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AB318275

Anti-FGFR2IIIb/FGFR2b antibody [EPR28355-27] - BSA and Azide free

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Rabbit Recombinant Monoclonal FGFR2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human, Human samples.

View Alternative Names

CD332, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, FGFR-2, K-sam, Keratinocyte growth factor receptor

2 Images
Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR28355-27] - BSA and Azide free (AB318275)
  • WB

Supplier Data

Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR28355-27] - BSA and Azide free (AB318275)

This data was developed using ab318274, the same antibody clone in a different buffer formulation.

Negative control : HepG2 (PMID : 20093481).

Low expression : LoVo (PMID : 22701813).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR28355-27] (<a href='/en-us/products/primary-antibodies/fgfr2iiib-fgfr2b-antibody-epr28355-27-ab318274'>ab318274</a>) at 1/1000 dilution

Lane 1:

KATO III (human stomach spherical) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 85-135 kDa,36 kDa

false

Exposure time: 48s

Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR28355-27] - BSA and Azide free (AB318275)
  • WB

Supplier Data

Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR28355-27] - BSA and Azide free (AB318275)

This data was developed using ab318274, the same antibody clone in a different buffer formulation.

This antibody does not cross-react with human FGFR2IIIc.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR28355-27] (<a href='/en-us/products/primary-antibodies/fgfr2iiib-fgfr2b-antibody-epr28355-27-ab318274'>ab318274</a>) at 1/1000 dilution

Lane 1:

His-tagged human FGFR2IIIb fragment at 10 ng

Lane 2:

His-tagged human FGFR2IIIc fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa

false

Exposure time: 92s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28355-27

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody (AB10646)

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab318275 is the carrier-free version of ab318274.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFR2IIIb also known as FGFR2b is a variant of the fibroblast growth factor receptor 2 (FGFR2) with a molecular weight of approximately 120 kDa. This receptor is a critical component of the FGFR family and is expressed predominantly in epithelial tissues. It is one of the isoforms generated through alternative splicing distinguished by the inclusion of exon IIIb. FGFR2b plays a fundamental role in ligand binding with specific fibroblast growth factors leading to receptor dimerization and activation of its intrinsic tyrosine kinase activity.
Biological function summary

FGFR2b is important in the regulation of epithelial cell proliferation and differentiation. It participates in forming a receptor-ligand complex upon binding with its ligands which include FGF7 and FGF10. This receptor is involved in embryonic development organogenesis and tissue repair processes. FGFR2b influences cellular functions by activating downstream signaling cascades that dictate cell fate decisions. Its activity must be tightly regulated to ensure proper developmental and tissue-specific outcomes.

Pathways

FGFR2b is involved in the MAPK/ERK and PI3K/AKT signaling pathways two critical pathways associated with cell proliferation survival and differentiation. These pathways are activated upon FGFR2b dimerization and trans-autophosphorylation which recruits adaptor proteins like GRB2 and SOS1. FGFR2b shares common mechanistic connections with other growth factor receptors such as EGFR that cooperate in controlling cellular outcomes through these pathways. The FGFR2b signaling intersects with these pathways to ensure comprehensive cellular responses to environmental signals.

Defects or dysregulation in FGFR2b function are linked to various types of cancer including breast cancer and lung cancer. Abnormal FGFR2b signaling can lead to unchecked cell proliferation and tumor development. Additionally FGFR2b mutations are implicated in craniosynostosis syndromes like Apert syndrome where premature fusion of cranial sutures occurs. In these conditions FGFR2b is often found in conjunction with abnormal regulation of FGF ligands and interactions with proteins like FGF2. Targeting FGFR2b for therapeutic intervention is an active area of research in managing these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
See full target information FGFR2IIIb/FGFR2b
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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