related dysregulation in bone development can result in skeletal disorders highlighting its importance in both normal physiology and pathological conditions. Anti-FGFR therapies and FGFR1 ELISA tools are being explored to better understand and tackle these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

See full target information FGFR1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Technology in cancer research & treatment 20:15330338211004935 PubMed33783288

2021

FGFR1 Is Associated With Tamoxifen Resistance and Poor Prognosis of ER-Positive Breast Cancers by Suppressing ER Protein Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Lv,Shiming Guan,Mingjie Zhu,Hu Huang,Junqiang Wu,Xiaofeng Dai

Nature genetics 45:602-12 PubMed23583981

2013

Whole-genome sequencing identifies genetic alterations in pediatric low-grade gliomas.

Applications

Species

Unspecified reactive species

Jinghui Zhang,Gang Wu,Claudia P Miller,Ruth G Tatevossian,James D Dalton,Bo Tang,Wilda Orisme,Chandanamali Punchihewa,Matthew Parker,Ibrahim Qaddoumi,Fredrick A Boop,Charles Lu,Cyriac Kandoth,Li Ding,Ryan Lee,Robert Huether,Xiang Chen,Erin Hedlund,Panduka Nagahawatte,Michael Rusch,Kristy Boggs,Jinjun Cheng,Jared Becksfort,Jing Ma,Guangchun Song,Yongjin Li,Lei Wei,Jianmin Wang,Sheila Shurtleff,John Easton,David Zhao,Robert S Fulton,Lucinda L Fulton,David J Dooling,Bhavin Vadodaria,Heather L Mulder,Chunlao Tang,Kerri Ochoa,Charles G Mullighan,Amar Gajjar,Richard Kriwacki,Denise Sheer,Richard J Gilbertson,Elaine R Mardis,Richard K Wilson,James R Downing,Suzanne J Baker,David W Ellison

Clinical cancer research : an official journal of 18:6658-67 PubMed23082000

2012

Translating the therapeutic potential of AZD4547 in FGFR1-amplified non-small cell lung cancer through the use of patient-derived tumor xenograft models.

Applications

Unspecified application

Species

Unspecified reactive species

Jingchuan Zhang,Lin Zhang,Xinying Su,Ming Li,Liang Xie,Florian Malchers,ShuQiong Fan,XiaoLu Yin,YanPing Xu,Kunji Liu,Zhengwei Dong,Guanshan Zhu,Ziliang Qian,Lili Tang,Jakob Schöttle,Ping Zhan,Qunsheng Ji,Elaine Kilgour,Paul D Smith,A Nigel Brooks,Roman K Thomas,Paul R Gavine

The American journal of pathology 177:2379-86 PubMed20889570

2010

Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Darren C Tomlinson,Margaret A Knowles

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free

Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (AB206382)

Western blot - Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (AB206382)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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