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Rabbit Recombinant Monoclonal FGFR2 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 3 publications.


Images

Immunoprecipitation - Anti-FGFR2 antibody [EPR24075-418] (AB289968), expandable thumbnail
  • Western blot - Anti-FGFR2 antibody [EPR24075-418] (AB289968), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] (AB289968), expandable thumbnail
  • Flow Cytometry - Anti-FGFR2 antibody [EPR24075-418] (AB289968), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] (AB289968), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.

Alternative names

CD332, BEK, KGFR, KSAM, FGFR2, Fibroblast growth factor receptor 2, FGFR-2, K-sam, Keratinocyte growth factor receptor

Recommended products

Rabbit Recombinant Monoclonal FGFR2 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24075-418
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The fibroblast growth factor receptor 2 (FGFR2) also known as CD332 is a protein that functions as a transmembrane receptor tyrosine kinase. FGFR2 is important in signal transduction where it binds to fibroblast growth factors triggering downstream intracellular pathways. FGFR2 has two main isoforms FGFR2b and FGFR2c created through alternative splicing. The molecular weight of FGFR2 varies by isoform typically around 100-110 kDa. It gets expressed in various tissues including epithelial and mesenchymal tissues.

Biological function summary

FGFR2 plays a role in diverse cellular processes like proliferation differentiation and migration. As part of a receptor-ligand complex FGFR2 partners with fibroblast growth factors to initiate signaling cascades. It contributes to embryonic development tissue repair and angiogenesis. FGFR2b expression predominantly appears in epithelial cells while FGFR2c is found in mesenchymal cells reflecting its distinct role in tissue-specific signaling.

Pathways

FGFR2 integrates into key signaling networks especially the MAPK and PI3K/AKT pathways. It interacts with proteins like FRS2 and GRB2 which facilitate the activation of downstream signals promoting cell survival and growth. FGFR2's engagement in these pathways is critical for maintaining normal cellular functions and contributes to its involvement in regulating complex cellular responses.

Associated diseases and disorders

FGFR2 mutations have connections with certain cancers and craniosynostosis syndromes. Alterations in FGFR2 signaling pathways can lead to aberrant cellular growth and have been found in various cancers including breast and gastric cancer. In craniosynostosis syndromes mutations in FGFR2 affect bone development linking it with disorders like Apert syndrome. These conditions demonstrate the pivotal role of FGFR2 in maintaining cellular homeostasis and the consequences of its dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-FGFR2 antibody [EPR24075-418] (ab289968), expandable thumbnail

    Immunoprecipitation - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    FGFR2 was immunoprecipitated from KATO III (human gastric carcinoma) whole cell lysate with ab289968 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289968 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: KATO III (human gastric carcinoma) whole cell lysate 10 μg

    Lane 2: ab289968 IP in KATO III whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab289968 in KATO III whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

    All lanes: Immunoprecipitation - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    Predicted band size: 92 kDa

    Observed band size: 145 kDa, 85 kDa

  • Western blot - Anti-FGFR2 antibody [EPR24075-418] (ab289968), expandable thumbnail

    Western blot - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The antibody recognizes endogenous levels of total FGFR2 protein and a C-terminal truncated form K-sam (PMID:17505008).

    Negative control: SW620 (PMID: 24968263)

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-FGFR2 antibody [EPR24075-418] (ab289968) at 1/1000 dilution

    Lane 1: KATO III (human gastric carcinoma) whole cell lysate at 20 µg

    Lane 2: SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa

    Observed band size: 145 kDa, 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] (ab289968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling FGFR2 with ab289968 at 1/100 dilution, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). The section was incubated with ab289968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry - Anti-FGFR2 antibody [EPR24075-418] (ab289968), expandable thumbnail

    Flow Cytometry - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    Flow cytometric analysis of SW620 (human colorectal adenocarcinoma epithelial cell, Left) / KATO III (human gastric carcinoma, Right) cells labelling FGFR2 with ab289968 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: SW620 (PMID: 24968263). Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] (ab289968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labelling FGFR2 with ab289968 at 1/100 dilution, followed LeicaDS9800 (Bond™, Polymer Refine Detection). Membraneous staining on human gastric adenocarcinoma (PMID: 24518603). The section was incubated with ab289968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] (ab289968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    Immunohistochemical analysis of paraffin-embedded (A) KATO III cells labeling FGFR2 with ab289968 at 1/100 dilution followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on (A) KATO III cells. Negative control: no staining on (B) SW620 cells (PMID: 24968263). The section was incubated with ab289968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-FGFR2 antibody [EPR24075-418] (ab289968), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KATO III cells labeling FGFR2 with ab289968 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membrane staining in the KATO III cell line, while no staining was observed in the SW620 cell line. Negative control: SW620 (PMID: 24968263). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Western blot - Anti-FGFR2 antibody [EPR24075-418] (ab289968), expandable thumbnail

    Western blot - Anti-FGFR2 antibody [EPR24075-418] (ab289968)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) at 1/1000000 dilution.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-FGFR2 antibody [EPR24075-418] (ab289968) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

    Lane 2: FGFR2 KO HAP1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 85 kDa, 145 kDa

    Exposure time: 80s

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Product protocols

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